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Complex microbial inoculant for preventing and treating genital tract infection

A technology of reproductive tract infection and compound bacterial agent, which is applied in the field of compound bacterial agent for prevention and treatment of reproductive tract infection, can solve the problems of reducing the abundance of probiotics, increasing the risk of recurrence, unfavorable to the stability of reproductive tract flora, etc. Denaliella concentration and effect of vaginal inflammation, great application prospect, good adhesion ability

Active Publication Date: 2022-05-31
JIANGSU WECARE BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Antibiotics can also reduce the abundance of probiotics while fighting against pathogenic bacteria, which is not conducive to maintaining the homeostasis of reproductive tract flora, thus increasing the risk of recurrence

Method used

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  • Complex microbial inoculant for preventing and treating genital tract infection
  • Complex microbial inoculant for preventing and treating genital tract infection
  • Complex microbial inoculant for preventing and treating genital tract infection

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0047] The preparation method of the bacterial liquid involved below is as follows:

[0048] Lactobacillus crispatus LCr86 bacterial liquid: inoculate the Lactobacillus crispatus cells into the MRS liquid medium with an inoculum amount of 2% of the total volume of the MRS liquid medium, and cultivate at 37°C for 24 hours. Adjust the bacterial concentration to 1 with the MRS liquid medium ×10 8 CFU / mL to obtain Lactobacillus crispatus bacterial liquid.

[0049] Lactobacillus gasseri LG08 bacterial liquid: inoculate the Lactobacillus gasseri cells in the MRS liquid medium with an inoculum amount of 2% of the total volume of the MRS liquid medium, and adjust the bacterial concentration with the MRS liquid medium after culturing at 37 °C for 24 hours 1×10 8 CFU / mL to obtain Lactobacillus gasseri bacteria solution.

[0050] Escherichia coli bacteria liquid: Inoculate Escherichia coli in the BHI liquid medium with an inoculum amount of 1% of the total volume of the BHI liquid med...

Embodiment 1

[0055] The present embodiment screens the bacterial strain against pathogenic bacteria infection from cheese, and the steps are as follows:

[0056] The cheese samples were collected, diluted 10 times with normal saline with a mass concentration of 0.9%, diluted 3 times, spread on MRS solid medium, cultured at 37 °C for 48 h, and then picked out typical bacteria and placed in MRS solid medium The surface was streaked and purified, and a single colony was picked and expanded at 37°C in MRS liquid medium, and then preserved with 30% glycerol.

[0057] The isolated single colony is screened according to its ability to inhibit the growth of Candida albicans, and the steps are as follows:

[0058] (1) Inoculate 10 μL of different Lactobacillus crispatus strains and 10 μL Candida albicans strains into a test tube containing 1 mL of MRS liquid medium, and incubate the test tube in a 37°C incubator for 24 hours to obtain a culture solution.

[0059] (2) Bacteriostatic experiment: Dil...

Embodiment 2

[0061] The present embodiment carries out morphological identification and 16S rRNA molecular biological identification of the strain obtained by screening in Example 1, and the steps are as follows:

[0062] (1) Morphological identification:

[0063] The strains were inoculated in MRS solid medium, cultured at 37 °C for 48 h, and observed under a microscope. The observation showed that the colonies were small, white, round, plump in the middle and scattered around.

[0064] (2) 16S rRNA molecular biology identification:

[0065] The strains stored at -80°C were taken out, inoculated into MRS liquid medium, and cultured at 37°C for 18 h. Pipette 1 mL of bacterial solution into a centrifuge tube, centrifuge at 6000 rpm for 10 min, remove the supernatant, and collect the bacterial cells.

[0066] The genome of the strain was extracted, and general bacterial primers were added for PCR amplification, and the amplified product was sent to Shanghai Sangon Bioengineering Co., Ltd. f...

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Abstract

The invention relates to a complex microbial inoculant for preventing and treating reproductive tract infection. The complex microbial inoculant for preventing and treating reproductive tract infection comprises a Lactobacillus crispatus LCr86 strain and a Lactobacillus gasseri LG08 strain, wherein the Lactobacillus crispatus LCr86 strain and the Lactobacillus gasseri LG08 strain are used for preventing and treating reproductive tract infection. The complex microbial inoculant can prevent and / or treat genital tract infection related diseases, and can be applied to preparation of products for preventing and / or treating genital tract infection related diseases.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and relates to a compound bacterial agent for preventing and treating reproductive tract infections. Background technique [0002] The female reproductive tract environment is often affected by various factors, resulting in the disorder of the flora, leading to the invasion of pathogenic bacteria, which leads to bacterial, mold, virus and other pathogenic bacterial infections, such as Escherichia coli, Gardnerella, and Candida albicans. The clinical symptoms of reproductive tract infections are: vaginal itching and burning, abnormal secretions, frequent urination and painful urination, and severe cases can lead to premature birth and cervical cancer. [0003] The female vagina is a complex micro-ecosystem composed of squamous epithelium and vaginal flora. The vagina of healthy women of reproductive age is usually dominated by Lactobacillus. There are usually four dominant species in the ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/747A61P31/02A61P31/04A61P31/10A61P15/02C12R1/225
CPCC12N1/20A61K35/747A61P31/02A61P31/04A61P31/10A61P15/02A61K2300/00Y02A50/30
Inventor 方曙光盖忠辉董瑶朱建国吴银琴
Owner JIANGSU WECARE BIOTECHNOLOGY CO LTD
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