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Polyphenol chitosan oligosaccharide compound as well as preparation method and application thereof

A technology of chitosan oligosaccharides and complexes, which is applied in the field of polyphenol chitosan oligosaccharides complexes and its preparation, can solve the problems of application limitations, polyphenols unstable and easy to decompose, etc., and achieve the improvement of anti-oxidation and the dissolution of polyphenols rate and antioxidant activity of the

Pending Publication Date: 2022-05-24
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, due to the instability and easy decomposition of polyphenols, its application in the fields of functional food and pharmaceuticals is limited.

Method used

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  • Polyphenol chitosan oligosaccharide compound as well as preparation method and application thereof
  • Polyphenol chitosan oligosaccharide compound as well as preparation method and application thereof
  • Polyphenol chitosan oligosaccharide compound as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1: a kind of polyphenol-chitosan oligosaccharide complex, the preparation method is as follows:

[0032] (1) Using buckwheat bran polyphenols, chitosan oligosaccharides (molecular weight 980Da) and naked oat flour as raw materials; (2) Enzymatic hydrolysis of buckwheat with 0.5% (unspecified in this application, all refer to mass fraction) glucoamylase Bran, incubate at 57.5°C for 190 min and then boil to inactivate the enzymes; (3) Extract polyphenols from buckwheat bran with ethanol, mix with chitosan oligosaccharide solution in an equal volume at a mass ratio of 1:1, shake on a vortex shaker for 10 min, and the mixed solution After centrifugation at 1,000 × g for 10 min, the supernatant was taken for freeze-drying to obtain the polyphenol-chitosan-oligosaccharide complex;

Embodiment 2

[0033] Example 2: A polyphenol-chitooligosaccharide complex comprising polyphenol extraction and chitooligosaccharide. The preparation method is as follows:

[0034] (1) Using buckwheat bran polyphenols, chitosan oligosaccharides (molecular weight: 980 Da) and naked oat flour as raw materials; (2) Enzymatic hydrolysis of buckwheat bran with 1.5% protease, culturing at 57.5°C for 190 min, and boiling to kill the enzymes; (3) ) Extract buckwheat bran polyphenols with ethanol, mix with chitosan oligosaccharide in a mass ratio of 1:1, mix the two in equal volumes, shake on a vortex shaker for 10 min, and centrifuge the mixture at 1,000 × g for 10 min, and then take the supernatant for freezing. drying to obtain polyphenol chitosan oligosaccharide complex;

Embodiment 3

[0035] Example 3: A polyphenol-chitooligosaccharide complex comprising polyphenol extraction and chitooligosaccharide. The preparation method is as follows:

[0036] (1) Using buckwheat bran polyphenols, chitosan oligosaccharides (molecular weight 980Da) and naked oat flour as raw materials; (2) Enzymatic hydrolysis of buckwheat bran with 1.5% cellulase, culturing at 57.5°C for 190 min, and boiling to inactivate the enzymes; (3) Extract buckwheat bran polyphenols with ethanol, mix with chitosan oligosaccharide in a mass ratio of 1:1, mix the two in equal volumes, shake on a vortex shaker for 10 minutes, and centrifuge the mixture at 1,000 × g for 10 minutes, and then take the supernatant freeze-drying to obtain polyphenol chitosan oligosaccharide complex;

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Abstract

The invention belongs to the field of food, and particularly relates to a polyphenol chitosan oligosaccharide compound as well as a preparation method and application thereof, and the preparation method comprises the following steps: (1) performing enzymolysis or fermentation treatment on buckwheat bran; and (2) extracting the buckwheat bran polyphenol treated in the step (1) with ethanol, mixing the obtained polyphenol solution with a chitosan oligosaccharide solution in a mass ratio of 1: (1-5), oscillating and centrifuging on an oscillator, taking supernatant, and freeze-drying to obtain the polyphenol and chitosan oligosaccharide compound. According to the polyphenol chitosan oligosaccharide compound disclosed by the invention, the total phenol content and the antioxidant activity of the buckwheat bran before and after enzymolysis treatment are determined, and the polyphenol dissolution rate and the antioxidant activity are greatly improved through compound enzymolysis. Meanwhile, the oxidation resistance of the polyphenol, the chitosan oligosaccharide and the polyphenol and chitosan oligosaccharide compound is measured, so that the oxidation resistance of the compound is remarkably improved.

Description

technical field [0001] The invention belongs to the field of food, and in particular relates to a polyphenol chitosan oligosaccharide complex and a preparation method and application thereof. Background technique [0002] Buckwheat bran is rich in polyphenolic compounds, which have antioxidant, antibacterial, hypoglycemic, antiviral, anticancer, antiradiation, reducing vascular permeability and fragility effects. Polyphenols play a major role in food preservation, extension of product shelf life, protection and enhancement of food nutrients. The enzymatic hydrolysis method has the advantages of simple operation, mild conditions, energy saving, high reaction efficiency and environmental friendliness, which is beneficial to industrial production. Enzymatic hydrolysis can better maintain or even improve the nutritional value of raw materials, and enzymatic hydrolysis of bran can significantly improve the dissolution and extraction rate of polyphenols. [0003] However, due to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/105A23L33/125A23L7/109A23L3/3562A23L3/3544
CPCA23L33/105A23L33/125A23L7/109A23L3/3562A23L3/3544A23V2002/00A23V2200/02A23V2250/2132
Inventor 曹汝鸽刘昕茹封珂玮赵晓昱
Owner TIANJIN UNIV OF SCI & TECH
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