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Purifying breeding method for Guangxi gilt-edged leeches

A technology of Phnom Penh leeches and breeding methods, which is applied in fish farming, resistance to vector-borne diseases, animal husbandry, etc., can solve the problems of difficulty in popularizing the application of live leeches, hidden safety hazards, and inconsistent quality and safety of live leeches. Eliminate interference from exogenous pathogenic bacteria, easy collection and storage, and avoid cross-infection of pathogenic bacteria

Active Publication Date: 2022-05-20
广西蛭郎中生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The application of living Phnom Penh leeches in Guangxi has been used since ancient times, and it can be used for skin flaps, skin grafts or other tissues that suffer from impaired venous circulation. However, the existing leech breeding techniques are based on outdoor field cultivation or ordinary indoor water tanks. Breeding is the main method, and the live leeches obtained from farming will carry some pathogenic bacteria, so the obtained live leeches cannot be directly used in medical treatment
[0003] In order to use the live leeches obtained from farming for medical treatment, it is necessary to disinfect the live leeches according to the user's own knowledge, which leads to the use of live leeches for medical treatment The quality and safety of leeches are not uniform, and there are potential safety hazards, which makes it difficult to promote the medical application of live leeches

Method used

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  • Purifying breeding method for Guangxi gilt-edged leeches
  • Purifying breeding method for Guangxi gilt-edged leeches

Examples

Experimental program
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Effect test

Embodiment 1

[0038] A method for purifying and cultivating leeches in Phnom Penh, Guangxi, comprising the following steps:

[0039] Step 1. Obtain the Phnom Penh leech in Guangxi. After 30 days of temporary support, use ciprofloxacin mixed with water to prepare a potion with a concentration of 0.15g / L every 10 days during the temporary support period to perform an antibacterial bath on the Phnom Penh leech. The medicated bath lasted for 48 hours, and the parents of Phnom Penh leech were obtained.

[0040] Step 2: Feeding the Phnom Penh leech parent until the individual leech is greater than or equal to 8 grams per piece, to obtain the mature individual of the parent leech.

[0041] In step 3, the mature individuals of the parent leech are cultivated to produce cocoons at a density of 200 / m2 to obtain second-generation leech cocoons; the second-generation leech cocoons are incubated after sterilization to obtain second-generation leech seedlings.

[0042] Step 4: Feeding the second-generatio...

Embodiment 2

[0045] On the basis of Example 1, ciprofloxacin was replaced with enrofloxacin.

Embodiment 3

[0047] On the basis of Example 1, ciprofloxacin was replaced with norfloxacin.

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Abstract

The invention discloses a purification breeding method for Guangxi gilt-edged leeches. The purification breeding method comprises the steps that the Guangxi gilt-edged leeches are obtained; carrying out temporary rearing for at least 30 days; feeding each leech until the weight of each leech is greater than or equal to 8g; carrying out cocoon production incubation according to the density of 190-210 cocoons per square meter; feeding each leech until the weight of each leech is greater than or equal to 8g; finally, fasting cultivation is carried out for at least 6 months; the second generation of leeches are subjected to antibacterial medicated bath once every 10 days during the temporary rearing period and the last month of fasting rearing, and the purified Guangxi gold-edged leeches are obtained; wherein the whole breeding period is carried out in a GMP workshop with the cleanliness of D level. The purification breeding method for the Guangxi gold-edged leeches is carried out in a D-level GMP breeding workshop, the influence of production factors on the leech quality is reduced to the maximum extent, through multiple times of antibacterial medicated bath, the interference of common pathogenic bacteria in the breeding process can be reduced to the maximum extent while the biological characteristics of the leeches are followed, and the breeding quality of the leeches is improved. And breeding the medical Golden-edged Leech in Guangxi province.

Description

technical field [0001] The invention belongs to the technical field of leech breeding, in particular to a method for purifying and cultivating leeches in Phnom Penh, Guangxi. Background technique [0002] The Phnom Penh leech in Guangxi belongs to the blood-sucking leech with high hirudin content. It is the most common leech species used as a medical leech for treating diseases. The application of living Phnom Penh leech in Guangxi has been in the folk since ancient times. It can be used in the case of skin flaps, skin grafts or other tissues suffering from venous circulation damage. However, the existing leech breeding techniques are all cultivated in outdoor fields or ordinary indoor water tanks. Breeding is the main method, and the live leeches obtained from breeding will carry some pathogenic bacteria, so that the obtained live leeches cannot be used directly in medical treatment. [0003] In order to use the cultured live leeches for medical treatment, it is necessary ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/40A23K20/147A23K20/142A23K20/174A23K50/80
CPCA01K61/40A23K20/147A23K20/142A23K20/174A23K50/80Y02A50/30
Inventor 覃壬贺嘉允李洪
Owner 广西蛭郎中生物科技有限公司
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