Method for synthesizing muscone in vitro by gene editing yeast

Abstract

The invention discloses a method for synthesizing musk ketone in vitro by gene editing yeast, which comprises the following steps: malonyl-acyl carrier protein and 2-ketoisovalerate form 2-methallyl-ACP under the action of thiamine metabolic regulatory protein, and 14-methyl-pentadecanoyl-coenzyme A is synthesized by extension under the action of fatty acid synthase FAS1 / FAS2, under the action of TES1, 14-methyl-pentadecanoic acid is generated; the 14-methyl-pentadecanoic acid is subjected to omega-hydroxylation under the action of CYP54A1, CYP54A13 or CPR2, so that omega-hydroxyl-14-methyl-pentadecanoic acid is formed; and the musk ketone is formed from the omega-hydroxy-14-methyl-pentadecanoic acid under the catalysis of lactone lipase. According to the invention, a brand-new muscone biosynthesis way is constructed, the yeast obtains the muscone synthesis capability through a gene editing technology, the synthesis yield can reach 843mg / L, the cost is low, and mass production can be realized.

Description

technical field [0001] The invention belongs to the technical field of muskone synthesis, and in particular relates to a method for synthesizing muskone in vitro by gene editing yeast. Background technique [0002] Muscone is not only an important component of musk, a precious traditional Chinese medicine, but also an important spice. At present, there are two main production methods of muscone: [0003] The first is by raising musk musk deer ( Moschus berezovskii ), by extracting incense from living bodies, that is, extracting musk from the dry secretion in the sachet of mature males through distillation to obtain muscone. However, this method has the problem that due to the lack of resources, inbreeding leads to species degeneration, which leads to a decrease in musk production; secondly, the secretion of male musk deer has an annual cycle, and the fragrance is secreted once in May to July in a year, and the annual production of musk Only about 10 grams. At the same ti...

AI Technical Summary

Problems solved by technology

Although the substrate raw materials are cheap in this method, the requirements for the synthetic environment are high and the route is complicated

[0005] In summary, the current methods of producing muskone, whether by cultivating forest musk deer or through chemical synthesis in various ways, have obvious shortcomings.

Obtaining muskone through artificial breeding of forest musk deer has few products, long cycle and high cost; chemical synthesis has disadvantages such as low yield, complicated operation, complicated route, high synthetic environment requirements, and long cycle.

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