Application of Hn gene-based regulation and control of aggregation migration behavior of oedaleus decorus asiaticus and locust treatment method

A gene regulation and car locust technology, applied in other methods, applications, and genetic engineering of inserting foreign genetic materials, can solve problems such as enhancing insect drug resistance, unfavorable environmental sustainable development, and environmental pollution

Active Publication Date: 2022-05-13
GRASSLAND RES INST OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, the more traditional and efficient pest control methods for the Asian carcus are usually achieved by using chemical pesticides. The use of chemical pesticides will lead to environmental pollution, and a large amount of pesticide residues will endanger the health of humans and animals.
In addition, the long-term use of chemical pesticides to control pests will enhance the resistance of insects. On the one hand, it hinders the effective control of pests, and on the other hand, it is not conducive to the sustainable development of the environment. Therefore, green and sustainable pest control technologies are urgently needed to be developed.

Method used

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  • Application of Hn gene-based regulation and control of aggregation migration behavior of oedaleus decorus asiaticus and locust treatment method
  • Application of Hn gene-based regulation and control of aggregation migration behavior of oedaleus decorus asiaticus and locust treatment method
  • Application of Hn gene-based regulation and control of aggregation migration behavior of oedaleus decorus asiaticus and locust treatment method

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Embodiment Construction

[0034] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments. The following non-limiting examples can enable those skilled in the art to understand the present invention more fully, but are not intended to limit the scope of the present invention. The experimental methods that do not specify specific conditions in the examples are usually carried out according to conventional conditions, or according to the conditions suggested by the manufacturer.

[0035] The reagents used in the present invention are all commercially available reagents, the main reagents used in the present invention and their manufacturers: RNA extraction kit, reverse transcription kit (promega company, USA). 4. 2×Taq PCR Master Mix, DNA recovery reagent (Tiangen Company, China). pGEM-T Easy Vector (Takara, Japan). Plasmid DNA Extraction Kit (Tiangen Company, China). Escherichia coli DH5α competent cells (Tiangen Company, China). ...

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Abstract

The invention discloses an application of Hn gene-based regulation and control of aggregation and migration behaviors of oedaleus decorus asiaticus and a locust control method, and discloses a key gene Hn for regulating and controlling deformation of oedaleus decorus asiaticus, and the Hn gene is used for regulating and controlling phenotypic plasticity of oedaleus decorus asiaticus. Hn gene transcriptional regulation is used for inhibiting the phenotype of the oedaleus decorus asiaticus from being changed from a scattered type to a gregarious type; according to the invention, the Hn gene is utilized to design the double-stranded RNA to inject the oedaleus decorus asiaticus, so that the proportion of deformation of the oedaleus decorus asiaticus is obviously reduced, and the formation of oedaleus decorus asiaticus population individuals and outbreak of locust groups are effectively prevented. The Oedaleus decorus asiaticus phenylalanine hydroxylase gene Hn is regulated and controlled, and the phenotype of Oedaleus decorus asiaticus is changed from a green residence type body color to a brown migration type body color. According to the invention, the double-stranded RNA fragment is injected to inhibit the change of the resident type oedaleus asiaticus to the migratory flight type, so that the probability of outbreak and damage of the oedaleus asiaticus is reduced, and the purpose of effectively controlling the outbreak and damage of locusts is achieved.

Description

technical field [0001] The invention belongs to the technical field of control of grassland pests, and in particular relates to an application of Hn gene-based regulation of the gathering and migrating behavior of the Asian locust and a method for controlling the locusts. Background technique [0002] The Asiatic locust is an important grassland economic pest. The Asiatic locust is widely distributed in the northern grasslands. It mainly feeds on gramineous pastures and grass crops, causing serious harm to the northern grasslands and seriously threatening agricultural and animal husbandry production. Gramineae grasses such as Leymus chinensis, Stipa and Phyllostachys spp., as well as grass crops such as wheat, corn and sorghum, are damaged by the Asian locust. In the prior art, the more traditional and efficient pest control methods for the Asian carcus are usually achieved through the use of chemical pesticides. The use of chemical pesticides will lead to environmental poll...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/89C12N15/113C12N15/53A01K67/033
CPCC12N15/89C12N9/0071C12N15/1137C12Y114/16001A01K67/0339C12N2310/14A01K2227/706A01K2267/02
Inventor 高书晶韩海斌郭娜闫峰孙彦楠王玲玲张国英高海燕杨旭兵牛文远
Owner GRASSLAND RES INST OF CHINESE ACAD OF AGRI SCI
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