Application of Hn gene-based regulation and control of aggregation migration behavior of oedaleus decorus asiaticus and locust treatment method
A gene regulation and car locust technology, applied in other methods, applications, and genetic engineering of inserting foreign genetic materials, can solve problems such as enhancing insect drug resistance, unfavorable environmental sustainable development, and environmental pollution
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[0034] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments. The following non-limiting examples can enable those skilled in the art to understand the present invention more fully, but are not intended to limit the scope of the present invention. The experimental methods that do not specify specific conditions in the examples are usually carried out according to conventional conditions, or according to the conditions suggested by the manufacturer.
[0035] The reagents used in the present invention are all commercially available reagents, the main reagents used in the present invention and their manufacturers: RNA extraction kit, reverse transcription kit (promega company, USA). 4. 2×Taq PCR Master Mix, DNA recovery reagent (Tiangen Company, China). pGEM-T Easy Vector (Takara, Japan). Plasmid DNA Extraction Kit (Tiangen Company, China). Escherichia coli DH5α competent cells (Tiangen Company, China). ...
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