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Function and application of deubiquitinating enzyme ZRANB1 for regulating lipid metabolism

A functional and white fat technology, applied in the fields of application, metabolic diseases, medical preparations containing active ingredients, etc., can solve the problems of undisclosed ZRANB1 protein/gene promoting fat burning, obesity and related diseases, so as to avoid insufficient effectiveness, The effect of avoiding security

Active Publication Date: 2022-05-13
ACADEMY OF MILITARY MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Through searching in patent databases and journal literature databases, it can be seen that ZRANB1 protein / gene belongs to the prior art, but none of the prior art discloses the related uses of ZRANB1 protein / gene to promote fat burning, treat obesity and related diseases, etc.

Method used

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  • Function and application of deubiquitinating enzyme ZRANB1 for regulating lipid metabolism
  • Function and application of deubiquitinating enzyme ZRANB1 for regulating lipid metabolism
  • Function and application of deubiquitinating enzyme ZRANB1 for regulating lipid metabolism

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 Zranb1 Gene knockout mice have significantly reduced white adipose tissue content

[0045] Build with Loxp_Cre strategy Zranb1 Knockout mice, through PCR identification of mouse genotypes and Western blot detection of isolated mouse embryonic fibroblasts (MEFs), confirmed that ZRANB1 protein was completely eliminated in knockout mice. During the analysis of mouse phenotypes it was found that Zranb1 The body weight of knockout mice was significantly lighter than that of wild-type mice of the same age ( figure 1 ).

[0046] for corroboration Zranb1 Weight loss in knockout mice is mainly caused by loss of adipose tissue, choose normal feeding Zranb1 The wild and gene knockout male mice were dissected and analyzed, and the brain, heart, lung, liver, kidney, and other tissues and organs of the mice were taken out for weighing and comparative analysis. The results found that Zranb1 The weight of major tissues and organs including brain, heart, lung, kidney and ...

Embodiment 2

[0047] Example 2 Zranb1 Fat-burning gene expression is significantly elevated in white fat of knockout mice

[0048] to confirm Zranb1 The expression of fat-burning genes in white adipose tissue of knockout mice, extracted Zranb1 The white adipose tissue (scWAT and epWAT) RNA of wild-type and gene knockout mice was reverse-transcribed to obtain cDNA, and then the expression of fat burning (Thermogenic genes) and lipogenesis (Adipogenic genes) related genes were detected by qPCR. The results showed Zranb1 The fat-burning genes in the white adipose tissue of gene knockout mice were significantly overexpressed ( Figure 5 ),illustrate Zranb1 After the protein expression is reduced, it can promote the rapid burning of white fat in the body.

[0049] The specific implementation steps are:

[0050] 1.1 Adipose tissue RNA extraction

[0051] (1) Tissue homogenization: mouse adipose tissue contains a large number of lipid droplets, which cannot be lysed by a tissue shaker. It is...

Embodiment 3

[0069] Example 3 H&E staining shows Zranb1 Enhanced Beige Coloration of White Adipose Tissue in Gene Knockout Mice

[0070] Both beige adipocytes and white adipocytes are differentiated from Myf5-negative progenitor cells, but their morphology and function are similar to brown adipocytes. Different from the large and stable lipid droplets in white adipocytes, beige adipocytes contain a large number of small particles of oil droplets, the number of mitochondria in the cells is significantly higher than that of white adipocytes, and they have the ability to induce the expression of UCP-1 gene. Therefore, beige adipocytes are also called brown-like adipocytes. To corroborate Zranb1 Whether the beige coloration of adipocytes in white adipose tissue in gene knockout mice is enhanced, will Zranb1 The scWAT and epWAT tissues of wild-type and knockout mice were subjected to H&E, and the results showed that: compared with wild-type mice, Zranb1 The particle oil droplets in the adip...

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Abstract

The invention discloses a function of a deubiquitination enzyme ZRANB1 for regulating lipid metabolism and application of the deubiquitination enzyme ZRANB1. The invention further discloses application of inhibiting ZRANB1 gene expression in preparing a product with at least one function of treating and / or preventing obesity, promoting white fat cell combustion, promoting slimming and losing weight and inhibiting accumulation / accumulation of subcutaneous and / or intra-abdominal white fat. Experiments prove that ZRANB1, as a newly discovered important fat metabolism regulation molecule, can significantly reduce the content of white fat of an organism after the protein level is reduced. The discovery of the ZRANB1 function provides an important scientific basis and application value for prevention of obesity diseases and treatment of slimming.

Description

technical field [0001] The invention relates to the field of biological medical care, in particular to an application of inhibiting the expression of the ubiquitinase ZRANB1 gene in regulating lipid metabolism. Background technique [0002] Obesity is a chronic metabolic disease in which the human body's long-term energy intake exceeds consumption, leading to excessive accumulation and abnormal distribution of white fat in the body, and reaching the level of endangering health. Obesity has become an epidemic in modern industrial society. With the development of industrialization, although readily available high-fat food satisfies human's long-standing desire for sugar and fat, it has also become a burden. The three iterations of the industrial revolution have completely changed people's diet and lifestyle, leading to an increase in the incidence of global obesity year by year, which has become a worldwide health problem. Worldwide obesity has nearly tripled since 1975, acco...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/00A61P3/04C12N9/48C12N15/57
CPCA61K45/00A61P3/04C12N9/485C12Y304/19012
Inventor 张令强李洪昌李超男崔春萍翟文静
Owner ACADEMY OF MILITARY MEDICAL SCI
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