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Wood-rotting fungus liquid culture medium and preparation method thereof

A liquid culture medium and wood-decaying fungus technology, applied in the fields of botanical equipment and methods, cultivation, plant cultivation, etc., can solve the problems of affecting the production process, poor strain activity, slow recovery of mycelium, etc., to improve product quality, Fast recovery and strong mycelium

Pending Publication Date: 2022-05-10
LUDONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the process of liquid culture of bacteria, the formula of the medium is the key. If there is a problem with the liquid medium, it is easy to cause problems such as slow recovery of mycelia, easy pollution, and poor vitality of the bacteria, which will affect the entire production process and cause great harm to the enterprise. big loss

Method used

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  • Wood-rotting fungus liquid culture medium and preparation method thereof

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Embodiment 1

[0026] A liquid culture medium for wood rot fungus, comprising the following components in mass concentration (g / L): 200 potatoes, 10 glucose, 6 yeast extract powder, 0.3 potassium chloride, 0.1 magnesium sulfate heptahydrate, 0.1 potassium dihydrogen phosphate and Flammulina velutipes root powder 5, water 1000.

[0027] A method for preparing a wood rot fungus liquid culture medium, the method steps comprising: taking a certain amount of fresh Flammulina velutipes, cutting off the stalks and caps of the Flammulina velutipes, keeping the mushroom roots, weighing 5 mushroom roots of Flammulina velutipes, and treating them in an oven at 55°C 10h, take it out, grind and sieve, and prepare mushroom root powder; weigh 200% of potatoes, peel and cut into pieces, add 5 pieces of Flammulina velutipes root powder, boil for 15-20 minutes, cook until the potatoes are cooked but not rotten, use 4-6 filter with a layer of gauze, and take the filtrate; heat the filtrate to boiling, then add...

Embodiment 2

[0031] A liquid culture medium for wood-rot fungus, comprising the following components in mass concentration (g / L): 200 potatoes, 15 glucose, 6 yeast extract powder, 0.3 potassium chloride, 0.1 magnesium sulfate heptahydrate, 0.1 potassium dihydrogen phosphate and Flammulina velutipes root powder 10, water 1000.

[0032] A method for preparing a wood rot fungus liquid culture medium, the method steps comprising: taking a certain amount of fresh Flammulina velutipes, cutting off the stems and caps of the Flammulina velutipes, keeping the mushroom roots, weighing 8 mushroom roots of Flammulina velutipes, and treating them in an oven at 55°C 10h, take it out, grind and sieve to prepare mushroom root powder; weigh 200% of potatoes, peel and cut into pieces, add 8 pieces of Flammulina velutipes root powder, boil for 15-20 minutes, cook until the potatoes are cooked but not rotten, use 4-6 Filter with a layer of gauze, and take the filtrate; heat the filtrate to boiling, then add g...

Embodiment 3

[0036] A kind of liquid culture medium for wood rot fungus, comprising the components of following mass concentration (g / L): 200% of potato, 20% of glucose, 6% of yeast extract powder, 0.3% of potassium chloride, 0.1% of magnesium sulfate heptahydrate, 0.1% of potassium dihydrogen phosphate and Flammulina velutipes mushroom root powder 15, water 1000.

[0037] A method for preparing a wood rot fungus liquid culture medium, the method steps comprising: taking a certain amount of fresh Flammulina velutipes, cutting off the stems and caps of the Flammulina velutipes, keeping the mushroom roots, weighing 15% of Flammulina velutipes roots, and treating them in an oven at 55°C 10h, take it out, grind and sieve to prepare mushroom root powder; weigh 200% of potatoes, peel and cut into pieces, add 10% of the enoki mushroom root powder, boil for 15-20 minutes, cook until the potatoes are cooked but not rotten, use 4-6 Filter with a layer of gauze, and take the filtrate; heat the filtra...

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Abstract

The invention relates to a wood-rotting fungus liquid culture medium and a preparation method thereof. The wood-rotting fungus liquid culture medium is characterized by comprising the following components in mass concentration (g / L): 200 g of potatoes, 10-20 g of glucose, 6-7 g of yeast extract powder, 0.3-0.6 g of potassium chloride, 0.1-0.4 g of magnesium sulfate heptahydrate, 0.1-0.2 g of monopotassium phosphate, 5-15 g of flammulina velutipes root powder and 1000 g of water. According to the method, waste needle mushroom roots in the production process of a factory are treated and utilized to be prepared into the liquid culture medium; meanwhile, the strain cultured by the liquid culture medium has the characteristics of fast hypha recovery, compact fungus balls and vigorous vitality. After inoculation, the strain is higher in adaptability, low in pollution rate and quick in germination, so that the production efficiency of a factory is improved, the production cost is saved, and the income of an enterprise is increased.

Description

technical field [0001] The invention relates to the field of liquid culture medium for bacteria, in particular to a wood rot fungus liquid culture medium and a preparation method thereof. Background technique [0002] Flammulina velutipes is a common edible fungus, including the root, handle and cap of Flammulina velutipes. The cap is smooth and tender, the handle is crisp, nutritious and delicious, and is deeply loved by the public. In the process of industrial production of Flammulina velutipes, because the roots of Flammulina velutipes have a small amount of matrix, the taste is poor, and they are not suitable for eating. Usually, the roots of Flammulina velutipes are excised and sold, and a large amount of discarded Flammulina velutipes roots are produced in this process. The root length of Flammulina velutipes accounts for about 20% to 30% of the length of the whole Flammulina velutipes. These enoki mushroom roots are usually used as fertilizer, feed or directly discar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/20
CPCA01G18/20
Inventor 程显好王磊孙瑞祥杨树德李维焕盖宇鹏白杨薛力铭
Owner LUDONG UNIVERSITY
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