Test strip for detecting hepatitis B surface antigen and preparation method thereof

Abstract

The invention discloses a test strip for detecting hepatitis B surface antigen and a preparation method thereof, and the preparation method comprises the following steps: S1, preparing a sample pad treating fluid, and treating a glass fiber membrane through the sample pad treating fluid to prepare a sample pad; s2, preparing a gold-labeled pad treating fluid, and treating the glass fiber membrane through the gold-labeled pad treating fluid to obtain a gold-labeled pad; and S3, preparing monoclonal antibody immune gold for resisting the hepatitis B virus surface antigen, and spraying the prepared immune gold on the treated gold label pad according to 3 [mu] l / cm by using a gold spraying and membrane scratching all-in-one machine. Based on the immune lateral flow chromatography technology, instruments are not needed in the detection process, and whether the hepatitis B surface antigen exists in blood or not can be judged through naked eyes; the detection result is accurate, the detection time is short, and the body of a detected person cannot be injured; because the detection time is short, acute detection can be carried out on a patient before an operation, so that the protection of medical personnel is facilitated, and the transmission of infectious diseases is effectively blocked.

Description

technical field [0001] The invention relates to the technical field of hepatitis B test paper, in particular to a test paper for detecting hepatitis B surface antigen and a preparation method thereof. Background technique [0002] Hepatitis B virus (HBV), referred to as hepatitis B virus, is a DNA virus that easily causes humans and chimpanzees to be infected with hepatitis B virus. Hepatitis B virus is a chronic infectious disease and one of the most common infectious diseases in the world. One, while my country is a high-incidence area of ​​hepatitis B, there are many cumulative patients with hepatitis B, and the harm is serious. Hepatitis B virus is 100 times more contagious than the HIV virus that causes AIDS. The persistent infection of this virus may lead to diseases such as hepatitis B, liver cirrhosis and hepatocellular carcinoma. Because it is an infectious disease, hepatitis B virus carriers and patients People are vulnerable to different degrees of discrimination ...

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Problems solved by technology

[0004] For example, the application number CN202010598229.5 discloses a hepatitis B surface antigen detection kit, and the application number CN201911422123.3 discloses a hepatitis B surface antigen detection method, and a hepatitis B surface antigen detection kit. Specifically discloses a method using magnetic particle chemiluminescence, a method for detecting hepatitis B surface antigen It specifically discloses a method for detecting hepatitis B surface antigen by enzyme-linked immunosorbent technology, both methods have long waiting time and detection steps Many, need to rely on instruments, expensive testing costs and other disadvantages, making it difficult to popularize and use in primary hospitals

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