African swine fever virus p49 protein epitope and application thereof

Abstract

The invention relates to an antigenic epitope of an African swine fever virus p49 protein and application thereof, and belongs to the field of genetic engineering, the amino acid sequence of the capsid protein of the African swine fever virus p49 (pB438L) protein is used as a material, the antigenic epitope is predicted through an antigenic epitope database, and a corresponding short peptide is synthesized according to the amino acid sequence. African swine fever virus is utilized to infect swine positive serum to screen synthetic peptide capable of performing specific reaction with virus positive serum, antigen epitopes and GST are fused to express recombinant protein, and phages display multi-epitope antigens (virus-like particle display epitopes and VLP) to determine dominant antigen epitopes. Through prediction, immunological screening and identification, the fact that the African swine fever virus p49 protein has 13 epitopes is determined for the first time. The acquisition of the antigen epitopes lays a foundation for research and development of African swine fever detection reagents and kits thereof, preparation of monoclonal antibodies and safe and safe products for identifying and diagnosing strategic prevention and control such as epitope vaccines by taking the antigen epitopes as materials. Not only can huge economic benefits be generated, but also important social benefits are achieved.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to an antigenic epitope of African swine fever virus p49 protein and application thereof. Background technique [0002] African swine fever is an acute and severe infectious disease of pigs caused by African swine fever virus, with a mortality rate of up to 100%. The World Organization for Animal Health (OIE) lists it as an animal disease that must be notified, and my country lists it as a class of animal infectious diseases that must be prevented. Since it was introduced into my country in August 2018, the epidemic has spread throughout the country in less than a year, and the epidemic has not been eliminated so far, causing major economic losses to my country's pig industry. The epidemic has caused extremely serious pollution to the environment. As of the beginning of 2021, 22 strains have been isolated from the field, including attenuated strains with lar...

AI Technical Summary

Problems solved by technology

[0004] Based on this, for the African swine fever virus and its complexity, vaccine research is slow, there is a lack of safe, effective, and differentially diagnosable vaccines, and the country is in urgent need of prevention and control products.

It is necessary to adopt a new idea and find a new way to screen and identify the protective antigens of African swine fever virus, that is, to screen and identify protective antigen epitopes, so as to solve the plight of the large and complex African swine fever virus genome and unclear protective antigens, which lead to the lack of key materials for genetic engineering vaccine research

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