Mycobacterium tuberculosis inclusion body protein renaturation method and special renaturation buffer solution thereof

Abstract

The invention discloses a mycobacterium tuberculosis inclusion body protein renaturation method and a special renaturation buffer solution thereof. Experiments prove that the renaturation buffer solution provided by the invention can be used for renaturating mycobacterium tuberculosis protein W544, and the renaturated W544 protein can effectively stimulate splenic lymphocytes of a mouse infected by mycobacterium tuberculosis to generate immune response and does not stimulate splenic lymphocytes of a healthy mouse to generate immune response. Therefore, the renatured W544 protein has very good immunogenicity. The renaturation buffer solution and the mycobacterium tuberculosis inclusion body protein renaturation method established according to the renaturation buffer solution have important application value.

Description

technical field [0001] The invention belongs to the technical field of protein purification, and in particular relates to a method for renaturation of mycobacterium tuberculosis inclusion body protein and a special renaturation buffer thereof. Background technique [0002] Strengthening the research on the diagnosis, prevention and treatment of tuberculosis is of great significance to the control of the tuberculosis epidemic. Mycobacterium tuberculosis protein is widely used in the basic research and development of tuberculosis. Because the natural source of Mycobacterium tuberculosis protein is greatly limited, the use of genetic engineering technology has almost become the only effective means to obtain Mycobacterium tuberculosis protein. [0003] The expression of proteins in the E. coli system has the characteristics of easy operation, clear genetic background, and high expression levels. However, when E. coli is used to express Mycobacterium tuberculosis proteins, the e...

AI Technical Summary

Problems solved by technology

Inclusion body renaturation is a complex process, and its success largely depends on the various physical and chemical properties of the protein, especially the content of cysteine ​​in the protein sequence. The more cysteine ​​content, the greater the renaturation The more difficult it is, most cysteine-containing inclusion body proteins cannot be refolded by conventional methods such as dialysis and dilution, which will affect the progress of subsequent related research and product development

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