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Two triterpenoids in sabia parviflora and preparation method thereof

A technology for compounds and triterpenoids, which is applied to two triterpenoids in C. floridarum and the field of preparation thereof, can solve the problems of restricting the development and quality control of medicinal materials of C. floridarum and their preparations, and achieves less impurities and harm to human body. The effect of small sex and simple operation

Active Publication Date: 2022-04-22
CHONGQING MEDICAL & PHARMA COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, triterpenoids are an important class of compounds in nature, and have a wide range of physiological activities. However, there are few reports on the triterpenoids in Qingfengteng, which limits the development and quality control of the medicinal material of Qingfengteng and its preparations.

Method used

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  • Two triterpenoids in sabia parviflora and preparation method thereof
  • Two triterpenoids in sabia parviflora and preparation method thereof
  • Two triterpenoids in sabia parviflora and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1: the preparation of 2 kinds of triterpenoids in the little flower Qingfengteng

[0036] 1. Experimental method

[0037] 1) Extraction:

[0038] Take the dried stems and leaves of Teng japonica, crush them, add 5 times the weight of 95% ethanol, ultrasonically extract 3 times at a temperature of 60°C, each time for 4 hours, filter, combine the extracts, and concentrate the extracts to the total Extract one-tenth of the volume to obtain concentrate I;

[0039] Add an equal volume of purified water to the concentrated solution, and stir evenly to obtain a prepared solution, and place it overnight at room temperature, filter the prepared solution overnight with a Buchner funnel, collect the filtrate, and concentrate the filtrate to alcohol-free Taste, to obtain concentrated solution II;

[0040] 2) Extraction:

[0041] Dilute the concentrated solution II in step 1) by adding purified water twice its volume to obtain a diluted solution;

[0042] Extracting ...

Embodiment 2

[0051] Embodiment 2: screening experiment

[0052] 1. Experimental method

[0053] 1.1 Thin-layer chromatography was performed on S1 and S2 respectively, developed under the condition of dichloromethane: cyclohexane = 3:1, and the color was developed by spraying vanillin concentrated sulfuric acid.

[0054] 1.2 S2-1, S2-2, S2-3, and S2-4 were combined before thin-layer chromatography, and developed under the condition of cyclohexane: dichloromethane: methanol = 6:2:0.6, using vanillin Concentrated sulfuric acid spray color.

[0055] 2. Experimental results

[0056] 2.1 as figure 1 and figure 2 As shown, S1 has more mixed impurities and less purple-red spots, while S2 has purple-red spots in the range of Rf value 0.2-0.4, and this part of the sample has strong crystallinity, but the purity is low, and further silica gel column chromatography is required. Separation, so S2 was selected for the next step of purification.

[0057] 2.2 as image 3 As shown, a series of purp...

Embodiment 3

[0058] Example 3: Structural Identification

[0059] 1. Experimental method

[0060] The structures of compound 1 and compound 2 were identified by carbon NMR spectroscopy.

[0061] 2. Experimental results

[0062] like Figure 4 Shown, compound 1: white crystals (chloroform). 13 C-NMR (150MHz, CDCl 3 )δ: 38.03(C-1), 27.23(C-2), 79.06(C-3), 40.25(C-4), 54.24(C-5), 18.45(C-6), 32.48(C-7 ), 38.9(C-8), 54.88(C-9), 36.71(C-10), 125.29(C-11), 125.77(C-12), 138.1(C-13), 42.36(C-14) ,24.45(C-15),35.31(C-16),34.68(C-17),133.38(C-18),36.71(C-19),33.09(C-20),36.17(C-21), 38.03(C-22), 27.9(C-23), 15.09(C-24), 16.6(C-25), 17.96(C-26), 20.25(C-27), 25.42(C-28), 32.48 (C-29), 24.14 (C-30). The above data are consistent with the literature reports, so compound 1 was identified as 3β-hydroxyl-11,13(18)-oleanadiene;

[0063] like Figure 5 Shown, compound 2: white crystals (chloroform). 13 C NMR (150MHz, CDCl 3 )δ: 37.15(C-1), 28.27(C-2), 78.73(C-3), 38.94(C-4), 51.16(C-5), 18.37(C...

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Abstract

The invention discloses two triterpenoids in sabia parviflora and a preparation method thereof. The preparation method comprises the following steps: extracting chemical components in dry stems and leaves of sabia parviflora; sequentially extracting the triterpenoids with cyclohexane and ethyl acetate to obtain a crude total sample of the triterpenoids; according to the method, the total crude sample of the triterpenoids is separated and purified, the eluted S2-1 and S2-4 are respectively and repeatedly recrystallized to obtain the 3beta-hydroxyl-11, 13 (18)-oleanodiene and the 3beta-hydroxyl-9 (11), 12-oleanodiene which are natural products obtained from the plants of the genus for the first time, and the method is simple to operate and relatively high in yield.

Description

technical field [0001] The invention belongs to the technical field of medicines, and in particular relates to two kinds of triterpenoids in Teng xiaohuaqingfeng and a preparation method thereof. Background technique [0002] Xiaohua Qingfengteng, the name of traditional Chinese medicine. It is the stem and leaves of Sabia parviflora Wall. ex Roxb. It is mainly distributed in the southwest of Guizhou, the southeast to the southwest of Yunnan, the west and southwest of Guangxi; in the southwest of Guizhou, the Miao and Buyi people use the stems and vines of Xiaohua Qingfengteng to treat rheumatic joint pain, bruise pain, and hepatitis, etc. disease. Modern studies have shown that fenugreek contains flavonoids, alkaloids, triterpenoids, saponins and other components. Among them, triterpenoids are an important class of compounds in nature, and have a wide range of physiological activities. However, there are few reports on the triterpenoids in Qingfengteng, which limits the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00
CPCC07J63/008Y02A50/30
Inventor 朱仝飞闫志慧
Owner CHONGQING MEDICAL & PHARMA COLLEGE
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