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Method for creating mutation of rice seedlings through heavy ion beam radiation

A heavy ion beam and ion beam technology are applied in the field of mutation creation of rice seedlings irradiated by heavy ion beams, which can solve problems such as single sample state, and achieve the effect of increasing the number of plants.

Active Publication Date: 2022-04-22
INST OF MODERN PHYSICS CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The object of the present invention is to provide a kind of method that heavy ion beam irradiates rice seedlings to create mutations, and the present invention provides new ideas and approaches for rice breeding technology to overcome the Single flaw in sample status for radiation breeding

Method used

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  • Method for creating mutation of rice seedlings through heavy ion beam radiation
  • Method for creating mutation of rice seedlings through heavy ion beam radiation
  • Method for creating mutation of rice seedlings through heavy ion beam radiation

Examples

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Effect test

Embodiment 1

[0041] Embodiment 1, a method for creating mutations of rice seedlings irradiated by heavy ion beams, comprising the following steps:

[0042] (1) Seed disinfection: Select the same batch of rice variety Kitaake (Oryzasativa ssp japonica) without pests and diseases, with the same plumpness, and place its dry seeds in a hydrogen peroxide solution with a mass concentration of 3%, and disinfect them with Rinse with deionized water 4 times;

[0043] (2) Seedling preparation: place the sterilized rice seeds in a water-moistened petri dish and cultivate them for 5 days; germinate in a constant temperature incubator (32°C) without light from day 1 to day 4, and then move to Light culture room (temperature is 28-30°C, photoperiod is 14 hours light, 10 hours dark, light intensity is 250 μmol / m 2 Grow in s) for 1 day to obtain rice seedlings to be irradiated;

[0044] (3) radiation treatment: the rice seedling material obtained in the step (2) is placed in a water-moistened petri dish...

Embodiment 2

[0056] Embodiment 2, a method for creating mutations of rice seedlings irradiated by heavy ion beams, comprising the following steps:

[0057] (1) Seed disinfection: Select the same batch of rice variety Kitaake (Oryzasativa ssp japonica) without pests and diseases, with the same plumpness, and place its dry seeds in a hydrogen peroxide solution with a mass concentration of 3%, and disinfect them with Rinse with deionized water 5 times.

[0058] (2) Seedling preparation: place the sterilized rice seeds in a water-moistened petri dish and cultivate them for 5 days; germinate in a constant temperature incubator (32°C) without light from day 1 to day 4, and then move to Light culture room (temperature is 28-30°C, photoperiod is 14 hours light, 10 hours dark, light intensity is 250 μmol / m 2 s) for 1 day to obtain rice seedlings to be irradiated.

[0059] (3) Radiation treatment: the rice seedling material obtained in the step (2) is placed in a water-moistened petri dish, and th...

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Abstract

The invention discloses a mutation creation method for rice seedlings radiated by heavy ion beams. The method comprises the steps of seed disinfection, seedling preparation, radiation treatment, planting screening and mutation identification. According to the method, the rice seedlings germinating for several days are selected to be subjected to heavy ion beam mutagenesis treatment, the time of a heavy ion beam irradiation machine can be saved, meanwhile, rice mutation can be effectively induced, and the creation technology for inducing rice mutation through heavy ion beam radiation is enriched.

Description

technical field [0001] The invention belongs to the technical field of rice breeding, and in particular relates to a method for mutation creation of rice seedlings irradiated by heavy ion beams. Background technique [0002] Heavy ion beam radiation mutation breeding has played an important role and achieved fruitful results in the fields of gene resource expansion, germplasm innovation and crop breeding. Plants are irradiated with high-energy heavy ion beams, and the key target molecular DNA is damaged by single-strand, double-strand and DNA clusters. Misrepair causes genetic variation in individual genotypes of cells, and finally forms new plant varieties or excellent parent materials after screening. As an efficient physical mutagenesis source, heavy ion beam radiation has a mutagenic efficiency per unit dose that is 10 times higher than that of X-rays, γ-rays, electron beams and other low-energy-transfer line density (Linear Energy Transfer, LET) rays. Compared with the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/06A01G31/00
CPCA01H1/06A01G31/00Y02P60/14
Inventor 周利斌许超丽杜艳金文杰余丽霞曲颖
Owner INST OF MODERN PHYSICS CHINESE ACADEMY OF SCI
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