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Small and dense low-density lipoprotein cholesterol detection kit

A low-density lipoprotein and detection kit technology, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problem of low detection accuracy and precision, low resolution and specificity, and anti-interference. problems such as poor ability to achieve objective test results, strong anti-interference, and reduce interference

Active Publication Date: 2022-04-15
广西康柏莱科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional method has the disadvantages of cumbersome operation, low resolution and specificity, and long time. Among them, the homogeneous method is relatively simple and fast. Using the homogeneous method, the sdLDL-C in the sample to be detected is first protected, and the The lipoprotein cholesterol other than sdLDL-C in the sample to be tested is fully removed, and then the sdLDL-C in the sample is specifically detected. This method is easy to operate, so it has been well promoted
[0006] However, the existing small and dense LDL cholesterol detection kits have poor anti-interference ability, low detection accuracy and precision, and poor stability when opening the bottle And other issues

Method used

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  • Small and dense low-density lipoprotein cholesterol detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The small and dense low-density lipoprotein cholesterol detection kit provided in this embodiment includes reagent A and reagent B, and the composition of said reagent A and reagent B is as follows:

[0033] Reagent A:

[0034]

[0035] In the table: the mass ratio of lauryl glucoside, polyoxypropylene mannitol dioleate and macrogol glycerol laurate is 3:2:1.

[0036] Reagent B:

[0037]

[0038] Detection method: The detection method is two-point endpoint method, and the reaction direction is ascending reaction.

[0039] The detection conditions are: the detection main wavelength is 600nm, the secondary wavelength is 700nm, take 3μL of the sample to be tested, add 150uL of reagent A, mix well, keep the temperature at 37°C for 5min, read the absorbance value A1; then add 50μL of reagent B, mix well , after constant temperature at 37°C for 5 minutes, read the absorbance value A2, and the reaction absorbance OA=A2-A1.

[0040] sdLDL-C concentration (mmol / L) = sdL...

Embodiment 2

[0042] The small and dense low-density lipoprotein cholesterol detection kit provided in this embodiment includes reagent A and reagent B, and the composition of said reagent A and reagent B is as follows:

[0043] Reagent A:

[0044]

[0045]In the table: the mass ratio of lauryl glucoside, polyoxypropylene mannitol dioleate and macrogol glyceryl laurate is 1.5:4:1.

[0046] Reagent B:

[0047]

[0048] The detection method and detection conditions of this embodiment are the same as those in Embodiment 1.

Embodiment 3

[0050] The small and dense low-density lipoprotein cholesterol detection kit provided in this embodiment includes reagent A and reagent B, and the composition of said reagent A and reagent B is as follows:

[0051] Reagent A:

[0052]

[0053]

[0054] In the table: the mass ratio of lauryl glucoside, polyoxypropylene mannitol dioleate and lauric acid macrogol glyceride is 3:1:1.

[0055] Reagent B:

[0056]

[0057] The detection method and detection conditions of this embodiment are the same as those in Embodiment 1.

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Abstract

The invention discloses a small and dense low-density lipoprotein cholesterol detection kit, and belongs to the technical field of biological detection. The small and dense low-density lipoprotein cholesterol detection kit comprises a reagent A and a reagent B, wherein the reagent A comprises the following components: cholesterol esterase, cholesterol oxidase, peroxidase, a buffer solution, a stabilizer, a first surfactant and a preservative; and the reagent B comprises the following components: 4-aminoantipyrine, peroxidase, a buffer solution, a stabilizer, a second surfactant, a preservative and a reaction accelerator. The small and dense low-density lipoprotein cholesterol detection kit provided by the invention overcomes the defects of the existing small and dense low-density lipoprotein cholesterol detection kit, and has the advantages of strong anti-interference performance, high specificity, high accuracy, wide linear range, good bottle opening stability and the like; and a safe, rapid, accurate and pollution-free detection means is provided for detection of small and dense low-density lipoprotein cholesterol.

Description

technical field [0001] The invention belongs to the technical field of biological detection, in particular to a small and dense low-density lipoprotein cholesterol detection kit. Background technique [0002] Lipoproteins mainly play a transport role in the human body, delivering cholesterol to various tissues and organs of the body to perform their corresponding physiological functions. Chylomicrons (CM), very low-density lipoprotein (VLDL), low-density lipoprotein (LDL) and high-density lipoprotein (HDL) are four classes of lipoproteins in human plasma. [0003] Different types of lipoprotein cholesterol have different abilities to cause atherosclerosis. High-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) are mostly detected in clinical diagnosis. High-density lipoprotein cholesterol has a certain protective effect on arteries, and its high content lowers the risk of cardiovascular disease, while low-density lipoprotein cholestero...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/60C12Q1/44C12Q1/28C12Q1/26
Inventor 李名星张设熙莫智林韦佳志黄多全农雪静
Owner 广西康柏莱科技有限公司
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