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Application of platycodin D in treatment of acute lung injury caused by viral pneumonia

A technology for viral pneumonia and acute lung injury, applied in the field of biomedicine, can solve problems such as no reports on the application of Platycodon grandiflorin saponin D, and achieve the effect of improving symptoms and high safety

Pending Publication Date: 2022-04-15
SHANGHAI DERMATOLOGY HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the application of platycodon saponin D in the treatment of acute lung injury caused by viral pneumonia.

Method used

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  • Application of platycodin D in treatment of acute lung injury caused by viral pneumonia
  • Application of platycodin D in treatment of acute lung injury caused by viral pneumonia
  • Application of platycodin D in treatment of acute lung injury caused by viral pneumonia

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Experimental program
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Effect test

Embodiment 1

[0034] method:

[0035] To establish a severe infection model induced by influenza virus PR8 infection, 72 C57BL mice were adaptively fed for one week and divided into a normal group (12 mice) and a model group (60 mice) according to the random allocation method. Model preparation method: Mice were lightly anesthetized by inhalation of isoflurane, using a 100 μl pipette to draw 50 μl of diluted influenza virus solution, and inoculated through the nasal cavity to establish a model, normal group PBS, model group PR8, PR8+ positive drug group OSV (30mg / kg), PR8+ drug group PD (low dose: 1mg / kg, medium dose: 2mg / kg, high dose: 4mg / kg), administered two hours after modeling, the day of modeling is Day0, according to the mouse Body weight was given by intragastric administration, and after 7 days of continuous administration, the body weight of the mice was continued to be observed until the 14th day of continuous observation, and the daily body weight changes of the mice were recor...

Embodiment 2

[0038]Method: RAW264.7 cells were evenly seeded in 96-well plates, and cultured in a 37-degree cell incubator for 24h, 48h and 72h. Then add 10 μL of different concentrations of PD into the plate, use the same pipette to add 10 μL CCK8 solution to each well of the 96-well plate, incubate the 96-well plate in the incubator for 4 hours, use a microplate reader to measure 450nm Absorbance was used to detect cytotoxicity.

[0039] RESULTS AND CONCLUSION: Different incubation times found that PD below 10 μM did not show obvious cytotoxicity, and PD above 10 μM showed cytotoxicity ( image 3 ).

Embodiment 3

[0041] Methods: Influenza viruses are single-stranded RNA viruses. Imiquimod (Imiquimod, R837) is a synthetic single-stranded RNA that can mimic influenza virus nucleic acid, which is also a single-stranded RNA, in vitro. Therefore, we first used R837 to stimulate the mouse macrophage cell line Raw264.7 cells to construct a macrophage model of influenza virus infection.

[0042] RESULTS AND CONCLUSION: PD (1.25, 2.5, 5 or 10mM) significantly reduced the proinflammatory cytokines IL-6, TNF-α, human macrophage chemoattractant protein MCP in Raw264.7 cells induced by R837 (5mg / mL) -1, the secretion of type I interferon IFN-β, and at the same time, promote the secretion of anti-inflammatory cytokine IL-10 induced by R837 ( Figure 4 ).

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Abstract

The invention relates to application of platycodin D in treatment of acute lung injury caused by viral pneumonia. The invention establishes a severe infection mouse model induced by influenza virus infection, explores the application of platycodin D in treatment of acute lung injury caused by viral pneumonia, and finds that platycodin D can obviously improve the survival rate of the severe infection mouse model induced by PR8 infection and obviously relieve lung injury caused by infection. The invention also observes the influence of the platycodin D on the secretion of proinflammatory factors and anti-inflammatory factors of R837-induced Raw264.7 cells, mouse primary peritoneal macrophages and mouse macrophages directly infected by influenza virus PR8, wherein the R837-induced Raw264.7 cells, the mouse primary peritoneal macrophages and the mouse macrophages are directly infected by influenza virus PR8. The traditional Chinese medicine composition has the beneficial effect that an effective medicine is provided for treating acute lung injury caused by viral pneumonia, especially severe cases of viral pneumonia.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to the application of platycodon saponin D in the treatment of acute lung injury caused by viral pneumonia. Background technique [0002] Viral pneumonia refers to pulmonary inflammatory lesions caused by viral infection. Common pathogenic viruses include influenza virus, parainfluenza virus, adenovirus, coronavirus, rhinovirus, respiratory syncytial virus, and measles virus. Most outbreaks or sporadic epidemics occur in winter and spring. Patients and patients in the incubation period are the main source of infection, most of which are transmitted through air droplets, contact, etc. People with normal or abnormal immune functions are generally susceptible. In recent years, due to the continuous emergence of SARS coronavirus, avian influenza virus, and new coronaviruses, their outbreaks have resulted in a high mortality rate of viral pneumonia, making viral pneumonia an important disease...

Claims

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Application Information

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IPC IPC(8): A61K31/704A61P11/00A61P31/12A61P31/16A61P31/14C12N5/0786
Inventor 陈启龙李斌汤琛琛郑月娟宋建坤刘慧赵堃鹏张彩云卢曼晨胡友
Owner SHANGHAI DERMATOLOGY HOSPITAL
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