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Acoustic micro-fluidic system for cell fusion as well as preparation method and application of acoustic micro-fluidic system

A microfluidic system and cell fusion technology, applied in the field of acoustic microfluidic system for cell fusion and its preparation, can solve the problems of limiting wide application, increasing cell damage, low fusion efficiency, etc., achieving simple operation and high repeatability , the effect of low heat production

Active Publication Date: 2022-04-12
SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Due to the complex operation of this technology, the introduction of magnetic nanoparticles and nanowires increases the damage to cells, and the fusion efficiency is low, which greatly limits its wide application.

Method used

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  • Acoustic micro-fluidic system for cell fusion as well as preparation method and application of acoustic micro-fluidic system
  • Acoustic micro-fluidic system for cell fusion as well as preparation method and application of acoustic micro-fluidic system
  • Acoustic micro-fluidic system for cell fusion as well as preparation method and application of acoustic micro-fluidic system

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Experimental program
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Embodiment 1

[0048] 1. PDMS cavity preparation and bonding

[0049] figure 1 (a-e) shows the fabrication process of PDMS.

[0050] (1) Pretreatment: Remove residual impurities on the surface of the silicon substrate, such as dust and organic adsorbents, by pickling, alcohol washing, and water washing, and finally place the silicon wafer in a clean place to dry.

[0051] (2) Coating and pre-baking: use a coating machine to spin-coat SU-8(50) negative photoresist, 3000rpm, 30s, the thickness of SU-8(50) is about 50μm. After coating, the silicon wafer was placed horizontally on a heating plate at 90°C for 1 hour to allow the solvent in the photoresist to evaporate, so as to enhance the adhesion between the photoresist and the silicon wafer, and obtain figure 1 The graph in (a).

[0052] (3) Exposure and development: take the film that has already made graphics such as figure 1 (b) placed in figure 1 In (a), the photoresist is exposed through an exposure machine, and the exposure dose is ...

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Abstract

The invention discloses an acoustic micro-fluidic system for cell fusion as well as a preparation method and application thereof, and belongs to the technical field of cell fusion. The acoustic micro-fluidic system comprises a signal generator, a power amplifier, a PDMS cavity, a micro-injection pump, a pipeline, an EP tube, a cell recovery container and a bulk wave transducer / acoustic surface wave transducer. The side wall / bottom of the PDMS cavity channel is provided with equal and staggered microporous structures. The invention also provides a preparation method of the PDMS cavity in the acoustic micro-fluidic system and application of the acoustic micro-fluidic system in cell fusion, organoid fusion and 3D culture cell or body induction cell fusion. The system disclosed by the invention is extremely low in heat production, simple to operate, high in repeatability, strong in stability and applicable to fusion of homologous and non-homologous cells. The method is not only suitable for fusion of two cells, but also suitable for fusion of a plurality of cells, and can be widely applied to various types of cells.

Description

technical field [0001] The invention belongs to the technical field of cell fusion, and in particular relates to an acoustic microfluidic system for cell fusion and its preparation method and application. Background technique [0002] Cell fusion is one of the most fundamental processes in development, tissue repair and disease pathogenesis. Cell fusion begins with membrane fusion, in which two or more separate lipid membranes merge into a continuous bilayer through specific biological interactions, forming heterogeneous or homogeneous cells. Due to the hybrid vigor of fused cells, cell fusion technology has become a powerful tool in various biotechnologies, such as the production of monoclonal antibodies and improved breeding. Stem cell production, functional cell transplantation and cancer immunotherapy. However, the existing cell fusion technology has problems such as high cost, complicated operation, high cytotoxicity, low fusion efficiency and easy inactivation of bio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00C12M3/00C12M1/42
Inventor 孟龙刘秀芳郑海荣牛丽丽荣宁
Owner SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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