Preparation method of semi-solid armillaria mellea strain

Abstract

The invention provides a preparation method of a semi-solid armillaria mellea strain, which is characterized in that an armillaria mellea stock culture medium is prepared into a'jelly 'semi-solid state and contains solid and liquid, so that the germination speed, the growth speed and the purity (yield) of an armillaria mellea stock strain on the stock culture medium are remarkably improved; meanwhile, when the armillaria mellea cultivar is prepared, a sealing mulch culture medium is prepared, so that the germination speed and the growth speed of stock seeds on the cultivar culture medium are obviously increased, the stock seeds are quickly sealed, infectious microbe pollution is reduced, and the yield of the cultivar is increased by 5-8%; the hypha growth temperature is regulated and controlled in a segmented manner when the armillaria mellea cultivar is cultured, namely, the earlier-stage culture temperature is 15-18 DEG C, the middle-later-stage culture temperature is 22-24 DEG C, and the preservation temperature is controlled to be 4-8 DEG C. According to the process, scientization, uniformization and precision of armillaria mellea strain production are achieved, and a solid foundation is laid for large-scale and industrial strain production.

Description

technical field [0001] The invention belongs to the field of seed production of edible and medicinal fungus strains, in particular to a preparation method of semi-solid armillaria strains. Background technique [0002] Armillariella mellea (Armillariella mellea) is an edible and medicinal fungus belonging to the kingdom Fungi, Basidiomycota, Phytomycetes, Agaricales, Mildaceae, and Armillaria genus. Armillaria armillaria has a close relationship and co-evolution with Gastrodia elata, Polyporus and other organisms in nature. In the cultivation of Gastrodia elata and Polyporus umbilicalis, it is first necessary to produce high-quality or high-yield Armillaria strains, and to produce high-quality, high-yield Armillaria strains. On the one hand, it determines the economic benefits of strain manufacturers. On the other hand, it also directly affects or determines the yield and quality of Gastrodia elata and Polyporus chinensis cultivation, and is related to the economic benefits...

AI Technical Summary

Problems solved by technology

This formula and method are used for the production of the original species of Armillaria armillaria and the transfer of cultivated species. In addition to the long cultivation time, there are also two obvious disadvantages or deficiencies:

Therefore, if there is fungal infection in the commonly used solid culture medium, it is difficult to detect it from the outside.

[0005] Second, it is inconvenient to inoculate and susceptible to miscellaneous

After this commonly used solid substrate is covered with mycelium, when people transfer cultivars, that is, "cut into pieces", it is not easy to cut or separate, so that the inoculation speed is not fast, and more importantly, when "cutting or separating" the strains During the process, if you don’t pay attention to it, it will easily cause the inoculation feeling mixed, and it is very easy to cause the strain to become fine powder and affect the germination effect, which will affect the quality and efficiency of Armillaria strain production

Images