Novel goose astrovirus and duck tembusu virus multiplex fluorescent quantitative PCR detection primer probe set, kit and application

Abstract

The invention belongs to the technical field of virus detection, and particularly relates to a novel goose astrovirus (GoAstV) and duck Tembusu virus (DTMUV) multiplex fluorescent quantitative PCR detection primer probe group, a kit and application. According to the application, specific primers and probes are designed respectively aiming at an E gene of DTMUV and an ORF1b gene of GoAstV, and a fluorescent quantitative PCR method for simultaneously detecting DTMUV and GoAstV in the same system is established by optimizing reaction conditions. The method has the advantages of specificity, sensitivity and high efficiency.

Description

technical field [0001] The invention belongs to the technical field of virus detection, and in particular relates to a multiple fluorescent quantitative PCR detection primer probe set, kit and application of novel goose astrovirus and duck Tembusu virus. Background technique [0002] Since 2017, a number of goose farms in East China have had an outbreak of goose goose as the main clinical feature, mainly infecting goslings aged 4 to 16 days, resulting in a mortality rate of about 2% to 20%. Dead geese manifested as kidney disease. There is a large amount of urate deposition in the pleural peritoneum, the surface of the heart, and the ureter, which are whitish and swollen, causing great losses to the goose industry. After isolation and identification, it was found that the disease was caused by an infection of an astrovirus, which was quite different from the previously reported avian astrovirus. The homology of the amino acid sequence of the ORF2 gene of the capsid protein ...

AI Technical Summary

Problems solved by technology

[0002] Since 2017, a number of goose farms in East China have had an outbreak of goose goose as the main clinical feature, mainly infecting goslings aged 4 to 16 days, resulting in a mortality rate of about 2% to 20%. Dead geese manifested as kidney disease. White swelling, pleural peritoneum, heart surface, and ureter have a large amount of urate deposition, which has caused great losses to the goose industry.

[0004] At present, there have been researches on the establishment of a fluorescent quantitative PCR method for Tembusu virus and the establishment of a fluorescent quantitative PCR detection method for the new duck parvovirus SYBRGreenΙ, but the multiplex fluorescent quantitative PCR method for the new goose Astrovirus and duck Tembusu has not been reported.

Images