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ESR1 gene mutation multiple detection primer probe and kit thereof

A technology of multiple detection and primer probes, applied in the biological field, can solve problems such as inability to detect and achieve accurate results

Pending Publication Date: 2022-03-22
普瑞斯新(上海)生物医疗科技有限公司
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Problems solved by technology

The percentage of tumor-derived DNA fraction in total cfDNA is individually variable and often too low to detect

Method used

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  • ESR1 gene mutation multiple detection primer probe and kit thereof
  • ESR1 gene mutation multiple detection primer probe and kit thereof
  • ESR1 gene mutation multiple detection primer probe and kit thereof

Examples

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Embodiment

[0031] The ESR1 gene mutation multiple detection kit in this example includes upstream primer F-ESR1, downstream primer R-ESR1, wild-type probe WP-ESR1, mutant probe MP-Y537C for detecting the mutation of ESR1 gene exon 10 Y537C, Mutant probe MP-Y537N for detecting ESR1 gene exon 10 Y537N mutation, ESR1 gene exon 10 exon Y537S mutation detection probe MP-Y537S, ESR1 gene exon 10 D538G mutation detection probe MP-D538G and plasmids with Y537C, Y537N, Y537S and D538G mutations in exon 10 of the ESR1 gene.

[0032] The primers, probes and plasmids involved in this example were all designed by the laboratory technicians of Price New (Shanghai) Biomedical Technology Co., Ltd., and the synthesis unit was completed by Nanjing GenScript Biotechnology Co., Ltd. The primers and The nucleotide sequences of the probes are shown in Table 1.

[0033] Table 1 Primer probe characteristic table

[0034]

[0035] The primers are conventional primers, the probe is a modified MGB probe, the ...

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Abstract

The invention relates to a multiplex detection primer probe for ESR1 gene mutation and a kit thereof. The multiplex detection primer probe comprises an upstream primer, a downstream primer, a wild type probe, a mutant type probe MP-Y537C for detecting ESR1 gene No.10 exon Y537C mutation, a mutant type probe MP-Y537N for detecting ESR1 gene No.10 exon Y537N mutation, and a mutant type probe MP-Y537S for detecting ESR1 gene No.10 exon Y537S mutation. And the mutant probe MP-D538G is used for detecting the mutation of the No.10 exon D538G of the ESR1 gene. The ESR1 gene mutation multiple detection primer probe and the kit thereof are rapid and efficient, the preparation of a standard substance is simple, the specificity and sensitivity of a detection system are high, the utilization rate of free DNA is high, and the accuracy is high.

Description

technical field [0001] The invention relates to an ESR1 gene mutation detection product, which belongs to the field of biotechnology. Background technique [0002] ESR1 (Estrogen Receptor1) gene encodes the human estrogen receptor alpha protein (Estrogen receptor alpha, ESRα), which, after combining with estrogen, activates a series of cycle reactions in cells to promote cell growth and proliferation. Activating mutations of the ESR1 gene are closely related to the occurrence and development of breast cancer. Among them, the most common mutation types of ESR1 gene are Y537S, Y537C, Y537N and D538G. These mutations alter the conformation of ESRα, allowing it to exhibit a persistently active state in the absence of estrogen binding. [0003] Breast cancer is one of the most common gynecological tumors. More than 70% of primary breast cancers are estrogen receptor (ER) positive, and many breast cancers initially respond to endocrine therapy. Although the development of new ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/156C12Q2600/16C12Q2600/166
Inventor 洪专潘文健
Owner 普瑞斯新(上海)生物医疗科技有限公司
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