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Preparation method of earthworm bioactive fulvic acid

A biologically active and fulvic acid technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of low conversion rate, high cost, and large proportion of fulvic acid production

Active Publication Date: 2019-09-06
黑龙江奥创生物科技发展有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] The present invention aims to solve four problems in the process of using poultry manure and crop straw as raw materials to convert fulvic acid into fulvic acid by biochemical method and extracting it: 1. Treatment of poultry manure and crop straw in the existing biochemical method 2. The existing biochemical treatment of poultry manure and crop stalks produces many other substances, such as nucleic acids, sugars, amino acids, proteins and water-soluble carbohydrates, etc. , and the proportion is relatively large; 3. Since the physical and chemical properties of these substances are similar to those of fulvic acid, the cost of enriching and extracting fulvic acid separately after mixing is relatively high; 4. Existing biochemical treatment Under low temperature conditions, the problem of extracting biochemical fulvic acid through fermentation treatment under low temperature conditions cannot be realized

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  • Preparation method of earthworm bioactive fulvic acid
  • Preparation method of earthworm bioactive fulvic acid
  • Preparation method of earthworm bioactive fulvic acid

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specific Embodiment approach 1

[0034] Specific embodiment one: the preparation method of a kind of earthworm bioactive fulvic acid of the present embodiment, it is realized according to the following steps:

[0035] 1. The crop stalks are mixed with livestock and poultry manure, and fermented, and the fermented product is used as earthworm bait; wherein, in the fermented process, a compound microbial bacterial liquid mixed with 5 kinds of microbial strains is added at an inoculation amount of 0.1%; the microorganisms are Fusarium oxysporum, Neurospora sp., Phanerochaete chrysosporium, Bacillus subtilis and Bacillus acideler;

[0036] The initial conditions of fermentation treatment are carbon-nitrogen ratio of 25-30:1, humidity of 45-55%, pH value of 6.5-7.0, temperature of 8-20°C, and drum rotation every 24 hours during the fermentation process Turn over and ferment until the fineness of the product is ≦5mm;

[0037] 2. Evenly put the earthworm bait obtained in step 1 into the bioreactor with earthworms f...

specific Embodiment approach 2

[0042] Specific embodiment 2: This embodiment differs from specific embodiment 1 in that: the livestock and poultry manure is a mixture of one or more of pig manure, sheep manure, and cow manure; crop straw is corn, rice, One or more mixed straws of soybeans and wheat. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0043] Specific embodiment three: the difference between this embodiment and specific embodiment one is that the mass ratio of pig manure to corn straw is 1:4, the mass ratio of pig manure to rice straw is 1:3.5, and the mass ratio of pig manure to soybean straw The ratio is 1:5, and the mass ratio of pig manure to wheat straw is 1:6. Others are the same as in the first embodiment.

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Abstract

The invention discloses a preparation method of earthworm bioactive fulvic acid, and relates to a preparation method of fulvic acid. The invention aims to solve the problems in the process of converting livestock manure and crop straws into fulvic acid through a biochemical method and extracting the fulvic acid. The existing biochemical treatment needs to meet the temperature conditions, and the obtained fulvic acid has low purity, poor stability and short shelf life. According to the method, a mixture of livestock manure and straw is processed by 5 earthworm species in cooperation with microbial biological fermentation to produce an earthworm bait, and the earthworm bait is fed with high-efficiency earthworm varieties bred through independent hybridization to obtain earthworm manure withspecific components, and then the earthworm manure is extracted to finally obtain the earthworm bioactive fulvic acid. The method is applied to the field of preparation of fulvic acid.

Description

technical field [0001] The invention relates to a preparation method of fulvic acid. Background technique [0002] Fulvic acid is mineral-derived fulvic acid: it is extracted from low-grade coal (peat, lignite and weathered coal), and its composition structure is relatively simple, mainly aromatic hydroxycarboxylic acids, so it has strong activity and stability , but the purification process is complicated, the cost is high, and a large amount of pollutants are produced during the process; [0003] Biochemical fulvic acid is extracted from the decomposed organic fertilizer obtained after the plant waste is processed by microbial fermentation. There are 4 problems in the production and purification process: [0004] (1) Under the existing biochemical treatment of poultry manure and crop straw, the conversion rate of fulvic acid is low; [0005] (2) There are many other substances produced by the existing biochemical treatment of poultry manure and crop stalks, such as nucle...

Claims

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Application Information

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IPC IPC(8): C12P39/00C12P1/02C12P1/04C08H99/00C07G99/00C12R1/77C12R1/645C12R1/125C12R1/07
CPCC12P39/00C12P1/02C12P1/04C08H99/00C12R2001/125C12N1/205C12R2001/07C12R2001/77C12N1/145C12R2001/645C07G99/00
Inventor 王维晨钱华丁志洋郭金铸张爽
Owner 黑龙江奥创生物科技发展有限公司
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