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Medicine for preventing and treating nocardia disease and application thereof

A nocardiosis and drug technology, which is applied in the field of prevention and treatment of nocardiosis by extracts of Chinese herbal medicines and their active ingredients, which can solve problems such as drug resistance, environmental and food safety issues, and long treatment courses, and achieve low The effect of drug resistance and broad application prospects

Active Publication Date: 2022-03-22
西北农林科技大学深圳研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main treatment method is antibiotics, but due to the long course of treatment, it is easy to lead to the emergence of drug resistance, and antibiotic residues will also cause environmental and food safety problems, so there is an urgent need for safe, effective and environmentally friendly control methods

Method used

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  • Medicine for preventing and treating nocardia disease and application thereof
  • Medicine for preventing and treating nocardia disease and application thereof
  • Medicine for preventing and treating nocardia disease and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] This example describes the determination of the minimum inhibitory concentration (MIC) of Magnolia officinalis extract and its active ingredients magnolol and honokiol on Nocardia.

[0024] (1) Experimental strains

[0025] A strain of Nocardia was isolated and purified from the viscera of largemouth bass infected with Nocardia, which was identified as N. Aquatic Science Laboratory.

[0026] (2) Bacteria culture

[0027] Prepare Soybean Casein Broth (TSB): Weigh 3.0g of tryptone, 1.0g of soytone, and 1.0g of sodium chloride, add 200mL of distilled water, heat to dissolve, cool to room temperature, and adjust the pH to about 7.3 with 5mol / L hydrochloric acid , autoclaved at 121°C for 15 minutes, and cooled to room temperature for later use.

[0028] The inoculation was carried out near the flame of the alcohol lamp on the ultra-clean table, and placed in a constant temperature incubator for 5 days at 28°C. Take 5mL of the cultured bacterial solution and place it in a...

Embodiment 2

[0040] This example illustrates the effect of Magnolia Bark Extract, Magnolia and Honokiol as feed additives on feeding largemouth bass to prevent Nocardia infection.

[0041] (1) Feed preparation

[0042] Take a certain amount of Magnolia officinalis extract, after 50% ethanol dissolves, evenly spray to basal feed (purchased from Guangdong Yuehai Feed Co., Ltd. California perch special feed, particle diameter 2.5mm, does not contain Magnolia officinalis extract and its active ingredient magnolol and honokiol), so that its content is respectively 250, 500, 1000mg / kg (Magnolol extract / feed).

[0043] Take a certain amount of magnolol, honokiol, enrofloxacin, and make the medicated feed of high, middle and low doses in the same way, and the medicament content is respectively 400, 200, 100mg / kg (Magnolia bark extract / feed). Store at 4°C after ethanol volatilizes naturally.

[0044] (2) Experimental method

[0045] Select healthy largemouth bass with an average body weight of...

Embodiment 3

[0050] In this example, magnolol extract and its active ingredients magnolol and honokiol were made into injections, and the effect of preventing Nocardia infection was determined.

[0051] (1) Drug solution configuration

[0052] Magnolia officinalis extract, magnolol, honokiol, and enrofloxacin were respectively prepared into injections with a concentration of 5 mg / mL in DMSO solution.

[0053] (2) Experimental method

[0054] Select healthy snakeheads with an average weight of 30±3.4g, and randomly divide them into control group, negative control group and drug treatment group (enrofloxacin, magnolol extract, magnolol, honokiol). Three treatment groups with low, medium and high concentrations were set up in the drug treatment groups, and each group had three parallels, and each parallel had 20 snakeheads. The negative control group was injected with 50 μL of sterile PBS solution, and each fish in the control group and drug treatment group was intraperitoneally injected wi...

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Abstract

The invention provides a nocardia antibacterial composition. The effective components of the composition for inhibiting nocardia comprise a mangnolia officinalis extract, or a combination of the mangnolia officinalis extract and magnolol, or a combination of the mangnolia officinalis extract and honokiol, or a combination of the mangnolia officinalis extract, the magnolol and the honokiol. The other nocardia antibacterial composition comprises effective components of magnolol, or honokiol, or a combination of magnolol and honokiol. The invention discloses the inhibition activity of the mangnolia officinalis extract and the active component magnolol and honokiol thereof on nocardia for the first time, has the advantages of high efficiency, safety, no residue, easiness in degradation, low drug resistance and the like, and provides a new treatment method and way for nocardia infection of fishes (aquatic animals).

Description

technical field [0001] The invention belongs to the technical field of aquaculture and relates to the prevention and treatment of nocardiosis, in particular to the prevention and treatment of nocardiosis by extracts of Chinese herbal medicines and active components thereof. Background technique [0002] Nocardiosis is a zoonotic chronic infectious disease caused by Nocardia, which poses a great threat to aquaculture and aquatic product safety. Nocardia is a Gram-positive bacterium widely distributed in soil and water environments. The Nocardia that attack fish mainly include Nocardia seriolae, Nocardia asteroides ( N. asteroides) and Nocardia salmonicida (N. salmonicida). Among them, Nocardia spp. is the most common fish pathogenic bacteria, which can cause more than 100000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000 into largemouth bass, snakehead, tilapia and maricultured yellowtail, pomfret, sea bream, large yellow croaker an...

Claims

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Application Information

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IPC IPC(8): A61K36/575A61K31/05A61P31/04A23K10/30A23K20/111A23K50/80
CPCA61K36/575A61K31/05A61P31/04A23K10/30A23K20/111A23K50/80A61K2300/00Y02A40/81
Inventor 王高学凌飞姜海峰仁宗易张念锟
Owner 西北农林科技大学深圳研究院
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