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Plant growth regulator, treatment method for improving cold resistance of banana fruits and verification method

A technology for plant growth regulators and treatment methods, applied in the direction of plant growth regulators, plant growth regulators, botanical equipment and methods, etc., can solve problems such as retention and unstable treatment effects, achieve low cost, and delay the occurrence of chilling damage , the effect of suppressing the relative conductivity

Pending Publication Date: 2022-03-22
ZHEJIANG WANLI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been reported that the application of a variety of exogenous substances can enhance the cold tolerance of banana fruits, such as jasmonic acid (JA), abscisic acid (ABA), epibrassinosteroids (EBR), and nitric oxide (NO). and practicability, most of the exogenous substances are only in the experimental stage, and the treatment effect is not stable

Method used

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  • Plant growth regulator, treatment method for improving cold resistance of banana fruits and verification method
  • Plant growth regulator, treatment method for improving cold resistance of banana fruits and verification method
  • Plant growth regulator, treatment method for improving cold resistance of banana fruits and verification method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Banana fruit processing:

[0044] Banana fruits with uniform maturity, no damage, and no pests and diseases were selected and randomly divided into three groups, 100 in each group.

[0045] The first group of banana fruits was soaked in 500mg / L ethephon water solution for 1min; took out and dried naturally at 25°C and relative air humidity of 85%; sealed at 22°C for 18h; unpacked and stored at 7°C In the incubator, as the ethylene treatment group.

[0046] The second group of banana fruits were soaked in distilled water for 1 min; taken out and dried naturally, the drying temperature was 25°C, and the relative humidity of the air was 85%; sealed at 22°C for 18h; unpacked and stored in a 7°C incubator, as low temperature treatment group.

Embodiment 3

[0048] Lipid extraction and determination of banana fruit:

[0049] The banana fruit samples in this example all use the samples in Example 1.

[0050] 1. Selection of test samples: Take untreated banana peels, and banana peels that have been stored for the 5th and 7th days of low temperature treatment and ethylene treatment as test samples, and the peels of each test sample have the same size.

[0051] 2. Lipid extraction: The lipid extraction process is carried out in a dark environment. Quickly freeze each test sample in liquid nitrogen; place it in 4 mL of isopropanol containing 0.01% butylated hydroxytoluene, and incubate at 75°C for 15 minutes; after cooling, soak the test sample in the mixture of isopropanol and place at -80 Freeze at ℃ overnight; after thawing, add 12mL chloroform:methanol:300mM ammonium acetate (30:41.5:3.5), shake and mix at room temperature for 24h; after standing for 20min, transfer the lipid extract to a 10mL glass tube , removed the aqueous pha...

Embodiment 4

[0061] Comparing the test results of Example 2 and Example 3, PA content can be used as a biomarker for banana fruit to respond to low temperature stress, and the determination of PA content can directly reflect the degree of chilling damage of banana fruit.

[0062] According to the above conclusions, the present embodiment provides a banana fruit cold tolerance verification method, comprising the following steps:

[0063] (1) Select the banana fruit with uniform maturity, no damage, no damage by disease and pest; soak the banana fruit in the ethephon aqueous solution of 200-1000mg / L for 0.5-5min; take it out and dry it naturally, the drying temperature is 22-28°C, The relative humidity of the air is 80%-90%; sealed and stored at 18-25°C for 16-24h; stored in a thermostat at 4-10°C;

[0064] (2) Get the pericarp of untreated banana fruit as test sample one, get the pericarp of banana fruit placed in an incubator for 5 days after processing according to step (1) as test sample...

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Abstract

The invention provides a plant growth regulator, a treatment method for improving the cold resistance of banana fruits and a verification method. The plant growth regulator ethephon is used for treating the banana fruits, ethylene gas released by the ethephon can delay cold injury of the banana fruits, meanwhile, the increase of relative conductivity and malondialdehyde content induced by low temperature is remarkably inhibited, and the reduction of activity of catalase, superoxide dismutase and peroxidase is inhibited; the method is of great significance to cold-chain logistics transportation of the banana fruits. Based on the theoretical research of membrane lipid metabolism, the invention further designs a verification method of the cold resistance of the banana fruits, the content of phosphatidic acid (PA) is used as a biomarker of the banana fruits responding to low-temperature stress, and the cold damage degree of the banana fruits can be directly reflected by measuring the content of PA.

Description

technical field [0001] The invention relates to the technical field of fruit storage and preservation, in particular to a plant growth regulator, a treatment method and a verification method for improving the cold resistance of banana fruits. Background technique [0002] As a typical tropical and subtropical fruit, banana is loved by people because of its high yield, sweet flesh and rich nutrition. However, banana fruit is a cold-sensitive fruit, and chilling damage is prone to occur when stored at a temperature below 13°C. Shelf life, and limit the storage and transportation of banana fruit. A better understanding of the physiological response to low-temperature storage of postharvest banana fruit is an urgent need for banana storage and transportation, and it also provides a scientific theoretical basis for improving postharvest banana fruit fresh-keeping technology. It has been reported that the application of a variety of exogenous substances can enhance the cold tole...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N57/20A01P21/00A23B7/154
CPCA01N57/20A23B7/154
Inventor 宋春波杨震峰周颖余维维张懿
Owner ZHEJIANG WANLI UNIV
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