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Method for identifying variety and authenticity of radix bupleuri based on terahertz spectrum technology

A spectral technology and terahertz technology, which is applied in the identification of Bupleurum species and authenticity based on terahertz spectroscopic technology, can solve the problems of long identification time, inaccurate identification of Bupleurum, and achieve rapid detection and identification, absorption coefficient spectrum and Data reduction and optimization, intuitive effect of curve characteristics

Pending Publication Date: 2022-03-15
太赫兹科技应用(广东)有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The present invention aims to overcome at least one defect (deficiency) of the above-mentioned prior art, and provides a method for identifying Bupleurum species and its authenticity based on terahertz spectroscopy, which is used to solve the inaccurate identification of Bupleurum in the prior art and the identification too long problem

Method used

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  • Method for identifying variety and authenticity of radix bupleuri based on terahertz spectrum technology
  • Method for identifying variety and authenticity of radix bupleuri based on terahertz spectrum technology
  • Method for identifying variety and authenticity of radix bupleuri based on terahertz spectrum technology

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Embodiment 1

[0068] Such as figure 1 As shown, this embodiment provides a method for identifying Bupleurum species based on terahertz spectroscopy, to identify different species of Bupleurum.

[0069] Specifically include the following steps:

[0070] S1. Prepare Bupleurum bupleurum samples of several known varieties;

[0071] In this embodiment, the specific operations of step S1 include: preparing Bupleurum samples of four varieties of Bupleurum chinensis, North Bupleurum, South Bupleurum and Tibetan Bupleurum. Shake and pulverize for 3 minutes, pass through a 200-mesh sieve, dry the powder at 60°C for 2 hours, let it cool, accurately weigh 0.2g of the powder, place it on a tableting mold, press with 24MPa for 1min, and press it into a circular sheet with a diameter of 13mm , record the thickness of the sample sheet after tableting, and press 10 tablets of each variety of Bupleurum.

[0072] S2. Scanning and measuring the prepared Bupleurum samples with a terahertz time-domain spectro...

Embodiment 2

[0085] Such as Figure 8 As shown, this embodiment provides a method for identifying Bupleurum species based on terahertz spectroscopy technology to identify different species of Bupleurum. The steps of the method are roughly the same as those in Example 1. The difference is that step S3 also includes:

[0086] S31. Perform spectral quantization processing on the acquired absorption coefficient spectrum of each Bupleurum sample to obtain a quantized spectrum of each Bupleurum sample;

[0087] In this embodiment, specifically, the spectral quantization process is performed on the absorption coefficient spectra of four different varieties of Bupleurum radicis in the spectral range of 0.3 THz to 2 THz, and the quantization is carried out in the "Chinese medicine spectral quantum fingerprint consistency digital evaluation system 4.0". Quantization processing, the schematic diagram of the quantization processing process is shown in Figure 9 As shown, the specific operation of qua...

Embodiment 3

[0095] This embodiment provides a method for identifying the authenticity of Bupleurum radiata based on terahertz spectroscopy, which is used to identify the authenticity of Bupleurum radix unknown to be tested.

[0096] Specifically include the following steps:

[0097] Prepare a number of Bupleurum bupleurum samples to be tested and a genuine Bupleurum reference product. The specific sample preparation steps are the same as in Example 1 or 2. In this embodiment, Bei Bupleurum can be used as the genuine Bupleurum reference product;

[0098] Through the steps in Example 1 or 2, obtain the principal component score diagram of the Bupleurum bupleurum sample to be tested and the reference product of Bupleurum bupleurum;

[0099] Obtain the distribution results of the Bupleurum sample to be tested and the genuine Bupleurum reference product on the principal component score map through the obtained principal component score maps of the Bupleurum sample to be tested and the referenc...

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Abstract

The invention discloses a method for identifying the variety and authenticity of bupleurum chinense based on a terahertz spectrum technology. The method comprises the following steps: preparing a plurality of bupleurum chinense samples of known varieties; performing scanning determination on each prepared radix bupleuri sample through a terahertz time-domain spectrometer to obtain an absorption coefficient spectrum of each radix bupleuri sample; performing principal component analysis on the obtained absorption coefficient spectrum of each radix bupleuri sample to obtain a principal component score chart of each radix bupleuri sample; and obtaining a distribution result of each bupleurum sample on the principal component score plot through the obtained principal component score plot of each bupleurum sample. The method is based on the terahertz spectrum technology and is combined with a principal component analysis method, the absorption coefficient spectrums and principal component score diagrams of different radix bupleuri samples are obtained, complex pretreatment work is not needed, the variety and authenticity of radix bupleuri are accurately and efficiently identified, the method is convenient, rapid, scientific and accurate, and the working difficulty and subjective factor influence of radix bupleuri identification are effectively reduced.

Description

technical field [0001] The invention relates to the field of rapid detection, and more specifically, relates to a method for identifying Bupleurum species and their authenticity based on terahertz spectroscopy. Background technique [0002] Bupleurum is the dried root of Bupleurum or Bupleurum angustifolia. At present, the genuine Bupleurum recorded in the "Chinese Pharmacopoeia" mainly includes "Northern Bupleurum" and "South Bupleurum", which have the characteristics of reconciliation and retreat. Heat, soothes the liver, relieves stagnation, and elevates the central qi. It can be used to treat colds and fever, alternating cold and heat, malaria, liver depression and qi stagnation, sternocostal pain, prolapse of the anus, uterine prolapse, and irregular menstruation. It is widely used in clinical practice. However, due to the increasing clinical use of Bupleurum and low yield, authentic Bupleurum often cannot meet the needs of the market, resulting in a variety of counterf...

Claims

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Application Information

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IPC IPC(8): G01N21/3586
CPCG01N21/3586
Inventor 李茜李晓辉黄玉青曾志坚
Owner 太赫兹科技应用(广东)有限公司
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