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Application of pepper in preparation of medicine for treating and/or preventing respiratory virus pneumonia

A respiratory and anti-respiratory technology, applied in the field of biomedicine, can solve problems such as the effect and mechanism that have not yet been reported, and achieve the effect of good clinical application prospects.

Active Publication Date: 2022-03-15
SICHUAN CENT FOR TRANSLATIONAL MEDICINE OF TRADITIONAL CHINESE MEDICINE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In recent years, studies have found that Xanthanine has antispasmodic, anti-viral, anti-bacterial and fungal effects. However, the effect and mechanism of Xanthoxylum on respiratory viruses have not been reported yet.

Method used

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  • Application of pepper in preparation of medicine for treating and/or preventing respiratory virus pneumonia
  • Application of pepper in preparation of medicine for treating and/or preventing respiratory virus pneumonia
  • Application of pepper in preparation of medicine for treating and/or preventing respiratory virus pneumonia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1, the preparation of the medicine of the present invention containing Xanthophylline

[0027] Xanthophylline was formulated into solutions with concentrations of 6.25 μM, 12.5 μM, 25 μM, 50 μM, 100 μM and 200 μM.

experiment example 1

[0028] Experimental example 1, cytotoxicity experiment

[0029] Cell recovery: Take out the frozen 16HBE cells from the liquid nitrogen tank, quickly put them in a 37°C water bath and shake to dissolve them, add the dissolved cells to the 15ml BD tube that has absorbed 3ml of culture medium, centrifuge at 800rpm for 4min, discard The supernatant was resuspended by adding 1ml of culture medium, and the resuspended cells were added to a T25 culture flask and cultured in an incubator.

[0030] Cell plating: When the 16HBE cells were cultured to nearly 80%, digested, resuspended cells and counted, the diluted cell concentration was 1*10 5 / mL; 100 μL per well was added to a 96-well plate; the cells were dosed with different concentrations (6.25 μM, 12.5 μM, 25 μM, 50 μM, 100 μM, 200 μM) of prickly ash solution; set control wells and blank wells; put them in an incubator for overnight culture 24h. Aspirate the drug-containing culture medium, wash it three times with PBS, add new ...

experiment example 2

[0036] Experimental Example 2, Inhibition of Virus Activity Experiment

[0037] Digest and resuspend the cells in the logarithmic growth phase the day before the experiment, and mix 2*10 5 16HBE cells per well were seeded in 24-well plates and grown overnight. The next day, when the cell confluency reached 70-80%, add 200 μL of HMPV virus solution (MOI=10), and infect in the incubator for 2 hours; absorb the virus and wash it three times with PBS, prepare 200 μM Xanthomine mother solution, and use 3% FBS The DMEM culture solution was diluted into different concentrations of drug-containing solutions (0 μM, 12.5 μM, 25 μM, 50 μM, 100 μM, 200 μM), and the corresponding drug-containing solutions were added to each well of the experimental group. After 24 hours of action, the supernatant was discarded, RNA was extracted using a total RNA extraction kit, and RNA was extracted according to the instructions. Using the reverse transcription kit (AG11706-S), the extracted RNA was rev...

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PUM

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Abstract

The invention provides application of pepper in preparation of a medicine for treating and / or preventing respiratory virus pneumonia. The invention also discloses a method for effectively inhibiting and / or treating coronavirus, human metapneumovirus, influenza virus, parainfluenza virus, respiratory syncytial virus, measles virus, parotitis virus, rubella virus, adenovirus, cytomegalovirus, coxsackie virus, echovirus, herpes simplex virus, herpes zoster virus, herpes zoster virus, herpes zoster virus, herpes zoster virus, herpes zoster virus, herpes zoster virus, herpes zoster virus, herpes zoster virus and herpes zoster virus. The invention provides a new choice for clinical treatment of infection of respiratory viruses such as chicken pox-herpes zoster virus or rhinovirus.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to the use of Zanthoxylum bungeanum in the preparation of medicines for treating and / or preventing respiratory virus pneumonia. Background technique [0002] Respiratory virus is a virus that invades the respiratory tract and proliferates in the epithelial cells of the respiratory tract mucosa, causing local infection of the respiratory tract or lesions of tissues and organs outside the respiratory tract. It invades the lower respiratory tract and causes pulmonary infectious diseases, that is, respiratory virus pneumonia. Common respiratory viruses include influenza virus, parainfluenza virus, cytomegalovirus, adenovirus, rhinovirus, coronavirus, and certain enteroviruses, such as coxsackie, echovirus, etc., as well as herpes simplex, chickenpox- Herpes zoster, rubella, measles and other viruses, infants and young children are often infected with respiratory syncytial virus to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/436A61K36/758A61P11/00A61P31/16A61P31/14A61P31/22
CPCA61K31/436A61K36/758A61P11/00A61P31/16A61P31/14A61P31/22
Inventor 赵军宁华桦杨安东张翼冠戴瑛李金花
Owner SICHUAN CENT FOR TRANSLATIONAL MEDICINE OF TRADITIONAL CHINESE MEDICINE
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