Method for preparing hemostatic sponge from oxidized bletilla striata modified collagen fibers

Abstract

The invention discloses a method for preparing hemostatic sponge from oxidized bletilla striata modified collagen fibers. The preparation method comprises the following steps: oxidizing bletilla striata polysaccharide by adopting sodium periodate to prepare dialdehyde bletilla striata polysaccharide containing cross-linked active aldehyde groups, and chemically modifying collagenous fibers by taking the dialdehyde bletilla striata polysaccharide as a functional cross-linking agent. In the cross-linking process, the three-strand spiral structure of collagen is not damaged, and the porous three-dimensional network structure brings excellent filling power and water absorption performance. The blood coagulation activity of bletilla striata polysaccharide in the hemostatic sponge is well maintained, the platelet adhesiveness is remarkably improved, various blood coagulation indexes are superior to those of most of similar products, and the hemostatic performance is excellent. Meanwhile, the hemostatic sponge has excellent stability, the hemolysis rate meets the national safety requirement, the cytocompatibility is good, and the hemostatic sponge has the effects of promoting tissue growth, promoting wound healing and the like, and is an excellent novel hemostatic material.

Description

technical field [0001] The invention relates to a method for preparing a hemostatic sponge by oxidizing bletilla striata modified collagen fibers, which belongs to the field of biomedical materials. Background technique [0002] Wound bleeding caused by emergency accidents such as car accidents and wars in various occasions is often difficult to control. Bleeding from wounds is also considered the leading cause of death at the scene. According to multidisciplinary statistics, about 39% of deaths in accidents are caused by bleeding, most of which are due to hemorrhagic shock. Therefore, rapid and effective hemostasis of the wounded is extremely important, and shortening the hemostasis time of materials and improving the quality of hemostasis have become the best strategies to reduce the mortality of patients. In-depth research on various hemostatic materials has been widely carried out, among which collagen stands out due to its superior performance, and has gradually attra...

AI Technical Summary

Problems solved by technology

However, directly mixing Bletilla striata polysaccharides with collagen has the disadvantages of poor combination and easy loss

[0006] In order to overcome the shortcomings of poor collagen degradation resistance and long hemostasis time, etc., and at the same time break through the defects of poor mutual binding ability between Bletilla striata and collagen, the present invention, under the premise of keeping most of the sugar units of Bletilla striata polysaccharides intact, part of the sugar rings in Bletilla striata polysaccharides Oxidized to a structure with chemically active aldehyde groups, thereby preparing oxidized Bletilla striata polysaccharides with both hemostatic activity and chemical crosslinking activity