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Cerebral infarction early diagnosis marker, screening method and application, and construction method and application of cerebral infarction early diagnosis model

A technology of diagnostic markers and diagnostic models, applied in the field of diagnostic markers of cerebral infarction, can solve the problems of undetectable population, low specificity, poor sensitivity, etc., achieve high sensitivity, increase survival rate, and improve prognosis

Active Publication Date: 2022-03-04
CAPITAL NORMAL UNIVERSITY
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Problems solved by technology

[0007] For this reason, the technical problem to be solved by the present invention is to provide a diagnostic marker for cerebral infarction and its application to solve the sensitivity problem caused by the lack of effective integration of metabolomics and lipidomics in the current early diagnostic markers for cerebral infarction. The problem of poor specificity and low specificity can also solve some diagnostic methods, such as imaging diagnostic methods, which will limit some groups of people and cannot be checked; the present invention also provides a serum metabolomics and lipidomics based on UPLC-MS technology The screening method and application of diagnostic markers of cerebral infarction based on science, the method has good accuracy and high sensitivity for early diagnosis of cerebral infarction

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  • Cerebral infarction early diagnosis marker, screening method and application, and construction method and application of cerebral infarction early diagnosis model

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Embodiment Construction

[0060] The present invention is further explained below in conjunction with specific implementation examples, and the embodiments of the present invention are only used to explain the present invention, and do not limit the present invention in any form.

[0061] Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in this technical field, and the reagents and materials used are all commercially available.

[0062] 1. Collection and pretreatment of serum samples

[0063] 1.1 Collection of samples

[0064] A total of 105 serum samples were collected from a hospital, 45 samples from the cerebral infarction group (CON) and 60 samples from healthy people (CI) were used as the control group.

[0065] 1.2 Sample preprocessing

[0066] Metabolomics sample pretreatment: thaw serum samples at room temperature, take 100 microliters of serum in a 1.5mL centrifuge tube, add 400 microliters of aceto...

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Abstract

The invention discloses a cerebral infarction early diagnosis marker, a screening method and application, and a construction method and application of a cerebral infarction early diagnosis model. The diagnostic marker is composed of 4-dimethylallyltryptophan, taurochenodeoxycholic acid-3-sulfate, tri-hexose ceramide (d18: 1 / 18: 0), lysophosphatidylcholine (18: 0), arginine-alanine, asparaginic acid-tryptophan, methionine-arginine, sphingomyelin d37: 5, phosphatidyl glycerol (12: 0 / 21: 0) and glucosylceramide (d18: 0 / 18: 0). The diagnostic marker is screened out by simultaneously carrying out metabonomics analysis and lipidomics analysis on serum by utilizing a UPLC-MS (Ultra Performance Liquid Chromatography-Mass Spectrometry) technology; the sensitivity, the specificity, the accuracy and the AUC of the method for constructing the diagnosis model by utilizing the diagnosis marker are all greater than 0.9, and the method can be used for accurately distinguishing cerebral infarction patients from healthy people and can be used for early diagnosis of cerebral infarction.

Description

technical field [0001] The present invention relates to the technical field of cerebral infarction diagnostic markers. Specifically, early diagnosis markers, screening methods and applications of cerebral infarction, and construction methods and applications of early diagnosis models of cerebral infarction. Background technique [0002] Cerebral infarction (CI), also known as ischemic stroke, is a neurological disease that refers to the ischemic necrosis of local brain tissue caused by cerebral ischemia, hypoxia and blood supply disorders. The clinical symptoms of patients with cerebral infarction are manifested in aspects of consciousness, cognition, movement, language and feeling. Patients with mild disease may experience numbness and weakness of limbs and even dizziness in a short time; while those with severe disease are prone to hemiplegia and brain herniation. even death. Cerebral infarction has the characteristics of high morbidity, high mortality and low prevention...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/92G01N30/88G16B40/00G16B40/20G16B50/30G16B15/00G16H50/20
CPCG01N33/6893G01N33/92G01N30/88G16B40/00G16B40/20G16B50/30G16B15/00G16H50/20G01N2800/2871G01N2405/08G01N2405/04G01N2405/00G01N2410/00G01N2030/8813
Inventor 李中峰叶鑫鑫王英锋陈阳王丹
Owner CAPITAL NORMAL UNIVERSITY
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