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Bacillus velezensis capable of producing high-yield compound enzyme and efficiently degrading mycotoxin and application of bacillus velezensis

A technology of bacillus and bacteria agent, applied in the field of agricultural biology

Pending Publication Date: 2022-03-04
TIANJIN BIOFEED TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the research of this strain on the degradation of mycotoxins

Method used

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  • Bacillus velezensis capable of producing high-yield compound enzyme and efficiently degrading mycotoxin and application of bacillus velezensis
  • Bacillus velezensis capable of producing high-yield compound enzyme and efficiently degrading mycotoxin and application of bacillus velezensis
  • Bacillus velezensis capable of producing high-yield compound enzyme and efficiently degrading mycotoxin and application of bacillus velezensis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] Example 1, the screening and identification of Bacillus LB-Y-1

[0074] 1. Screening of strain LB-Y-1

[0075] 1. Primary screening of strains

[0076] Thirty-five cases of digesta samples from the rumen of dairy cows, chicken cecum, pig ileum, and rabbit cecum that were healthy and in good growth condition were collected for screening the target strains. The specific method is: take 10 g of each of the above samples, dissolve them in 90 mL of sterile PBS solution, shake at a constant temperature of 160 rpm for 20 minutes, absorb 500 μL and transfer them into 5.5 mL of NB liquid medium, and incubate at a constant temperature of 160 rpm at 37 °C for 24 hours, and then Take 100 μL of bacterial liquid, inoculate in 5.5 mL of Hormisch liquid medium, gradually increase the content of coumarin from 1 to 3 mg / mL, and culture at 37°C and 160 rpm for 48 hours to enrich the strains. After 5 times of enrichment, the culture solution was diluted and spread on NB solid medium (dil...

Embodiment 2

[0096] Embodiment 2, the ability of Bacillus velesi LB-Y-1 to produce protease, cellulase and amylase

[0097] 1. Protease-producing ability of Bacillus veleisi LB-Y-1

[0098] 1. Draw the L-tyrosine standard curve: pre-configure 0.1mol / L HCL solution as the solvent, and use 10μg / mL as the gradient difference to prepare a tyrosine standard solution with a concentration of 0-70μg / mL. Take 1mL of the above standard solution and 5mL of 0.4mol / L Na 2 CO 3 Mix the solution with 1mL of Folon-Phenol reagent, and measure the absorbance at the wavelength of 680nm with a UV spectrophotometer after bathing in water at 40°C for 20min, and draw the OD of L-tyrosine 680 The relationship between concentration and concentration.

[0099] 2. Prepare the seed solution according to the method in Example 1, inoculate the seed solution in fermentation medium A, and culture at 37° C. and 160 rpm / min for 36 hours.

[0100] 3. After completing step 2, centrifuge at 4°C and 8000 rpm for 15 minutes...

Embodiment 3

[0123] Example 3, the effect of Bacillus velei LB-Y-1 on the growth performance of broilers and the diversity of intestinal microorganisms

[0124] 1. Feed test of broiler chickens with different gradients of Bacillus LB-Y-1

[0125] Select 300 1-day-old healthy Aiba Yijia broiler chickens (AA broilers), and randomly divide them into 5 treatment groups, each treatment group has 6 replicates, and each replicate has 10 chickens, and each group is the control group CON, shellfish Bacillus lesii group BV1, BV2, BV3 and antibiotic AGPs group. The CON group was fed with basal diet; BV1, 2, and 3 groups were sprayed with Bacillus Veles suspension in the basal diet, so that the bacterial concentration in the basal diet was 1.0×10 7 CFU / kg, 1.0×10 8 CFU / kg, 1.0×10 9 CFU / kg; AGPs group added combination antibiotics (chlortetracycline 100mg / kg, kitasamycin 20mg / kg) in the basal diet. The basal ration was a corn-soybean meal ration, and the formula design was based on the "Chicken Feedi...

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Abstract

The invention discloses bacillus velezensis capable of producing high-yield compound enzyme and efficiently degrading mycotoxin and application of the bacillus velezensis. The bacillus velezensis is bacillus velezensis LB-Y-1, and the bacillus velezensis LB-Y-1 not only can effectively degrade AFB1, but also has the capability of producing protease, cellulase and amylase at high yield. The broiler chicken in-vivo test shows that the strain has the potential probiotic characteristics of high safety, strong stress resistance, easiness in enteral colonization, improvement of intestinal flora structure, improvement of growth performance and the like. In addition, the strain can degrade AFB1 in mildewed peanut meal through fermentation treatment, the content of acid soluble protein is increased, and biological detoxification and quality improvement of the peanut meal are achieved. The comprehensive effects of eliminating and inhibiting mycotoxin pollution in the feed and the raw materials, reducing the crude fiber content of the raw materials, increasing the small peptide content and the like are achieved.

Description

technical field [0001] The invention relates to the field of agricultural biotechnology, in particular to a strain of Bacillus velezensis (Bacillus velezensis) and its application, in particular to a strain of Bacillus velezensis (Bacillus velezensis) LB-Y- which can efficiently degrade mycotoxins at the same time with a high-yield compound enzyme. 1 and its application. Background technique [0002] Mycotoxins are secondary metabolites produced by fungi. They are ubiquitous and unavoidable contaminants in food and feed. Consumption of mycotoxins by humans and animals can lead to illness and death. Aspergillus, Penicillium and Fusarium species are all capable of producing a variety of mycotoxins. For example, Aspergillus flavus, Aspergillus parasiticus, Aspergillus knossii and Aspergillus pseudoaflatus are the main culprits for the production of a series of highly toxic substances aflatoxin. Aflatoxin B since 1960 1 (Aflatoxin B 1 , AFB 1 ) has been verified to be highl...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02A23L33/135A23K10/18A23K50/75A23K10/37A23L5/20C12R1/07
CPCA23L33/135A23K10/18A23K50/75A23K10/37A23L5/28A23V2002/00A23V2200/3204Y02P60/87
Inventor 邓雪娟李冲蔡辉益李爽李淑珍王腾飞
Owner TIANJIN BIOFEED TECH CO LTD
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