Whitening collagen peptide freeze-dried powder and preparation method thereof
A technology of white collagen and freeze-dried powder, which is applied in the preparation method of peptide, freeze-dried transportation, peptide/protein composition, etc., can solve the problem of lack of whitening collagen peptide products, and achieve easy operation, high whitening activity, and advanced technology simple effect
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Embodiment 1
[0024] A preparation method of whitening collagen peptide freeze-dried powder, adopts the following steps:
[0025] (1) Raw material preparation: add 100g of pigskin collagen into water, adjust the pH to 3.0 with acetic acid and stir to prepare a solution;
[0026] (2) One-time enzymatic hydrolysis: add 0.15g pepsin and 0.15g papain to the solution in step (1), adjust the pH to 1.5 and stir, control the temperature at 30°C for 0.5 hours, inactivate the enzyme, and adjust the pH to neutral ;
[0027] (3) Membrane separation: the enzymolysis solution obtained in step (2) is ultrafiltration treated with an ultrafiltration membrane with a molecular weight cut-off of 1500Da, and the retentate is collected;
[0028] (4) Secondary enzymatic hydrolysis: add 0.75 g of chymotrypsin complex protease and 0.25 g of alkaline protease to the retentate obtained in step (3), adjust the pH to 7.5 and stir, and enzymolyze at a temperature of 30° C. for 1 hour to inactivate the enzyme;
[0029]...
Embodiment 2
[0032] A preparation method of whitening collagen peptide freeze-dried powder, adopts the following steps:
[0033] (1) Raw material preparation: add 100g of bovine bone collagen into water, adjust the pH to 4.0 with acetic acid and stir to prepare a solution;
[0034] (2) One-time enzymatic hydrolysis: add 0.2g pepsin and 0.4g papain to the solution in step (1), adjust the pH to 2.5 and stir, control the temperature at 40°C for 2 hours, inactivate the enzyme, and adjust the pH to neutral ;
[0035] (3) Membrane separation: the enzymolysis solution obtained in step (2) is ultrafiltration treated with an ultrafiltration membrane with a molecular weight cut-off of 1500Da, and the retentate is collected;
[0036] (4) Secondary enzymatic hydrolysis: add 1.5 g of chymotrypsin complex protease and 0.5 g of alkaline protease to the retentate obtained in step (3), adjust the pH to 8.5 and stir, and enzymolyze at a temperature of 40° C. for 3 hours to inactivate the enzyme;
[0037] ...
Embodiment 3
[0040] A preparation method of whitening collagen peptide freeze-dried powder, adopts the following steps:
[0041] (1) Raw material preparation: add 100g of fish scale collagen into water, adjust the pH to 3.5 with acetic acid and stir to prepare the solution;
[0042] (2) Enzymolysis once: add 0.35g pepsin and 0.35g papain to the solution in step (1), adjust the pH to 2.0 and stir, control the temperature at 35°C for 1 hour, inactivate the enzyme, and adjust the pH to neutral ;
[0043] (3) Membrane separation: the enzymolysis solution obtained in step (2) is ultrafiltration treated with an ultrafiltration membrane with a molecular weight cut-off of 1500Da, and the retentate is collected;
[0044] (4) Secondary enzymatic hydrolysis: add 1.0 g of chymotrypsin complex protease and 0.5 g of alkaline protease to the retentate obtained in step (3), adjust the pH to 8.0 and stir, and enzymolyze at a temperature of 35° C. for 2 hours to inactivate the enzyme;
[0045] (5) Vacuum ...
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