A non-irritating essence with whitening and moisturizing functions and preparation method thereof
A technology of moisturizing function and essence, applied in medical preparations containing active ingredients, preparations for skin care, pharmaceutical formulas, etc., can solve problems such as unreported
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Embodiment 1
[0029] Embodiment 1, the preparation of Brassica chinensis ethanol extract
[0030] Take the root of Baizicai as raw material, wash it, crush it into a powder with a particle size of 40 mesh, add 80% ethanol solution of 8 times the total amount of the powder, heat and reflux for extraction twice, each time for 1.5h, and the extraction temperature is 70°C , Filtrate, combine the filtrates, remove ethanol under reduced pressure, and concentrate.
[0031] According to the above method, 75% and 65% ethanol solutions are used for extraction to obtain 75% ethanol extract and 65% ethanol extract respectively.
Embodiment 2
[0032] Embodiment two, the preparation of water extract of Chinese cabbage
[0033] Take the root of Baizicai as raw material, wash it, crush it into a powder with a particle size of 40 mesh, add 8 times the total amount of the coarse powder, and extract it twice with hot water at a temperature of 80°C, 6 hours each time, filter, combine the filtrate, and concentrate , that is.
Embodiment 3
[0034] Example 3. Comparison of Whitening Activity and Stimulus of Alcoholic Extract and Water Extract of Brassica chinensis
[0035] 3.1 Comparison of whitening activity
[0036] Each group of samples was diluted with disodium hydrogen phosphate-citric acid buffer solution (pH6.8, 0.05mol / L) to obtain multi-level concentration samples, and samples of each concentration were taken in glass test tubes, and tyrosinase solution was added respectively; at 30°C After reacting in a water bath for 10 minutes, add levodopa solution to each group of glass test tubes in turn, then react in a water bath at 30°C for 10 minutes, and measure the OD value at 475 nm. The experimental system is shown in Table 1.
[0037]
[0038] In the formula: T is the absorbance value of the sample tube, that is, the absorbance value of the solution after the sample reacts with tyrosinase; T 0 is the background absorbance value of the sample; C is the average value of the three times of the absorbance v...
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