Method for constructing characteristic chromatograms of dried orange peel medicinal material and preparation

A characteristic map and construction method technology, applied in the field of feature map construction, can solve the problems of inability to comprehensively evaluate the quality of tangerine peel medicinal materials and their preparations, and achieve effective and reliable product quality and good separation effects

Pending Publication Date: 2022-03-01
YAAN THREE NINE PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for constructing characteristic maps of tangerine peel medicinal materials and preparations, so as to solve the quality technical problems that cannot fully evaluate tangerine peel medicinal materials and preparations thereof in the prior art

Method used

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  • Method for constructing characteristic chromatograms of dried orange peel medicinal material and preparation
  • Method for constructing characteristic chromatograms of dried orange peel medicinal material and preparation
  • Method for constructing characteristic chromatograms of dried orange peel medicinal material and preparation

Examples

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preparation example Construction

[0073] In specific embodiments of the present invention, the preparation method of described need testing solution comprises:

[0074] When the substance to be tested is tangerine peel medicinal material or tangerine peel preparation in solid or semi-solid dosage form, after ultrasonic extraction of the tangerine peel medicinal material or preparation with methanol as the extraction solvent, the lost weight is supplemented by the extraction solvent, filtered, and taken Continue filtrate as need testing solution;

[0075] When the substance to be tested is a tangerine peel preparation in a liquid dosage form, the liquid can be directly taken as the test solution for analysis.

[0076] In a specific embodiment of the present invention, the preparation is a single flavor preparation of tangerine peel. Further, the single flavor preparation of orange peel is formula granules of orange peel.

[0077] In a specific embodiment of the present invention, when the substance to be test...

Embodiment 1

[0096] This embodiment provides a method for constructing a characteristic map of tangerine peel medicinal materials, including the following steps:

[0097] (1) Preparation of test solution

[0098] Accurately weigh 0.2g of dried tangerine peel medicinal powder (through No. 2 sieve), put it in a stoppered Erlenmeyer flask, accurately add 25mL of methanol, seal it tightly, weigh it, ultrasonically treat it (power 300W, frequency 40kHz) for 45min, let it cool, Weigh again, make up the lost weight with methanol, shake well, filter, and take the filtrate to obtain the test solution.

[0099] (2) Preparation of reference substance solution

[0100] Accurately weigh the appropriate amount of naringel rutin reference substance, hesperidin reference substance, nobiletin reference substance and tangeretin reference substance, add methanol to make a mixed solution containing 100 μg of the reference substance per 1 mL, and obtain the reference substance solution.

[0101] (3) Detectio...

Embodiment 2

[0108] This example provides a method for constructing a characteristic map of orange peel formula granules, specifically refer to the method in Example 1, the only difference is that the preparation method of the test solution in step (1) is different.

[0109] The preparation method of the need testing solution of the present embodiment comprises:

[0110] Take an appropriate amount of tangerine peel formula granules, grind finely, accurately weigh 0.2g, place in a stoppered Erlenmeyer flask, accurately add 50mL of methanol, weigh, ultrasonically treat (power 300W, frequency 40kHz) for 30min, let cool, and weigh again , Use methanol to make up the lost weight, shake well, filter, take the continued filtrate, that is, the test solution.

[0111] Such as figure 2 Shown is the characteristic spectrum of tangerine peel formula granules (two batches). Among them, 1 # The peak corresponds to naringerin rutin, 2 # The peak is the reference peak, corresponding to hesperidin, 5 ...

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Abstract

The invention relates to the technical field of quality analysis and control of medicinal materials and preparations thereof, in particular to a construction method of a characteristic chromatogram of a pericarpium citri reticulatae medicinal material and a preparation thereof. The construction method of the characteristic chromatogram of the pericarpium citri reticulatae medicinal material and the preparation comprises the following steps: detecting a test solution by adopting an HPLC (High Performance Liquid Chromatography) method to obtain the characteristic chromatogram; the detection conditions of the HPLC method are as follows: acetonitrile is taken as a mobile phase A, and water is taken as a mobile phase B for gradient elution. According to the invention, the method for determining the characteristic chromatogram of the dried orange peel medicinal material and the preparation is established, and good separation of chromatographic peaks is realized; according to the construction method of the characteristic chromatogram of the pericarpium citri reticulatae medicinal material and the preparation, the obtained characteristic chromatogram contains 8 characteristic peaks of the pericarpium citri reticulatae medicinal material and 7 characteristic peaks of the pericarpium citri reticulatae preparation such as pericarpium citri reticulatae formula granules, so that main components of the pericarpium citri reticulatae medicinal material or the preparation can be comprehensively represented; the method has very important significance for comprehensively evaluating the quality of the dried orange peel medicinal material or preparation.

Description

technical field [0001] The invention relates to the technical field of quality analysis and control of medicinal materials and preparations thereof, in particular to a method for constructing characteristic maps of dried tangerine peel medicinal materials and preparations. Background technique [0002] Chenpi is the dry ripe pericarp of Rutaceae Citrus reticulata Blanco and its cultivars. It has the effects of regulating qi and reducing adverse events, regulating the middle and appetizing, drying dampness and resolving phlegm. At this stage, in the 2020 edition of the Pharmacopoeia of the People's Republic of China, the content of hesperidin is only controlled for tangerine peel, and no HPLC characteristic map has been established for the medicinal material, which cannot achieve the purpose of comprehensive quality evaluation of the medicinal material and its preparations. [0003] The characteristic map of traditional Chinese medicine refers to the chromatographic or spect...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/86
CPCG01N30/8686
Inventor 韦婷侯新莲史琳莉
Owner YAAN THREE NINE PHARMA
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