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Intelligent sampling and detecting device suitable for microorganism suspension liquid and solid culture medium

A solid culture medium and detection device technology, applied in sampling devices, analysis materials, instruments, etc., can solve the problems of sample waste, inability to guarantee aseptic operation, difficulty in sampling and detection at the same time, etc., to eliminate the probability of bacterial contamination, Good sterilization effect, guaranteed drying effect

Pending Publication Date: 2022-03-01
CHANGCHUN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Due to the limited detection technical conditions, it is difficult to carry out sampling and detection of microbial suspension and solid medium at the same time. The sampling process basically relies on manual labor, and mechanical automation is rarely used for sampling. After manual sampling, sample testing needs to be carried out in the laboratory, which cannot guarantee strict If the aseptic operation is carried out, the sample will be wasted if it is contaminated; the manual sampling and testing process has limitations, and it is difficult to guarantee the efficiency and accuracy of sampling

Method used

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  • Intelligent sampling and detecting device suitable for microorganism suspension liquid and solid culture medium
  • Intelligent sampling and detecting device suitable for microorganism suspension liquid and solid culture medium
  • Intelligent sampling and detecting device suitable for microorganism suspension liquid and solid culture medium

Examples

Experimental program
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Effect test

Embodiment 2

[0034] The specific implementation of getting Mortierella Ramanii spores from flat petri dish:

[0035] When the self-adaptive sampling brush head (13) is perpendicular to the direction of the multi-region sample tray (7), it is used for sampling spores in a solid plate culture dish, and the self-adaptive sampling brush head (13) is kept in contact with the spore colony for 3 seconds to complete the sampling Afterwards, the self-adaptive sampling brush head (13) with spores adsorbed is transferred to the dilution system to dilute the spore suspension. Sterilize the multi-area sample tray with absolute ethanol, set the sampling period to 0-8 days, and the sampling time is 10:00 every day. When the adaptive sampling brush head is perpendicular to the direction of the multi-area sample tray, it is used for solid The spores in the flat petri dish are sampled, and the intelligent detection device checks whether the Mortierella Ramanii flat petri dish is contaminated with bacteria. ...

Embodiment 3

[0037] Get the specific embodiment of throwing sporulation yeast from slant culture medium:

[0038] Use absolute ethanol to sterilize the multi-area sample tray, set the sampling period to 0-5 days, and the sampling time is 10:00 every day, place the slant medium at an angle of 45° on the multi-area sample tray, under the slant medium The height from the side to the multi-area sample tray is 5cm. The intelligent detection device checks whether the culture medium of the sporulation yeast slant is contaminated with bacteria. If the bacteria is contaminated, it is sent to the three-dimensional sterilization chamber for sterilization. The sampling point is set according to 45°, and the mechanical arm controls the sampling needle to tilt 45° to be parallel to the direction of the test tube; Adapt to the sampling brush head and send it to the dilution system to dilute with physiological saline to make a suspension of sporozoan yeast and send it to the sample detection system for bi...

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Abstract

The invention discloses an intelligent sampling and detecting device suitable for microorganism suspension liquid and a solid culture medium. The device comprises an intelligent sample detecting system, a three-dimensional sterilizing bin, a multi-dimensional sampling period controller, a synchronous sampling controller, an image collecting system, a strain recognizing device, a sample diluting system and an information storing system. The method comprises the following steps: detecting whether a sample is contaminated by bacteria or not through a sample intelligent detection system, identifying a strain of a non-contaminated sample, performing image acquisition, sampling according to a set sampling period and sampling time, diluting the sample according to detection requirements, detecting the sample, and finally storing all data into an information storage system; according to the invention, traditional manual sampling can be replaced, the bacterial contamination rate in the detection process is reduced, a strain recognition system can lock a target system and eliminate infectious microbes, and the probability of bacterial contamination in the fermentation culture process is more favorably eliminated.

Description

technical field [0001] The invention relates to the field of microbial fermentation, in particular to an intelligent sampling and detection device suitable for microbial suspension and solid medium. Background technique [0002] Microbial fermentation and cultivation technology is a very important research direction at present. In the process of microbial fermentation and cultivation, it involves the growth and metabolism of bacteria, as well as the influence of different fermentation conditions on microbial fermentation. The parameters of the product are measured, and the measurement results are of great significance to the research results. [0003] Due to the limited detection technical conditions, it is difficult to carry out sampling and detection of microbial suspension and solid medium at the same time. The sampling process basically relies on manual labor, and mechanical automation is rarely used for sampling. After manual sampling, sample testing needs to be carried...

Claims

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Application Information

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IPC IPC(8): G01N1/14
CPCG01N1/14G01N35/10
Inventor 刘欣孔燕琴杨紫琪王赫
Owner CHANGCHUN UNIV OF TECH
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