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Stem cell film forming culture medium and application thereof

A stem cell and culture medium technology, applied in the direction of cell culture active agent, cell culture support/coating, animal cells, etc., can solve the problems of low homing rate and complicated treatment, and achieve high immunocompatibility and onset of effect Long-lasting, easy-to-achieve effects

Pending Publication Date: 2022-03-01
BINZHOU MEDICAL COLLEGE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve the problems of complex pre-transplantation treatment and low homing rate after transplantation when human umbilical cord mesenchymal stem cells (hUC-MSCs) treat premature ovarian failure (POF) in the prior art, and propose a stem cell Film-forming medium

Method used

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  • Stem cell film forming culture medium and application thereof
  • Stem cell film forming culture medium and application thereof
  • Stem cell film forming culture medium and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Preparation of medium: According to the instructions of DMEM / F12, pour a bag of medium into the container, wash the residual medium in the bag with a small amount of water for injection, incorporate it into the container, add water for injection (temperature 20-30 ° C) to 950ml, Slightly stir to dissolve, add sodium bicarbonate and penicillin, add water for injection to 1L after slight stirring to dissolve, adjust pH to 7.5 with 1mol / L sodium hydroxide solution or hydrochloric acid solution, add fetal bovine serum to make the final concentration 60μl / ml, add Epidermal growth factor to make the final concentration 45ng / ml, adding basic fibroblast growth factor to make the final concentration 35ng / ml, adding vitamin A to make the final concentration 0.5ng / ml, vitamin B1 to make the final concentration 1.2ng / ml, The final concentration of vitamin C was 16ng / ml, and the final concentration of vitamin E was 3ng / ml. After slight stirring to dissolve, sterilize through 0.22 μm ...

Embodiment 2

[0030] Culture medium preparation: According to the MEM instructions, add 950ml of ultrapure water to the container, pour a bag of medium into the container, wash off the residual medium in the bag with ultrapure water, put it into the container, stir to dissolve, and add bicarbonate Sodium, HEPES, penicillin, stirred slightly to dissolve, then added purified water to 1L, adjusted pH to 7.2 with 1mol / L sodium hydroxide solution or hydrochloric acid solution, added fetal bovine serum to make the final concentration 60μl / ml, added epidermal growth factor to make the final concentration The concentration is 35ng / ml, add basic fibroblast growth factor to make the final concentration 20ng / ml, add vitamin B1 to make the final concentration 2ng / ml, vitamin C to make the final concentration 25ng / ml, vitamin E to make the final concentration 5ng / ml, after stirring slightly to dissolve, pass through a 0.22μm membrane to sterilize, add sterile detoxified type III collagen and type IV col...

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Abstract

The invention relates to a stem cell film-forming culture medium and application thereof, the stem cell film-forming culture medium comprises a cell basal culture medium, fetal calf serum, growth factors, an attachment skeleton material and vitamins, and the method relates to preparation of the stem cell film-forming culture medium, separation of stem cells, culture of the stem cells and stem cell film formation. The method has the beneficial effects that the collagen is subjected to low-temperature centrifugal membrane forming by utilizing the dissolving property of the collagen, so that the operation cost and the technical requirements are low, and large-scale production is facilitated; the finally obtained stem cell biological patch is high in immunocompatibility, few in adverse reaction and thin in membrane thickness, the thickness can be controlled according to the temperature and the centrifugal time, the stem cells are embedded into the biological patch membrane, the stem cells are slowly released along with absorption of a matrix by a body after transplantation, and therefore the effect taking time is longer, and the survival rate of the stem cells is increased. Compared with a stem cell biological patch prepared by common culture and adsorption, the stem cell biological patch has a better ovary repairing effect.

Description

technical field [0001] The invention relates to the field of cell culture, in particular to a stem cell film-forming medium and application thereof. Background technique [0002] Premature ovarian failure (POF) refers to the phenomenon of amenorrhea before the age of 40 caused by ovarian failure. A series of symptoms of low estrogen, such as hot flashes and sweating, facial flushing, low libido, infertility, etc., seriously affect the health of women of childbearing age. Studies have confirmed that mesenchymal stem cells (MSCs) can regulate decidual immune cells, and the tail vein injection can repair the aging ovarian structure in mice and improve ovarian endocrine function, indicating that the use of mesenchymal stem cells (MSCs) to treat ovarian Progeria (POF) has potential clinical application value. However, the microenvironment at the site of ovarian injury will lose its normal compliance due to inflammatory response and the deposition of extracellular matrix. The re...

Claims

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Application Information

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IPC IPC(8): C12N5/0775
CPCC12N5/0668C12N2501/11C12N2501/115C12N2533/54C12N2500/38C12N2509/00C12N2509/10
Inventor 付强
Owner BINZHOU MEDICAL COLLEGE
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