Micro-fluidic chip device for biogenic amine detection and application

Abstract

The invention discloses a micro-fluidic chip device for biogenic amine detection and application. The micro-fluidic chip device comprises a sample inlet, a connecting channel, a detection module and an air pressure driving area; the sample inlet, the detection module and the air pressure driving area are sequentially connected through the connecting channel; the sample inlet is connected with the atmosphere; the detection module comprises a cellulose acetate-based fluorescent substance modified three-dimensional porous polydimethylsiloxane (PDMS) structure, and the air pressure driving area provides negative pressure in a pressing mode to promote to-be-detected gas or liquid outside the chip to enter the chip. The food freshness can be judged by detecting the response conditions of biogenic amines in different foods. The prepared micro-fluidic device has quick and reversible response to biogenic amine, so that the micro-fluidic device can be easily used as a quick detection device and can be practically applied to the food industry.

Description

technical field [0001] The invention relates to a microfluidic chip device and application for biogenic amine detection, belonging to the field of microfluidic chip biology. Background technique [0002] Biogenic amines (BA), such as histamine, tyramine, putrescine, and cadaverine, are a general term for a class of biologically active nitrogen-containing low-molecular-weight organic compounds. Most foods contain biogenic amines, which are mainly formed by the decarboxylation of amino acids or the transamination of aldehydes and ketones. Excessive intake can cause adverse physiological reactions such as headache, abdominal cramps, and vomiting. Therefore, monitoring the level of biogenic amines in food is very important to ensure food safety. [0003] Currently, there are many methods for detecting biogenic amines, including optical sensor method, thin layer chromatography (TLC), capillary electrophoresis (CE), gas chromatography-mass spectrometry (GC-MS) and high performanc...

AI Technical Summary

Problems solved by technology

[0004] Traditional optical sensors based on changes in the fluorescence intensity of a luminous body, its measurement accuracy is easily affected by the luminous body concentration, measuring instruments, and external environments (such as temperature, humidity, etc.)

And the single optical sensor is weak, which can easily lead to the failure of the naked eye detection mode

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