Hepatic failure detection reagent and application thereof in hepatic failure detection

A detection reagent and technology for liver failure, which is applied in the detection of liver failure based on the specific fingerprint of serum glycoprotein oligosaccharide chain detection, and in the field of detection of liver failure, can solve the problems of low repeatability, small injection volume and high sensitivity, etc. Achieve high specificity, high accuracy, high repeatability, and high sensitivity

Pending Publication Date: 2022-02-11
JIANGSU XIANSIDA BIOTECH CO LTD +1
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the mass spectrometry instrument is precise, the operation of the equipment is complicated, and the mass spectrometer is expensive, so it is not suitable for popularization and use in clinical practice; (3) capillary electrophoresis (CE...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Hepatic failure detection reagent and application thereof in hepatic failure detection
  • Hepatic failure detection reagent and application thereof in hepatic failure detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1 detects liver failure

[0050] Statistical analysis was carried out by measuring the G-Test specific fingerprint of serum glycoprotein oligosaccharide chains, and the materials and methods used:

[0051] 1. Test samples: collect serum from patients with liver failure and normal controls.

[0052] 2. Experimental equipment: sugar group analyzer, PCR, centrifuge.

[0053] 3. Reagent preparation:

[0054] Reagent A: prepared by adding SDS with a mass concentration of 0.5 to 5% in ammonium bicarbonate solution with a concentration of 10 mM;

[0055] Reagent B: It is prepared by mixing 0.01~10U / 10μL glucosamidase and 0.01~10U / 10μL sialidase, and the pH value of the mixed solution is 4~9;

[0056] Reagent C: Prepared by dissolving 8-aminopyrene-1,3,6-trisulfonic acid in DMSO, the concentration is 0.01mM~1M;

[0057] Reagent D: stop solution.

[0058] 4. Glycosequencing detection steps:

[0059] Step 1 Preparation of oligosaccharide chains

[0060] Add ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a hepatic failure detection reagent and a preparation method thereof, and the detection reagent is prepared by mixing the following reagents: a reagent A, which is prepared by adding 0.5-5% by mass of SDS into an ammonium bicarbonate solution with a concentration of 10 mM; a reagent B, which is prepared by mixing 0.01-10 U/10 [mu] L of glycosamine acylase and 0.01-10 U/10 [mu] L of sialidase, wherein the pH value of the mixed solution is 4-9; a reagent C, which is prepared by dissolving 8-aminopyrene-1, 3, 6-trisulfonic acid in DMSO (dimethylsulfoxide), and the concentration of the reagent C is 0.01 mM to 1M; and a reagent D: a stop solution. According to the invention, a glycome map in serum is determined by a detection reagent, and a peak value is quantified for statistical analysis, so that the establishment method of the hepatic failure serum glycome map model is provided for detecting hepatic failure.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and relates to a detection method for liver failure, in particular to a detection method for liver failure based on the specific fingerprint of serum glycoprotein oligosaccharide chain detection (G-Test). Background technique [0002] Liver failure (Liver Failure) refers to severe liver damage caused by a variety of factors, leading to serious impairment or decompensation of its synthesis, detoxification, excretion and biotransformation functions, resulting in coagulation mechanism disorders, jaundice, hepatic encephalopathy , ascites, etc. as the main manifestations of a group of clinical syndrome. Generally can be divided into acute liver failure (Acute Liver Failure, ALF) and chronic liver failure (Chronic Liver Failure, CLF). The clinical manifestations are extreme fatigue, and severe gastrointestinal symptoms such as obvious anorexia, abdominal distension, nausea, and vomiting. As one o...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N27/447G01N21/64G16H50/20G16H50/30G16H50/80
CPCG01N27/44726G01N21/6428G16H50/20G16H50/30G16H50/80G01N2021/6439
Inventor 陈翠英王蕾谈宗男徐蕾
Owner JIANGSU XIANSIDA BIOTECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap