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Detection kit for detecting aspirin metabolism ability and preparation method thereof

A detection kit and a technology of metabolic capacity, applied in the field of biochemical detection, can solve the problems of poor accuracy and high false positive rate, improve the capture efficiency and detection sensitivity, and reduce the failure or poor effect of anti-platelet aggregation therapy.

Active Publication Date: 2022-04-01
湖南菲思特精准医疗科技有限公司
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  • Abstract
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AI Technical Summary

Problems solved by technology

ELISA is currently still the gold standard for detecting aspirin metabolism, but the current ELISA has the problems of high false positive rate and poor accuracy

Method used

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  • Detection kit for detecting aspirin metabolism ability and preparation method thereof
  • Detection kit for detecting aspirin metabolism ability and preparation method thereof
  • Detection kit for detecting aspirin metabolism ability and preparation method thereof

Examples

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Embodiment Construction

[0035] The detection kit for detecting aspirin metabolism provided by the present invention and the preparation method thereof will be further described in detail and completely below in conjunction with the examples. The embodiments described below are exemplary only for explaining the present invention and should not be construed as limiting the present invention.

[0036] The experimental methods in the following examples are conventional methods unless otherwise specified. The experimental materials used in the following examples were purchased from the market unless otherwise specified.

[0037]The enzyme-linked immunosorbent assay method for the curative effect of aspirin disclosed in this embodiment is to detect the content of 11-DH-TXB2 in human urine by using enzyme-linked immunosorbent assay. The enzyme-linked immunosorbent assay in this example adopts the principle of the competition method, uses the enzyme-linked immunosorbent assay method and the enzyme-linked im...

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Abstract

The invention discloses a detection kit for detecting the metabolic capacity of aspirin and a preparation method thereof, wherein the kit includes a 11-dehydrothromboxane B2 monoclonal antibody-coated microtiter plate, a 11-dehydrothromboxane B2 Thromboxane B2 competitor, chromogenic substrate, stop solution and 11-dehydrothromboxane B2 standard solution, the monoclonal antibody of 11-dehydrothromboxane B2 is mouse monoclonal antibody, and the light chain variable region of mouse monoclonal antibody The sequence is shown in SEQ ID NO.2 of the sequence listing, and the sequence of the heavy chain variable region of the mouse monoclonal antibody is shown in SEQ ID NO.4 of the sequence listing. The invention adopts an enzyme-linked immunosorbent detection kit and its method to detect aspirin metabolism so as to judge whether a patient has aspirin resistance or resistance. It has the characteristics of accuracy and high sensitivity, and is not easily affected by biochemical indicators such as creatinine. Failure or ineffectiveness of antiplatelet aggregation therapy due to aspirin resistance.

Description

technical field [0001] The invention relates to a detection kit for detecting aspirin metabolism capacity and a preparation method thereof, belonging to the field of biochemical detection. Background technique [0002] 11-dehydro-thromboxane B2 (11-dehydro-thromboxaneB2, 11-DH-TXB2) is the terminal metabolite of thromboxane A2 (TXA2), which is mainly excreted through the kidneys, and is involved in hemostasis and the occurrence of cardiovascular diseases. Play an important role. Arachidonic acid generates prostaglandin H under the action of prostaglandin H synthase 1 and 2 (COX-1 and COX-2). Prostaglandin H is chemically unstable and can be converted into a variety of biologically active prostaglandins under the action of isomerase, including TXA2, prostacyclin I2, prostaglandin D2, prostaglandin E2, and prostaglandin F2α. TXA2 is mainly synthesized in platelets through the COX-1 pathway. Neonatal platelets express both COX-1 and COX-2, while mature platelets only express ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/577G01N33/58G01N33/543G01N33/88G01N21/78G01N21/31C07K16/44
CPCG01N33/577G01N33/581G01N33/54306G01N33/88G01N33/9486G01N21/78G01N21/31C07K16/44G01N2800/222G01N2021/786C07K2317/56
Inventor 刘丹易倩春
Owner 湖南菲思特精准医疗科技有限公司
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