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Lactobacillus fermentum for preventing and treating hyperuricemia as well as composition and application thereof

A technology of Lactobacillus fermentum and hyperuricemia, applied in the directions of Lactobacillus, application, drug combination, etc., can solve the problems of unassessed impact, poor targeting of probiotic strains, etc., achieve good safety, improve intestinal and kidney Structural damage and inflammatory response, broad-spectrum antibacterial effect

Active Publication Date: 2022-02-01
SICHUAN GAOFUJI BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the probiotic strains reported in the prior art for relieving hyperuricemia and gout are not highly targeted. At the same time, the impact of the corresponding strains on the structure of the human intestine and kidney has not been evaluated, and no postbiotics of the relevant strains have been shown to relieve hyperuricemia. Therefore, the development of new strains and their postbiotics for the preparation of a convenient and efficient drug or functional food for alleviating and treating hyperuricemia and gout is of great significance and great market value

Method used

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  • Lactobacillus fermentum for preventing and treating hyperuricemia as well as composition and application thereof
  • Lactobacillus fermentum for preventing and treating hyperuricemia as well as composition and application thereof
  • Lactobacillus fermentum for preventing and treating hyperuricemia as well as composition and application thereof

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Experimental program
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Effect test

Embodiment 1

[0063] A Lactobacillus fermentum GF1800 (Limosilactobacillusfermentum GF1800) for preventing and treating hyperuricemia of the present invention, preservation number: CCTCC NO: M 2021984.

[0064] 1) Isolation and screening of Lactobacillus fermentum GF1800

[0065] Collect microbial screening samples from pickled shredded radish farmhouses in Fuyuan County, Yunnan. Cut the collected samples into pieces and weigh 1g, put them into 9mL sterile normal saline, shake and mix well, then apply 10-fold gradient dilution on MRS solids culture medium at 37°C for 48h. Observe with the naked eye, pick single colonies of different shapes and sizes in the culture medium, and repeatedly streak and purify them; and then preliminarily identify them as lactic acid bacteria by Gram staining and calcium dissolution method, and store the purified strains in 45% glycerol at -80°C Refrigerator for spare.

[0066] a) Morphological observation

[0067] Streak the purified Lactobacillus fermentum G...

Embodiment 2

[0097] A composition of this embodiment, including Lactobacillus fermentum GF1800 live strain and Lactobacillus fermentum GF1800 postbiotic, wherein, Lactobacillus fermentum GF1800 live strain (22.1 billion CFU / g, batch number: 20210523); Lactobacillus fermentum GF1800 postbiotic : After the fermentation of Lactobacillus fermentum GF1800, it is prepared by inactivation, concentration, and spray drying (the number of cells is 50 billion CFU / g, batch number: 20210524); preferably, the live strain of Lactobacillus fermentum GF1800 and the postbiotics of GF1800 are determined by the number of cells contained in them Calculated, its content ratio is 1:1~5.

[0098] 1) Determination of the ability to degrade nucleosides:

[0099] Lactobacillus fermentum GF1800 was inoculated into MRS liquid medium at an inoculum amount of 5%, activated and cultured at 37°C for 24 hours, and activated twice continuously. Adjust the OD600 of the activated bacterial liquid to 2.0 with physiological sa...

Embodiment 3

[0127] A composition of this embodiment, including Lactobacillus fermentum GF1800 live strain, Lactobacillus fermentum GF1800 live bacteria ≥ 2.0 × 10 10 CFU / g.

[0128] Determination of the nitrite degradation ability of the composition: inoculate the Lactobacillus fermentum GF1800 strain into the MRS liquid medium at an inoculum size of 5%, activate and cultivate at 37°C for 24 hours, and activate twice continuously; Inoculate into MRS liquid medium containing sodium nitrite at an inoculum size of 5%, culture at 37°C in a constant temperature incubator, and take samples every 6 hours to determine the content of sodium nitrite (refer to GB 5009.33-2016 for the determination of sodium nitrite) .

[0129] For the ability of Lactobacillus fermentum GF1800 to degrade nitrite, see Figure 16 . It can be seen that the strain can rapidly degrade nitrite, 30mg / kg nitrite can be degraded to 16.43mg / kg in 6 hours, and the nitrite content can be degraded to less than 1mg / kg in 18 hou...

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Abstract

The invention discloses lactobacillus fermentum for preventing and treating hyperuricemia as well as a composition and application thereof, wherein the lactobacillus fermentum GF1800 for preventing and treating hyperuricemia has the preservation number of CCTCC NO:M 2021984. The lactobacillus fermentum GF1800 is high in biological activity and strong in acid production capacity, can effectively inhibit the activity of pathogenic microorganisms and xanthine oxidase, can tolerate gastric acid and cholate, has a remarkable degradation effect on nucleoside substances, uric acid, cholesterol, nitrite and the like, can efficiently inhibit intake of exogenous uric acid and increase of endogenous uric acid, can obviously reduce the blood uric acid level of hyperuricemia, and meanwhile, improves intestinal and kidney structural injury and inflammatory response. The invention further discloses a composition containing the lactobacillus fermentum GF1800 and a strain metabolite and postbiotics of the lactobacillus fermentum GF1800 and an application of the lactobacillus fermentum GF1800 and the postbiotics of the lactobacillus fermentum GF1800 to preparation of functional food or medicine for preventing and treating hyperuricemia or / and gout, degrading nucleoside, inhibiting xanthine oxidase, reducing cholesterol, degrading nitrite and inhibiting pathogenic bacteria.

Description

technical field [0001] The invention relates to the technical field of microorganisms and food and medicine, in particular to a lactobacillus fermentum for preventing and treating hyperuricemia, its composition and application. Background technique [0002] Hyperuricemia (HUA) is a metabolic syndrome caused by purine metabolic disorders. Hyperuricemia is defined as blood uric acid levels exceeding 420 μmol / L twice on different days in both males and females. Gout is a crystalline arthritis caused by monosodium urate (MSU) deposition, which is directly related to hyperuricemia caused by purine metabolism disorders. In recent years, hyperuricemia in my country has shown a clear upward and younger trend. The overall prevalence of hyperuricemia in China is 13.3%, and the number of patients is about 177 million. The prevalence of gout is 0.86% to 2.20%, of which 0.83% to 1.98% for men and 0.07% to 0.72% for women, and it is increasing year by year (Jiang Quan, et al. Guidelines...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L33/135A61K35/747A61K35/744A61P19/06A61P3/06A61P31/04C12R1/01C12R1/25C12R1/225
CPCC12N1/20A23L33/135A61K35/747A61K35/744A61P19/06A61P3/06A61P31/04A23V2002/00A23V2400/145A23V2400/165A23V2400/173A23V2400/175A23V2400/169A23V2200/30A23V2200/3262A61K2300/00
Inventor 蒲小平刘梅舒梨邓燕闾中平谢建将景晓青卫娟
Owner SICHUAN GAOFUJI BIOLOGICAL TECH
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