Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Microbial agent for promoting root nodule number increase and root nodule nitrogenase activity increase of leguminous crops and application of microbial agent

A technology of microbial bacterial agent and nitrogenase, which is applied in the field of microorganisms, can solve the problems of ineffective bacterial fertilizers, achieve remarkable ecological benefits, be easy to popularize and apply, and improve crop yield and quality

Pending Publication Date: 2022-01-28
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
View PDF8 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the type of strain, nitrogen fixation ability, production technology and effective number of viable bacteria, the effect of bacterial fertilizer is not obvious. At present, it can be applied on a large scale in production and has a significant effect of nodulation and nitrogen fixation, especially inducing legumes to produce "" There is no product with super nodulation phenomenon

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Microbial agent for promoting root nodule number increase and root nodule nitrogenase activity increase of leguminous crops and application of microbial agent
  • Microbial agent for promoting root nodule number increase and root nodule nitrogenase activity increase of leguminous crops and application of microbial agent
  • Microbial agent for promoting root nodule number increase and root nodule nitrogenase activity increase of leguminous crops and application of microbial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1: A kind of preparation method of the microbial bacterial agent that promotes leguminous crops to increase root nodule quantity

[0033] 1. Identification of strains

[0034] The microbial strains involved in the present invention are obtained from peanut pods and rhizospheres in Tangshan, Hebei, and Hezhou, Guangxi through conventional separation and purification of bacteria and molecular identification of 16S rDNA sequences. Among them, the strain of Bacillus amyloliquefaciens BA-HZ54, strain of Bacillus sporogenes BL-TS08 and strain of Bacillus colioids BM-TS05 were preserved in the Chinese Type Culture Collection Center of Wuhan University (CCTCC for short) on October 20, 2021. The deposit numbers are CCTCCNO:M20211295, CCTCCNO:M20211296 and CCTCC NO:M20211297. Enterobacter ludwig was deposited in the China Type Culture Collection Center of Wuhan University on January 7, 2016, with the preservation number CCTCC NO:M 2016014 (Chinese patent application n...

Embodiment 2

[0039] Embodiment 2: the effect of microbial bacterial agent application in promoting leguminous crops such as peanuts to increase root nodule number

[0040] 1. Field test setup

[0041] Field demonstration and application of microbial bacterial agent products was carried out in Zhengyang, Henan, Tangshan, Hebei, Fuxin, Liaoning, Xiangyang, Hubei and other main producing areas of leguminous crops in my country. The test area of ​​each point was 50 mu. Set up a control group and a treatment group, and the treatment group and the control group There is a barrier between groups.

[0042] Control group: an area of ​​25 mu, the variety is the main local planting variety, and the local conventional sowing method is adopted, as well as conventional field management measures such as weeding, pest control, and thriving control.

[0043] Treatment group: The area, variety, sowing method, and field management techniques are the same as those of the control group. On this basis, at the p...

Embodiment 3

[0046] Embodiment 3: The effect of microbial bacterial agent application in improving the nodule nitrogenase activity of peanuts and other leguminous crops

[0047] The field experiment setup is the same as in Example 2.

[0048] Nitrogenase activity reflects the nitrogen-fixing ability of root nodules. In order to further clarify whether peanut root nodules treated with microbial agents have nitrogenase activity, the acetylene reduction method recognized by professionals in the field was used to detect the nitrogenase activity of root nodules in the control group and the treatment group. ,Specific steps are as follows.

[0049] 1) Parameter setting of gas chromatography: the present invention uses the hydrogen ion flame method (FID) to detect the peaks of ethylene and acetylene. See the instrument manual for details on the operation steps. The working parameters of the domestic GC-4000 series gas chromatograph are: vaporization chamber temperature: 120°C; chromatography cha...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of microorganisms, and particularly relates to a microbial agent for promoting root nodule quantity increase and root nodule nitrogenase activity increase of leguminous crops and application of the microbial agent. The microbial agent is prepared by respectively fermenting and culturing four microbial bacteria, namely bacillus amyloliquefaciens, brevibacillus laterosporus, bacillus mucilaginosus and enterobacter ludwigii, concentrating, mixing and compounding. The microbial agent can effectively promote the number of root nodules with nitrogenase activity and increase of nitrogenase activity, promote crop growth and improve crop yield and product quality.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a microbial agent for promoting the number of nodules and the activity of nitrogenase in nodules of leguminous crops and its application. Background technique [0002] Roots of leguminous crops such as peanuts can nodulate and fix nitrogen symbiotically with rhizobia in the soil. When the peanut root system germinates and grows, rhizobia invade through root cracks, stimulate the development of root cortex cells, and gradually form macroscopic nodules on the main root and lateral roots. Rhizobia colonize and reproduce in large numbers in root nodules, and form many spherical bacteroids in a certain period of time, and carry out biological nitrogen fixation through the action of nitrogenase, convert nitrogen in the air into nitrogen fertilizer, and supply nitrogen nutrition to leguminous crops, which not only improves Improve soil fertility, promote high crop yi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20A01G22/40A01G7/06C05G3/00C05G3/80C05G5/12C12R1/07C12R1/01
CPCC12N1/20A01G22/40A01G7/06C05D9/00C05G3/00C05G3/80C05G5/12C05F11/00C05F11/02C05F11/08
Inventor 李培武岳晓凤张奇唐晓倩周扬白艺珍姜俊
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com