Targeted tyrosinase inhibitor, and preparation method and application thereof, and whitening cream

A technology of tyrosinase and inhibitor, which is applied in the field of cosmetics, can solve problems such as application limitations, and achieve the effects of hindering the production of melanin, excellent affinity, and improving chemical stability

Pending Publication Date: 2022-01-21
广州远想医学生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In summary, its application in cosmetics is limited, therefore, it is urgent to develop a protective targeted new tyrosinase inhibitor to improve the whitening effect

Method used

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  • Targeted tyrosinase inhibitor, and preparation method and application thereof, and whitening cream
  • Targeted tyrosinase inhibitor, and preparation method and application thereof, and whitening cream
  • Targeted tyrosinase inhibitor, and preparation method and application thereof, and whitening cream

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Experimental program
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preparation example Construction

[0049]In one of the embodiments, a targeted tyrosinase inhibitor comprises by mass fraction: lecithin 2wt%-10wt%, cholesterol: 1wt%-5wt%, serum albumin: 10wt%-30wt%, tyrosinase Acidase inhibitors: 15wt%-35wt%, mesenchymal stem cell exosomes: 30wt%-60wt%, and the preparation method specifically includes the following steps: mixing mesenchymal stem cell exosomes with lecithin, 5wt% cholesterol, Serum albumin and arbutin are evenly mixed, shaken in ice bath and water bath, frozen at ultra-low temperature, then ultrasonically thawed at room temperature, and repeated freezing and thawing 3-4 times to obtain a targeted tyrosinase inhibitor.

[0050] In one embodiment, the temperature of the ice bath is controlled within the range of -5°C-5°C, the time of the ice bath is controlled within 10 min-30 min, and the time of ultra-low temperature freezing is controlled within 10 min-30 min.

[0051] It can be understood that, in other embodiments, exosomes from mesenchymal stem cells can b...

preparation example 1

[0072] A targeted tyrosinase inhibitor, applicable to the field of cosmetics, comprising the following preparation steps:

[0073] S01. Expand the mesenchymal stem cells in the culture dish to a diameter of 15mm, culture until the cell density reaches about 80%, discard the original medium, and replace it with DMEM containing 0.5wt% exosome-free fetal bovine serum. Base or DMEM / F12 medium, in this embodiment, preferably DMEM / F12 medium, after cultivating for 48h, collect the cell supernatant;

[0074] S02, the cell supernatant was centrifuged under different centrifugation conditions successively, the centrifugation conditions were centrifugation at 1000rpm / min for 10min, 20min at 4000rpm / min, 40min at 10000rpm / min, and the centrifugation temperature was 4°C. Each centrifugation discards the precipitate and collects the supernatant to finally obtain the extract;

[0075] S03. Centrifuge the extract in an ultracentrifuge at a controlled speed of 100,000 rpm / min and a temperatu...

preparation example 2

[0078] A targeted tyrosinase inhibitor, applicable to the field of cosmetics, comprising the following preparation steps:

[0079] S01. Expand the mesenchymal stem cells in the culture dish to a diameter of 15mm, culture until the cell density reaches about 70%, discard the original medium, and replace it with DMEM containing 0.5wt% exosome-free fetal bovine serum for culture Base or DMEM / F12 medium, in this embodiment, preferably DMEM medium, after cultivating for 48h, collect cell supernatant A;

[0080] S02, the cell supernatant was centrifuged under different centrifugation conditions successively, the centrifugation conditions were centrifugation at 1000rpm / min for 10min, 20min at 4000rpm / min, 40min at 10000rpm / min, and the centrifugation temperature was 4°C. Each centrifugation discards the precipitate and collects the supernatant to finally obtain the extract;

[0081] S03. Centrifuge the extract in an ultracentrifuge at a controlled speed of 100,000 rpm / min and a temp...

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Abstract

The invention discloses a targeted tyrosinase inhibitor, and a preparation method and application thereof and whitening cream, and relates to the field of cosmetics. The inhibitor comprises mesenchymal stem cell exosomes, lecithin, cholesterol, serum albumin and a tyrosinase inhibitor, and the preparation method comprises the following steps: mixing the mesenchymal stem cell exosomes with the lecithin, the cholesterol, the serum albumin and the tyrosinase inhibitor, sequentially carrying out ice bath, water bath oscillation and ultralow-temperature cryopreservation, melting at room temperature, and repeatedly freezing and thawing, and obtaining the targeted tyrosinase inhibitor, wherein the targeted tyrosinase inhibitor can be applied to the field of cosmetics. The arbutin is combined with lecithin, cholesterol and serum albumin at the same time, the chemical stability of the arbutin is improved, the mesenchymal stem cell exosome is adopted as a carrier, the permeability in the skin is improved, and therefore the activity of tyrosinase is effectively inhibited, generation of melanin is hindered, and the whitening and freckle removing effects are achieved.

Description

technical field [0001] The invention relates to the field of cosmetics, in particular to a targeted tyrosinase inhibitor, a preparation method and application, and a whitening cream. Background technique [0002] In recent years, cosmetics and skin care products with natural ingredients as the main formula have gradually attracted attention and love. Continuously developing new skin care products with natural ingredients to achieve better skin beautification and skin care effects has become an important research topic in this field. Most cosmetics or whitening agents on the market use tyrosinase inhibitors. Tyrosinase is a key enzyme for producing melanin through melanocytes. Therefore, directly inhibiting the catalytic activity of tyrosinase is the most prominent and effective target. point. Since tyrosinase is only produced by melanocytes, inhibitors targeting tyrosinase can specifically inhibit intracellular melanogenesis without side effects. [0003] For example, arbu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/99A61K8/55A61K8/64A61K8/63A61K8/60A61Q19/02C12N5/0775
CPCA61K8/99A61K8/553A61K8/64A61K8/63A61K8/602A61Q19/02C12N5/0662A61K2800/782A61K2800/5922C12N2509/00
Inventor 欧春凤邹衡芳颜福霞
Owner 广州远想医学生物技术有限公司
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