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Harvesting method for improving quality and yield of Dendrobium officinale Kimura et Migo

A harvesting method and technology of Dendrobium candidum, applied in the directions of botanical equipment and methods, harvesters, agricultural machinery and implements, etc., can solve the problem of inconvenient fertilization and water management, poor erection of Dendrobium candidum, lack of overall growth space, etc. To achieve the effect of improving pharmacological and pharmacodynamic activity, enhancing ventilation and light transmittance, and improving antioxidant and anti-inflammatory activities

Active Publication Date: 2022-01-21
ZHEJIANG SHOUXIANGU PHARMA CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After 3 years of planting with this method, there are also problems similar to the second harvesting method. The overall growth space is insufficient, fertilization and water management are inconvenient, and the uprightness of Dendrobium candidum is not good. Disease, the overall growth of Dendrobium is not good, the quality of Dendrobium is not high, and the shortcomings and deficiencies of low yield
In addition, two to three nodes are left at the bottom of the stem section when harvesting, wasting part of the yield

Method used

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  • Harvesting method for improving quality and yield of Dendrobium officinale Kimura et Migo
  • Harvesting method for improving quality and yield of Dendrobium officinale Kimura et Migo
  • Harvesting method for improving quality and yield of Dendrobium officinale Kimura et Migo

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1: improve the gathering method of dendrobium officinale quality and output

[0042] 1.1 Experimental grouping:

[0043] The present embodiment is divided into experimental group, control group CK1, control group CK2 three groups altogether, and the harvesting method of each group is as follows:

[0044] (1) Experimental group: adopt the method of the present invention to harvest Dendrobium officinale, specifically comprising the following steps:

[0045] 1) Plant new seedlings from March to June, and the new seedlings are divided into tissue culture seedlings and domesticated seedlings;

[0046] 2) In the second year of planting new seedlings, all the old seedlings of tissue cultured seedlings or domesticated seedlings will be harvested from November to December, leaving only the new seedlings; the indicators of old seedlings harvested during this period conform to the 2020 edition of the Chinese Pharmacopoeia;

[0047] 3) In the third year after the new s...

Embodiment 2

[0086]Example 2 Detection of Antioxidant Activity of the Dendrobium officinale Samples Gained in Example 1

[0087] 2.1 Experimental materials

[0088] Dendrobium officinale (prepared by the harvesting method described in Example 1).

[0089] 2.2 Experimental method

[0090] 2.2.1 Scavenging effect on DPPH free radicals

[0091] Take 2 mL of 0.1 mmol / L DPPH absolute ethanol solution, mix it with 2 mL of the sample to be tested, and react in the dark at room temperature for 30 minutes, and the absorbance at 517 nm is measured as Ai; take 2 mL of the sample to be tested and an equal volume of absolute ethanol Mix and react for 30 min, and measure the absorbance at 517 nm as Aj; then take 2 mL of distilled water and mix it with an equal volume of DPPH solution, perform the same operation as above, measure the absorbance at 517 nm as A0, that is, the removal rate of DPPH by the sample to be tested effect.

[0092] Wherein the calculation formula of the scavenging rate of the s...

Embodiment 3

[0098] Embodiment 3 is aimed at the anti-inflammatory activity detection of the dendrobium candidum sample obtained in embodiment 1

[0099] 3.1 Experimental materials

[0100] Dendrobium officinale (prepared by the harvesting method described in Example 1); RAW264.7 cells (SCSP-5036, purchased from the Cell Bank of the Type Culture Collection Committee of the Chinese Academy of Sciences);

[0101] 3.2 Experimental method

[0102] 3.2.1 LPS-induced RAW264.7 cell inflammation model construction

[0103] Take RAW264.7 cells in good growth state and confluence of 90%, inoculate in cell culture plate at 37 ℃, 5% CO 2 The cells were cultured under conditions, and the cells were divided into blank control group, model group and three drug intervention groups. The specific grouping conditions are shown in Table 5.

[0104] Table 5 Grouping of RAW264.7 cells

[0105]

[0106] 3.2.2 RT-PCR detection of IL-6 and IL-1β mRNA expression

[0107] Refer to the instruction manual of E...

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Abstract

The invention relates to a harvesting method for improving quality and yield of Dendrobium officinale Kimura et Migo, which is characterized in that two-stage harvesting is carried out from the third year of new seedling planting, and the growth period of plants is prolonged to 10-13 years; and the two-stage harvesting refers to harvesting of newly germinated plants in the current year from November to December every year, harvesting all 3-4 old seedlings left in each hole in the previous year from May to July every year, and only remaining the newly germinated plants in the current year. Compared with an existing harvesting method of Dendrobium officinale Kimura et Migo, the harvesting method has the advantages that the yield and the quality of the Dendrobium officinale Kimura et Migo are greatly improved, for example, compared with a method for harvesting the Dendrobium officinale Kimura et Migo by retaining part of the mother strips, the yield of polysaccharide of the Dendrobium officinale Kimura et Migo is improved by 30.06%, and the total yield of the Dendrobium officinale Kimura et Migo medicinal material is increased by 62.5%; and compared with a method of harvesting whole plants after planting for 4 years, the method has the advantages that the yield of Dendrobium officinale Kimura et Migo polysaccharides is increased by 73.04%, the total yield of Dendrobium officinale Kimura et Migo medicinal materials is increased by 116.2%, the growth period of plants is prolonged to 10-13 years, and the quality and yield of Dendrobium officinale Kimura et Migo are improved.

Description

technical field [0001] The invention belongs to the technical field of dendrobium candidum harvesting, and in particular relates to a harvesting method for improving the quality and output of dendrobium candidum. Background technique [0002] Dendrobium officinale is a commonly used precious traditional Chinese medicinal material with a long history of application. Traditional Chinese medicine believes that Dendrobium officinale has the functions of nourishing yin and promoting body fluid, invigorating the spleen and stomach, protecting the liver and gallbladder, clearing the throat and improving eyesight, lowering blood sugar, inhibiting tumors, enhancing immunity, relieving fatigue, The efficacy of nourishing the skin and prolonging life has been highly praised by doctors of all dynasties, and it has been recorded and listed as top grade in the medical classics of all dynasties. Modern medical research shows that Dendrobium officinale contains a variety of trace elements, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01D45/00A01G22/00
CPCA01D45/00A01G22/00Y02P60/21
Inventor 李振皓李明焱王瑛蒋燕燕陈泽林赵雁
Owner ZHEJIANG SHOUXIANGU PHARMA CO LTD
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