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Fish skin collagen and extraction method thereof

A fish skin collagen and extraction method technology, which is applied to the preparation method of peptides, chemical instruments and methods, animal/human protein, etc., can solve the problems of high collagen impurities, impurity in fish skin, collagen purity and Low extraction rate and other problems, achieve the effect of maintaining biological activity, filling the gap in the market, and avoiding thermal denaturation

Active Publication Date: 2022-01-18
OCEAN UNIV OF CHINA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The traditional methods for extracting fish skin collagen include hot water extraction, acid extraction, enzyme extraction and alkali extraction, etc. Although the above extraction methods can achieve collagen extraction, most of the conventional extraction methods have impurities in fish skin that cannot be extracted. Clean treatment leads to the problem of more impurities in the extracted collagen, which in turn leads to lower purity and extraction rate of the extracted collagen

Method used

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  • Fish skin collagen and extraction method thereof
  • Fish skin collagen and extraction method thereof
  • Fish skin collagen and extraction method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Take 50g of fresh cod skin with scales and connective tissue removed, and cut it into small pieces of 2cm×2cm with scissors. At 4°C, soak in 2000mL of 0.1M NaOH soaking solution for 24h, change the solution twice during the period, and add 80mL of enzyme activity to the NaOH solution at the last change of solution to obtain 2×10 5 U / g of alkaline protease, washed with distilled water to neutrality after treatment for 4h.

[0054] After adding 100mL of ice-water mixture (including 70mL of ice and 30mL of water) to the fish skin, add it to the wall breaking machine for grinding, run for 10s, pause for 10s, repeat 20 times, and re-add once every 5 times 100mL of ice-water mixture to obtain ground fish skin.

[0055] Add acetic acid and ice water to the ground fish skin until the final concentration of acetic acid is 0.5mol / L, add 0.2g pepsin, stir and extract at 4°C for 24h, then centrifuge at 9500r / min for 30min to get the supernatant.

[0056] Add finely ground NaCl to...

Embodiment 2

[0060] Except that the ground fish skin was extracted in a mixture of acetic acid and pepsin for 36 hours, other steps were the same as in Example 1.

Embodiment 3

[0062] Except that the ground fish skin was extracted in a mixture of acetic acid and pepsin for 48 hours, other steps were the same as in Example 1.

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Abstract

The invention belongs to the technical field of collagen extraction, and particularly relates to fish skin collagen and an extraction method thereof. The extraction method comprises the following steps: performing enzymolysis on fish skin soaked in alkali liquor by using protease, and cleaning the fish skin to be neutral to obtain impurity-removed protein neutral fish skin; crushing the impurity-removed protein neutral fish skin, extracting by acid-enzyme combination method, salting out and dialysis to obtain fish skin collagen. According to the method, the fish skin treated by the alkali liquor is subjected to protease treatment, so that impure protein in the fish skin can be effectively removed, and purity of collagen of the fish skin is effectively improved. Meanwhile, the fish skin is mechanically treated before the collagen of the fish skin is extracted by adopting an acid-enzyme combination method, so that a contact area of the fish skin and acid can be increased, extraction time is shortened, and extraction rate of the collagen is increased.

Description

technical field [0001] The invention belongs to the technical field of collagen extraction, and in particular relates to a fish skin collagen and an extraction method thereof. Background technique [0002] Collagen, also known as collagen, is the most abundant and widely distributed glycoprotein in fish skin. It has attracted widespread attention due to its special right-handed triple helix structure. In food, it is often used as nutritional supplements and adhesives. In terms of tissue engineering, because collagen has good biocompatibility, low antigenicity and degradability, and can promote cell proliferation and other advantages, it perfectly matches the concept of tissue engineering application and is applied to skin wound healing, bone and cartilage tissue Engineering, vascular and nerve regeneration and other fields. [0003] With the progress of society and the improvement of people's living standards, people's demand for aquatic food has increased sharply, which h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K14/78C07K1/30
CPCC12P21/06C07K14/78Y02P20/10
Inventor 王长伟侯虎夏雨刘楚怡
Owner OCEAN UNIV OF CHINA
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