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In-situ efficient compound rhizobium flora

A compound rhizobia and high-efficiency technology, applied in the field of microorganisms, can solve the problems that the addition ratio affects the stability of the flora, affects the function of the flora, and the overlapping of ecological niches, so as to achieve good ecological benefits, improve nitrogen fixation efficiency, and reduce the use of chemical fertilizers and pesticides Effect

Pending Publication Date: 2022-01-18
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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Problems solved by technology

[0005] At present, the research and development of root nodule compound bacteria mainly stays in the artificial combination of different strains for inoculation. There are problems such as competition between bacteria, ecological niche overlap, and functional redundancy in the inoculation of such a combination of bacteria, and different addition ratios will affect the bacteria. The stability of the colony will seriously affect the function of the flora

Method used

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  • In-situ efficient compound rhizobium flora
  • In-situ efficient compound rhizobium flora

Examples

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Embodiment 1

[0017] Embodiment 1: the screening of high-efficiency compound rhizobia group in situ:

[0018] (1) Select well-growing fresh soybean root nodules without disinfection or crushing, put them directly into 200 ml YMA liquid medium, and cultivate them at 28±2°C for 5 days; the compound rhizobia agent YMA liquid medium can be obtained from Purchased from the market or configured according to the following formula, the formula is: mannitol (MYM biological technology company limited) 10 g, yeast powder (OXOID Ltd, LP0021) 3 g, MgSO 4 (Beijing Chemical Plant) 0.2 g, NaCl (Dingguo Biotechnology Development Center) 0.1 g, K 2 HPO 4 (Xilong Chemical Co., Ltd.) 0. 25 g, KH 2 PO 4 (Xilong Chemical Co., Ltd.) 0.25 g, pH 6.8±0.2, distilled water to 1000 mL;

[0019] (2) Take 1ml of the bacteria cultured in (1) and add it to 200ml of YMA liquid medium for subculture, one generation in 5 days, and 50 days in subculture until the pH is stable, and a stable in situ high-efficiency composite...

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Abstract

The invention discloses an in-situ efficient compound rhizobium flora, and belongs to the technical field of microorganisms. The in-situ efficient compound rhizobium flora is prepared by the following method: selecting fresh soybean root nodules which grow well, directly putting the fresh soybean root nodules into 200ml of YMA liquid culture medium without disinfection or crushing, culturing for 5 days at 28 + / -2 DEG C, then sucking 1ml of bacterial liquid, adding the bacterial liquid into 200ml of YMA liquid culture medium for subculture, generating a generation every 5 days, and performing passage for 50 days until the pH is stable to obtain the stable in-situ efficient compound rhizobium flora. The in-situ efficient compound rhizobium flora has the advantages of improving the soybean nodulation efficiency, improving the soybean nitrogen fixation efficiency, reducing the application of chemical fertilizers and pesticides, and having good ecological benefits and wide application prospects.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to an in-situ high-efficiency composite rhizobia group. Background technique [0002] The root nodules on the soybean root system are like "small nitrogen fertilizer plants", which provide soybeans with a steady stream of nitrogen nutrients through biological nitrogen fixation. In production practice, the biological nitrogen fixation function of soybean root nodules can be maximized to provide more nitrogen nutrients for soybean growth and development, so that less or no nitrogen fertilizer is applied, which will save costs and increase efficiency in soybean production and realize the goal of agricultural green development. important meaning. [0003] Over the years, due to the excessive application of nitrogen fertilizers in my country's soybean production, not only did the biological nitrogen fixation function of soybean nodules not play a role, but also increa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/20C12R1/01
CPCC12N1/20
Inventor 马鸣超赵宇宾李俊姜昕关大伟曹凤明李力
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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