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Exosome-loaded GelMA hydrogel microneedle as well as preparation method and application thereof

An exosome and hydrogel technology, applied in the fields of biomedicine and molecular biology, can solve the problems of poor treatment effect, low efficiency of exosome administration, and inability to achieve long-term stable release, so as to reduce the local microenvironment. , the effect of alleviating SCI complications and improving prognosis

Pending Publication Date: 2022-01-18
SHANDONG FIRST MEDICAL UNIV & SHANDONG ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing drug delivery methods tend to cause low efficiency of exosome drug delivery and cannot achieve long-term stable release, resulting in poor therapeutic effect

Method used

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  • Exosome-loaded GelMA hydrogel microneedle as well as preparation method and application thereof
  • Exosome-loaded GelMA hydrogel microneedle as well as preparation method and application thereof
  • Exosome-loaded GelMA hydrogel microneedle as well as preparation method and application thereof

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Experimental program
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Effect test

preparation example Construction

[0072] In yet another specific embodiment of the present invention, a method for preparing the above-mentioned microneedles is provided, the preparation method comprising:

[0073] S1. Culture and preparation of exosomes;

[0074] S2. Add the exosomes prepared in step S1 into the GelMA hydrogel precursor solution, and obtain it after photocuring.

[0075] Wherein, in the step S1, the specific method for culturing and preparing exosomes includes:

[0076] The mesenchymal stem cells were cultured in 2D or 3D, and the exosomes secreted by the mesenchymal stem cells were collected by centrifugation.

[0077] In yet another specific embodiment of the present invention, the 3D culture specifically includes: adding mesenchymal stem cells into the GelMA precursor solution, adding the cell solution into the curing ring, adding complete medium after photocuring, and short-term (such as 1 -10min, preferably 5min) after culturing, remove the culture medium, then add a new culture medium...

Embodiment

[0092] 1. Experimental animals: Adult male SPF grade SD rats weighing 280-320 g were selected for this study, and the experimental animals were purchased from Jinan Pengyue Experimental Animal Breeding Co., Ltd. They were kept in the SPF-grade animal breeding room of the Animal Center of the First Affiliated Hospital of Shandong First Medical University, with a temperature of 20±2°C and a natural light / dark (≈12h-12h) cycle. Animal experiment protocols were approved by the Animal Care and Use Committee of Shandong First Medical University according to the principles outlined in the Animal Care and Use Guide. Personnel working with animal models received systematic training in accordance with Institutional Animal Care and Use Committee guidelines (IACUC).

[0093]2. Rat SCI model establishment: The spinal cord injury model was established by the clamp method. The specific method was: all rats were fasted for 6 hours before operation, anesthetized with isoflurane, and the dose o...

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Abstract

The invention provides an exosome-loaded GelMA hydrogel microneedle as well as a preparation method and application thereof, and belongs to the technical field of biological medicines and molecular biology. The invention researches and verifies that the exosome microneedle (GelMA-MNs-MSC-Exo) derived from MSC loaded by GelMA hydrogel has the effects of relieving SCI complications, improving prognosis and the like for the first time. Specifically, the GelMA-MNs-MSC-Exo can effectively improve the symptoms such as neurological impairment after SCI, so that the GelMA-MNs-MSC-Exo is a potential treatment method, can relieve the local microenvironment after SCI and promote the recovery of neurological functions, and has good practical application value.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and molecular biology, and in particular relates to a GelMA hydrogel microneedle loaded with exosomes and a preparation method and application thereof. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Spinal cord injury (SCI) is a central nervous system injury disease with high disability rate, leading to spinal cord nerve cell death and glial scar formation, motor, sensory and autonomic dysfunction and neuralgia. Severe consequences of SCI can bring enormous physical and psychological stress and huge medical burden to patients. In addition to supportive treatment and emergency surgery, the...

Claims

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Application Information

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IPC IPC(8): A61K35/28A61K9/00A61K47/42A61P25/00C12N5/0775A61M37/00
CPCA61K35/28A61K9/0021A61K47/42A61P25/00C12N5/0663A61M37/0015A61M2037/0023A61M2037/0046A61M2037/0053A61M2037/0061
Inventor 辛涛韩敏王晓峰
Owner SHANDONG FIRST MEDICAL UNIV & SHANDONG ACADEMY OF MEDICAL SCI
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