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A defensin-like immunomodulatory tetradeceptide rv14 and its preparation method and application

An immune regulation, RV14 technology, applied in the biological field, can solve the problem of no antibacterial activity, achieve good antibacterial activity, do not cause hemolysis, and reduce costs

Active Publication Date: 2022-07-15
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has good immunomodulatory activity, but no bacteriostatic activity in vitro or in salt ion solution

Method used

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  • A defensin-like immunomodulatory tetradeceptide rv14 and its preparation method and application
  • A defensin-like immunomodulatory tetradeceptide rv14 and its preparation method and application
  • A defensin-like immunomodulatory tetradeceptide rv14 and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Example 1 Design and synthesis of antimicrobial peptides

[0014] Step 1: Through the alignment of defensin sequences, a central skeleton CxxGyC with defensin characteristics is obtained, where x is a cationic amino acid, y is a hydrophobic amino acid, and the immunomodulatory characteristics of beta defensin are retained as much as possible, and the skeleton sequence is short. , which is more conducive to practical synthesis and application. Through the screening of therapeutic index, RV14 with the highest therapeutic index was selected from the 14 peptides as the target peptide. Its skeleton is CRRGVC.

[0015] Step 2: Add WRWR and RWRW amino acid sequences to the N- and C-termini of the backbone. This fragment has good antibacterial activity, which makes it have good antibacterial activity, and at the same time, it is amidated at the C-terminus to increase its cationicity and stability. The final sequence is WRWRCRRGVCRWRW-NH 2 .

[0016] Step 3: Antibacterial p...

Embodiment 2

[0020] Example 2 Secondary structure analysis of antimicrobial peptides

[0021] The secondary structures of antimicrobial peptides were analyzed by CD spectroscopy. The results showed that in the water environment, the peptide RV14 assumed a β-sheet structure and still retained the β-defensin fold structure. It is proved that the designed peptide has a strong structure, which is different from other peptides showing random coil structure in water, RV14 can show structure even in water. Means it can also function in water. In contrast, in the hydrophobic environment (50% TFE) mimicking microbial membranes and in the negatively charged prokaryotic membrane environment (30 mM SDS), the β-sheet structure of the peptides was weaker. Most antimicrobial peptides exhibit random coils in the aqueous environment, but exhibit structures in the cell membrane environment.

Embodiment 3

[0022] Example 3 Salt ion stability of antimicrobial peptides

[0023] Salt ions are added to the BSA solution to configure BSA solutions with different salt ions. And the antibacterial activity was measured again according to the method of antibacterial activity. The final concentrations of salt ions measured were: 150 mM NaCl, 4.5 mM KCl, 6 mM NH 4 Cl, 8mM ZnCl 2 , 1mM MgCl 2 , 2.5mM CaCl 2 and 4mM FeCl 3 . The results are shown in Table 2, the antimicrobial peptides have good stability in the seven salt ions. Except in the sodium ion environment, the antibacterial effect was slightly weakened, and the antibacterial activity remained unchanged in the other six salt ion environments.

[0024] Table 2 Antibacterial activity of RV14 against bacteria in salt ion environment

[0025]

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PUM

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Abstract

The present invention relates to a defensin-like immunoregulatory tetradeceptide RV14, a preparation method and application thereof, and the sequence of the antibacterial peptide RV14 is shown in SEQ ID No.1. By comparing and analyzing the amino acid sequences of the β-defensin family, the common amino acid sequences of the β-defensin family were screened out. Through the transformation, a β-defensin fragment CRRGVC containing a pair of disulfide bonds was designed, and then the N-terminus of the β-defensin fragment CRRGVC was designed. The WRWR amino acid sequence and the C-terminal RWRW amino acid sequence were used for sequence addition, and the carboxyl terminal of the peptide was amidated to synthesize the antimicrobial peptide RV14 by chemical methods. Antibacterial peptide RV14 has a greater improvement in antibacterial activity, does not cause hemolysis, and has immunomodulatory activity. It shows that the addition of functional fragments can enhance the antibacterial activity of antibacterial peptides, improve cellular immune regulation, further verify its antibacterial mechanism, and provide a theoretical basis for the application of antibacterial peptides.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a defensin-like immunoregulatory tetradeceptide RV14 and a preparation method and application thereof. Background technique [0002] Although antibiotics have always been effective drugs for human treatment of various diseases caused by pathogenic bacterial infections. However, with the large-scale abuse of antibiotics, the animal body becomes resistant to resistance, and the era of antibiotics is gradually coming to an end. Therefore, finding new alternatives to antibiotics has become the focus of attention. Antimicrobial peptides (AMPs) exist in a variety of organisms in nature. It has broad-spectrum antibacterial activity, and because of its special antibacterial mechanism, it is not easy to develop drug resistance, and has great potential to replace the use of antibiotics. However, there are still some shortcomings that limit the application. For example, the more...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/47A61K38/17A61P31/04
CPCC07K14/4723A61P31/04A61K38/00
Inventor 窦秀静赵聪李洋单安山李仲玉
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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