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Modular tissue-engineered bone-ligament-bone graft and preparation method thereof

A tissue-engineered bone and bone graft technology, applied in the medical field, can solve the problems of in-depth understanding, non-union, and lack of high-efficiency and high-quality ligament injury

Pending Publication Date: 2022-01-07
SHANGHAI SIXTH PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current treatment methods often lead to scar healing of ligament tissue, or even non-union. The main reason is that the current mechanism of ligament injury healing and regeneration is not well understood, and there is currently a lack of efficient and high-quality tendon grafts or tendon repairs for repairing ligament injuries. thing

Method used

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  • Modular tissue-engineered bone-ligament-bone graft and preparation method thereof
  • Modular tissue-engineered bone-ligament-bone graft and preparation method thereof
  • Modular tissue-engineered bone-ligament-bone graft and preparation method thereof

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Embodiment

[0032] Such as Figure 1-2 As shown, the present embodiment provides a modular tissue engineered bone-ligament-bone graft, the preparation steps of which include:

[0033] S1. Take 50 mL of peripheral blood, anticoagulate and centrifuge in vitro for 15-20 minutes (without adding thrombin) to obtain platelet-rich plasma; mix the platelet-rich plasma with fibrin at a ratio of 1:1 to obtain platelet-rich plasma - fibrin gel; using electrospinning technology, the collagen is prepared into an oriented collagen fiber membrane, and the thickness of the oriented collagen fiber membrane is 0.02-0.2mm; gradient chemical precipitation is used at both ends of the oriented collagen fiber membrane, mixed with The impurity thickness is 0.02-0.2mm, the density is 0.01-1g / cm 3 β-tricalcium phosphate (doped into the density of β-tricalcium phosphate gradually decreases from the end to the middle) to make a collagen-tricalcium phosphate film; on the collagen-tricalcium phosphate film, platelet-...

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Abstract

The invention relates to a preparation method of a modular tissue-engineered bone-ligament-bone graft, which comprises the following steps: doping beta-tricalcium phosphate at two ends of an oriented collagen fiber membrane by adopting a gradient chemical precipitation method, and gradually reducing the density of the doped beta-tricalcium phosphate from the end part to the middle to prepare a collagen-tricalcium phosphate membrane; coating the collagen-tricalcium phosphate membrane with platelet-rich plasma-fibrin gel to prepare a bone-ligament-periosteum; inoculating seed cells on the bone-ligament-periosteum, culturing in vitro to prepare a tissue-engineered bone-ligament-periosteum; and overlapping and compounding a plurality of tissue-engineered bone-ligament-periosteum to obtain the tissue-engineered bone-ligament-bone graft. The invention constructs a simulated bone-ligament-bone organoid structure, explores the molecular mechanism of ligament injury and tendon-bone interface healing in vitro, and further utilizes the organoid to prepare a tissue-engineered bone-ligament-bone graft for ligament injury and tendon-bone interface tissue repair and regeneration.

Description

technical field [0001] The invention relates to the medical field, in particular to a modular tissue engineering bone-ligament-bone graft and a preparation method thereof. Background technique [0002] Soft tissue injuries of ligaments (such as cruciate ligaments, collateral ligaments, etc.) are very common sports injuries, and 50% of injuries in youth sports are ligament sprains. Meanwhile, the incidence of anterior cruciate ligament (ACL) injuries has increased by 1.3 percent over the past decade. Except for sports people, the probability of tendon and ligament injury in diabetic patients is ten times that of non-diabetic patients. As we age, 80% of older adults experience ligament ruptures and tendon injuries. Although tendon and ligament injuries are very common and have a huge impact on individuals, families, and the economy, limited progress has been made in prevention and repair after injury. [0003] At present, the prevention and treatment of ligament injuries ma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/46A61L27/28A61L27/34A61L27/36A61L27/38A61L27/50
CPCA61L27/46A61L27/34A61L27/28A61L27/3616A61L27/3843A61L27/50A61L2430/10A61L2430/02A61L2430/06C08L89/00
Inventor 赵金忠李亚民崔文国蒋佳赵松
Owner SHANGHAI SIXTH PEOPLES HOSPITAL
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