Application of culture medium in oudemansiella raphanipies
The technology of an original culture medium and culture medium, which is applied to the application in the field of the cultivation medium, can solve the problems such as the limitation of development and utilization, the toxicity of antioxidants, and the insecurity of the cockroach. Theoretical significance and application value, the effect of promoting accumulation and improving protein content
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[0029] A kind of cultivation method of P. nigella comprises the following steps:
[0030] 1) Preparation of culture medium
[0031]The stock medium includes the following components: wheat bran 35g / L, glucose 20g / L, dipotassium hydrogen phosphate 3.5g / L, magnesium sulfate 2g / L, agar 20g / L, pH 6.5. Weigh the required wheat bran, glucose, dipotassium hydrogen phosphate and magnesium sulfate respectively according to the formula. Wrap the weighed wheat bran with 6-8 layers of gauze and put it into a cooking pot, add 200mL of water to boil, then time it for 15-30 minutes, then filter it with 6-8 layers of gauze, pour the filtrate into a 500mL large beaker, Add other weighed medicines one by one, stir with a glass rod until dissolved, measure enough water and pour it into a beaker to make up the volume. Use 1 mol / L hydrochloric acid solution to adjust the pH of the culture medium to 6.5, divide into test tubes, seal them, put them in a high-pressure steam pot for sterilization, a...
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