Method for inducing pinellia ternate callus and special culture medium of method

A technology for inducing culture medium and callus, applied in the field of plant tissue culture, can solve the problems of rarely using seed propagation methods, less large-scale planting area, limited Pinellia ternata, etc.

Active Publication Date: 2022-01-04
YIBIN UNIV
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Problems solved by technology

But despite this, the "Shui Pinellia" that was seized still became popular instead of Pinellia. In addition, due to the particularity of pinellia reproduction, a large amount of seeds is required for its tuber reproduction, especially in continuous cropping, it is very easy to cause virus infection, and the disease is very serious during heavy cropping, and the quality and yield are greatly reduced, resulting in planting hysteresis
On the other hand, as far as the market itself is concerned, the demand for Chuan Pinellia is also growing continuously. The reality of the shortage of supply has led to the excessive use of "Water Pinellia" and cannot be contained, which greatly limits the development of the Sichuan Pinellia industry.
[0004] Although the artificial cultivation of pinellia can achieve partial success, if you want to expand the planting, there are still pr...

Method used

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  • Method for inducing pinellia ternate callus and special culture medium of method
  • Method for inducing pinellia ternate callus and special culture medium of method
  • Method for inducing pinellia ternate callus and special culture medium of method

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Embodiment 1

[0021] A method for inducing Pinellia pinellia callus, comprising the following steps:

[0022] 1. Disinfection of explants

[0023] Rinse the petioles or leaves with running water for 20 minutes, then soak them in 0.2% carbendazim for 30 minutes, then disinfect them with 75% alcohol for 45 seconds, rinse them with sterile water for 2-3 times, and finally place them in a sterile container. In the inoculation tray of filter paper, dry the petiole or leaf surface moisture, and finally cut the petiole into small sections of about 1.0 cm, and cut the leaves into small squares of about 0.5 cm × 0.5 cm.

[0024] 2. Induction Medium Preparation

[0025] The preparation of pinellia callus induction medium comprises the following steps:

[0026] Using MS medium as the basal medium, add 1.0mg / ml of 6-BA and 0.5mg / ml of NAA, and prepare camphor essential oil media containing different concentrations of camphor essential oil in the following table.

[0027]

[0028]

[0029] 3. I...

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Abstract

The invention discloses a method for inducing pinellia ternate callus and a special culture medium of the method, and belongs to the technical field of plant tissue culture. According to the method, pinellia ternate explants are placed on an induction culture medium to be subjected to induction culture, calluses which grow rapidly and are vigorous are obtained, an MS culture medium serves as a basic culture medium of the induction culture medium, and 6-BA, NAA and cinnamomum longepaniculatum essential oil are added into the induction culture medium. The stable and efficient induction method of the pinellia ternate callus is established, the yield and quality of the callus are improved, the callus is normal in subculture proliferation, and important technical support is provided for further development of pinellia ternate breeding research.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and more specifically relates to a method for inducing pinellia callus and a special culture medium thereof. Background technique [0002] Pinellia belongs to the genus Pinellia of the family Araceae. It is a perennial herb, also known as wild taro, triple jump, etc. It is recorded in the treasure house of traditional Chinese medicine as an important Chinese medicinal material, and its origin is in my country and Japan. Pinellia is used as medicine after the tubers are dried. Pinellia is mainly distributed in Yunnan, Jiangnan, Sichuan, Guizhou, Hubei and other places in China. According to survey statistics, among the 558 traditional Chinese medicine prescriptions, Pinellia is the 22nd most frequently used. [0003] The sandy soil of Pinellia is generally excavated in summer and autumn, and the tubers are spherical or oblate. It is a traditional Chinese medicinal material that is ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/002A01H4/00
Inventor 武彦芳张建马金鹏王宇朱梦鑫赵善梅
Owner YIBIN UNIV
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