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Reagent and kit for detecting Kawasaki disease and evaluating curative effect and application

A technology for detection kits and efficacy evaluation, applied in biological testing, disease diagnosis, measurement devices, etc., can solve problems such as lag and complex Kawasaki disease diagnosis, and achieve the effect of increasing sensitivity, improving medical capacity, and improving accuracy

Pending Publication Date: 2021-12-24
胜亚生物科技(厦门)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the absence of specific diagnostic methods at present, the clinical diagnosis of Kawasaki disease is quite complicated and lags behind

Method used

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  • Reagent and kit for detecting Kawasaki disease and evaluating curative effect and application
  • Reagent and kit for detecting Kawasaki disease and evaluating curative effect and application
  • Reagent and kit for detecting Kawasaki disease and evaluating curative effect and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] CXCL10 and CCL23 ELISA plate preparation

[0054] In this embodiment, the leading reaction solution required for the ELISA reaction was prepared according to the specific components and conditions listed in Table 1 to Table 3 below. The CXCL10 coating solution and the CCL23 coating solution were added to different 96-well plates respectively; the coating solution was discarded, and the sealing solution was added to each well to seal; the sealing time was 2 hours at room temperature, and after 5 hours of air drying, together with The desiccant is stored in an aluminum foil bag and refrigerated.

[0055] Table 1 Preparation of CXCL10 coating solution components

[0056] components final concentration per reaction PBS buffer solution (pH=7.4) 1× CXCL10 monoclonal antibody 1μg / mL

[0057] Note: The commercial information of the CXCL10 monoclonal antibody is: manufacturer: invitrogen, product name: CXCL10 Polyclonal Antibody, product number: PA...

Embodiment 2

[0064] ELISA operation and result analysis

[0065] Then, perform the ELISA operation according to the following steps.

[0066] 1. Reagent preparation: Take out the components of the kit (including the microplate prepared in Example 1), and equilibrate for about 30 minutes at room temperature (18-25°C). After the microplate is opened, take the required amount, and the rest should be timely Seal up and store at 2-8°C.

[0067] In this embodiment, the total number of test samples is 8 cases, and the samples are prepared according to the reagent composition list listed in Table 4. If the number of detected samples is 16 cases, then the components listed in Table 4 will be increased twice. and so on.

[0068] Table 4 Reagent Component Preparation Table

[0069]

[0070] Among them, the 10-fold sample dilution solution contains PBS buffer solution and BSA; the enzyme labeling solution is horseradish peroxidase; the 10-fold washing solution contains PBS buffer solution and T...

Embodiment 3

[0099] Reagent Performance Verification

[0100] In this embodiment, 10 existing positive reference products and 4 negative reference products were obtained, and double verification was carried out on the detection reagent of the present invention and the aforementioned judgment methods. The results show that the detection reagent of the present invention and its judgment criteria all meet the positive and negative requirements of the reference product (Table 7 and Table 8).

[0101] Table 7-1 Concentration and Judgment Results of Positive Reference Substances CXCL10 and CCL23

[0102]

[0103] Table 7-2 Judgment Results of Positive Reference Substances

[0104]

[0105] Table 8-1 Concentration and Judgment Results of Negative Reference Substances CXCL10 and CCL23

[0106]

[0107]

[0108] Table 8-2 Judgment Results of Negative Reference Substances

[0109]

[0110] According to the test results, by comparing the CXCL10 concentration and the CCL23 concentratio...

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Abstract

The invention relates to a reagent and kit for detecting the Kawasaki disease and evaluating a curative effect, and application, and belongs to the technical field of kit preparation. The reagent for detecting the Kawasaki disease and / or evaluating the curative effect comprises a reagent for detecting a biomarker amount of a biomarker, wherein the biomarker comprises CCL23 and / or CXCL10. The reagent disclosed by the invention can be used for detecting the Kawasaki disease and evaluating the curative effect of a therapeutic machine for treating the Kawasaki disease, and can improve the Kawasaki disease detection sensitivity and accuracy.

Description

technical field [0001] The invention relates to the technical field of kit preparation, in particular to a reagent, kit and application for detecting Kawasaki disease and evaluating curative effect. Background technique [0002] Kawasaki disease is a multisystem vascular inflammatory syndrome of unknown etiology. Japanese pediatrician Tomisaku Kawasaki discovered the first case of children with oral mucosal, skin and lymph node lesions in 1961. Since then to 1967, more than 50 similar cases have been discovered one after another; To commemorate Tomisaku Kawasaki The contribution of doctors, so this disease is called Kawasaki disease (Kawasaki disease), also known as mucocutaneous lymph node syndrome (mucocutaneous lymph node syndrome, MCLS) or Kawasaki syndrome (Kawasaki syndrome). 85% of children with Kawasaki disease are younger than five years old; the ratio of male to female patients is about 1.5:1. According to epidemiological statistics, the country with the highest ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68C07K16/18
CPCG01N33/6893C07K16/18G01N2800/32
Inventor 林伯泽郭亦亦邱一帆
Owner 胜亚生物科技(厦门)有限公司
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