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Magnetic graphene nano composite gel for targeted inhibition of corneal lesion, preparation method and application of magnetic graphene nano composite gel, and treatment method

A magnetic graphene and nano-composite technology, applied in treatment, magnetic therapy, electrotherapy, etc., can solve the problems of high toxicity and side effects, high magnetic field strength, poor treatment effect, etc., to inhibit expression, eliminate ROS, and improve targeting effect with effectiveness

Active Publication Date: 2021-12-24
WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current magnetic field stimulation technology that can be used clinically has significant disadvantages such as large toxic and side effects, unclear targets, and poor therapeutic effects due to high magnetic field strength (>1T), making magnetic stimulation technology only available in transcranial magnetic stimulation. and other limited fields have been applied to a certain extent

Method used

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  • Magnetic graphene nano composite gel for targeted inhibition of corneal lesion, preparation method and application of magnetic graphene nano composite gel, and treatment method
  • Magnetic graphene nano composite gel for targeted inhibition of corneal lesion, preparation method and application of magnetic graphene nano composite gel, and treatment method
  • Magnetic graphene nano composite gel for targeted inhibition of corneal lesion, preparation method and application of magnetic graphene nano composite gel, and treatment method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A kind of nano gel material of the magnetic suppression technology that is used for non-invasive suppression corneal inflammation, its preparation method comprises the following steps:

[0028] Mix graphite powder and carboxylated polymer in a mass ratio of 1:5-1:10 and place them in a ball mill jar. Ball milling at room temperature for 3-5 hours at a rotational speed of 350-500 rpm to obtain hydrophilic graphene nanosheets. Subsequently, by co-precipitation method, the magnetic nanoparticles Fe 3 o 4 Precipitate to graphene nanosheets to obtain magnetic graphene nanocomposites. Such as figure 1 As shown in (a), the prepared magnetic nanocomposite is brownish yellow and has good magnetic properties. The nanocomposite can be extracted from the solution by adding a magnet. The synthesized magnetic nanocomposites were combined with sodium hyaluronate viscoelastic ( figure 1 b) Ultrasonic mixing, prepared as figure 1 (b) Nanogel solution shown. figure 1 The transmiss...

Embodiment 2

[0030] Primary mouse corneal endothelial cells (MCEC) were planted on a cell culture plate at a density of 5,000-10,000 cells / well and incubated for 24 hrs, and the magnetic nanogel (5-50 μg / mL) obtained in Example 1 was added to the experimental group Co-culture up to 96 hrs. Cell viability was detected by CCK-8 method. Such as figure 2 As shown, in the highest dose group (50 μg / mL), the co-culture of cells and nanomaterials lasted up to 96 hrs, and the relative survival rate of cells was still greater than 100%, indicating that the prepared nanomaterials had good biocompatibility.

Embodiment 3

[0032] The corneal inflammatory cell model was established by planting the same amount of MCEC cells on different cell culture plates, incubating them for 24 hrs until they adhered to the wall, and then adding a certain amount of lipopolysaccharide (LPS) to induce them for 24 hrs to establish the model. Such as image 3 As shown, the magnetic graphene nanogel prepared in Example 1 was added to the cell model, and after overnight, after the cells had naturally settled around the cells, the culture plate was placed in an instrument surrounded by a magnetic field for the magnetic inhibition test .

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Abstract

The invention relates to a magnetic graphene nano composite gel for targeted inhibition of corneal lesion, a preparation method and application of the magnetic graphene nano composite gel, and a treatment method. The magnetic graphene nano composite gel is characterized in that a high-performance magnetic nano gel material is introduced into cells to serve as a magnetic induction receptor, a low-intensity (less than 30 mT) remote magnetic field inhibition technology is adopted, the expression of corneal endothelial inflammation related factors can be efficiently inhibited, the targeting property and the effectiveness of the magnetic inhibition effect can be substantially improved, the ROS in an inflammatory cell model can be efficiently eliminated, the expression of the inflammatory factors can be inhibited, the development of the corneal endothelial inflammation can be regulated from the molecular level, and a new strategy can be provided for the clinical treatment of corneal inflammation related diseases.

Description

technical field [0001] The invention relates to the technical field of nano-biological materials and tissue engineering, in particular to a magnetic graphene nano-composite gel for targeted inhibition of corneal lesions, its preparation method, application and treatment method. Background technique [0002] The cornea is a transparent, avascular fibrous membrane located on the outermost wall of the eyeball and plays a very important role in maintaining normal vision in the eyeball. However, due to the special position of the cornea, it is easily affected by external pathogenic microorganisms and chemical substances, resulting in keratitis, corneal ulcer, and eventually functional blindness. Corneal disease is the third leading cause of blindness in the world after cataract and glaucoma, and the patients are mainly young and middle-aged, which has brought a huge burden to society. Corneal disease, especially corneal disease caused by corneal endothelial inflammation, is a ma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/06A61K41/00A61K47/02A61K47/04A61K47/36A61N2/00A61P27/02A61P29/00A61P31/04A61P31/10
CPCA61K9/06A61K47/36A61K47/02A61K9/0048A61K9/0019A61P27/02A61P31/10A61P31/04A61P29/00A61K41/0052A61N2/004Y02A50/30
Inventor 晏露刘勇王乐锋黄萍萍张文晶
Owner WENZHOU MEDICAL UNIV
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