Magnetic micro-particle chemiluminescence kit for determining content of interleukin-8 in human serum

Abstract

The invention relates to a magnetic micro-particle chemiluminescence kit for determining the content of interleukin-8 in human serum. The magnetic micro-particle chemiluminescence kit comprises a reagent R1, a reagent R2, a magnetic separation reagent, a calibration product and a quality control product, wherein the reagent R1 is prepared from a biotinylated IL-8 monoclonal antibody A and a reagent R1 diluent, the reagent R2 is prepared from an alkaline phosphatase labeled IL-8 monoclonal antibody B and a reagent R2 diluent, the magnetic separation reagent is prepared from commercial streptavidin magnetic beads and a magnetic separation reagent diluent, the calibration product and the quality control product are prepared from diluents containing IL-8 antigens with different concentrations and proteins, and a chemiluminescent substrate solution required in an experiment process is an alkaline phosphatase catalytic luminescence substrate solution. According to the invention, a chemiluminescence detection technology and a biotin-streptavidin system are combined for the first time to realize the purpose of quantitative detection of the content of IL-8 in a human serum sample, and a more accurate, precise, convenient, rapid and simple method is provided for clinical detection of IL-8 in human serum.

Description

technical field [0001] The invention relates to the field of medical diagnostic reagents, in particular to a magnetic particle chemiluminescence reagent for detecting the content of interleukin-8 (IL-8) in human serum by combining the principle of chemiluminescence detection technology with biotin-streptavidin system Boxes and methods of making them. Background technique [0002] Interleukins are a type of cytokines that are produced by and act on various cells. At least 38 interleukins have been discovered so far, named respectively as IL-1~IL-38, with complex functions; they play an important role in a series of processes such as the maturation, activation, proliferation and immune regulation of immune cells, and they also participate in Various physiological and pathological reactions of the body. The proliferation, differentiation and function of immune system cells are regulated by a series of cytokines. According to the structural homology of cytokines, they can be ...

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Problems solved by technology

[0006] At present, the detection of IL-8 adopts the double-antibody sandwich ELISA method. In this method, the biotinylated anti-human IL-8 antibody binds to IL-8, and horseradish peroxidase is used to label avidin. Biotin and avidin are specific In combination, horseradish peroxidase catalyzes the oxidation of the colorless chromogenic agent into blue. This enzyme-linked immunosorbent assay has a low degree of automation and complicated operation. The magnetic particle chemiluminescence immunoassay reagent of the horseradish peroxidase system The stability needs to be improved

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