Nematode extract and application thereof
An extract and nematode technology, applied in the field of pesticides, can solve the problems of high toxicity, general pine wood nematode inhibitory effect, need for further research, etc., to achieve the effect of easy acquisition and improved activity
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Embodiment 1
[0116] Embodiment 1, the preparation of the crude extract of pine xylophilus and its chemical composition analysis
[0117] 1 The source of the tested pine xylophilus and the expanded culture of the pine xylophilus
[0118] Weigh 300g of dry corn kernels with complete shape and soak them in water for 2 days at room temperature, then pack them into 10 Erlenmeyer flasks with a capacity of 250mL. Bacteria for 30 minutes. After the sterilization is completed, wait for it to cool naturally to room temperature, inoculate an appropriate amount of Botrytis cinerea under aseptic conditions, and culture it in an artificial climate box at 25°C and 70% RH in the dark for 5-7 days, and then pour it into each Erlenmeyer flask Inoculate 3000-5000 pine wood nematodes, and continue culturing in an artificial climate chamber at 25° C. and 70% RH in the dark for 8-15 days to harvest a large number of pine wood nematodes.
[0119] 2 Preparation of Crude Extract of Pine Xylophilus
[0120] Afte...
Embodiment 2
[0134] Embodiment 2, the activity experiment of pine wood nematode crude extract to pine wood nematode
[0135] Drug configuration: the nematode crude extract prepared in Example 1 is configured into a 10g / L aqueous solution with the DMSO test system (4%DMSO+15% Tween 80), and the DMSO test system (4%DMSO+15% Tween 80 ) was the blank control group.
[0136] Preparation of the nematode suspension: suspend the pine wood nematode on the corn grain medium with an appropriate amount of sterilized water, and then separate the living pine wood nematode suspension by the Bellman funnel method, and dilute the nematode suspension at 2000r / Centrifuge for 2 min, remove the supernatant and keep the sediment, add appropriate amount of sterilized water, observe the nematode age, activity status and count the number of nematodes under the microscope, and finally quantify the nematode suspension to 1000 nematodes / mL with sterilized water. The nematodes are in the J3 stage, and a few are in t...
Embodiment 3
[0141] Embodiment 3, the activity test of the active ingredient monomer contained in the pine wood nematode extract to pine wood nematode
[0142] Experiment 1. Activity and LC50 determination of azelaic acid against pine xylophilus
[0143] (1) Activity experiment of azelaic acid (DMSO / Tween water system) on pine xylophilus
[0144] Pharmacy preparation:
[0145] The preparation of the following experimental group medicaments is as follows: Azelaic acid (commercially available) is prepared through an aqueous solution containing DMSO and Tween 80 to prepare different concentrations of azelaic acid aqueous solutions; wherein, the content of DMSO in the aqueous solution is 4%, and the content of Tween 80 15%.
[0146] test group:
[0147] 1) 10 mL of azelaic acid aqueous solution with a concentration of 7 g / L;
[0148] 2) 10 mL of azelaic acid aqueous solution with a concentration of 5 g / L;
[0149] 3) 10 mL of azelaic acid aqueous solution with a concentration of 3 g / L;
...
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