T cell receptors and methods of use thereof
A cell receptor, NY-ESO-1TCR technology, applied in animal cells, genetically modified cells, using vectors to introduce foreign genetic material, etc., can solve problems that hinder the comprehensive analysis of anti-tumor T cell specificity
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[0208] TILs were isolated from metastatic melanoma patients, followed by polyclonal expansion in vitro and examined for NY-ESO-1 antigen specificity for the HLA-B*07:02 allele. A combination of structure-based and functional assays using peptide / HLA (pHLA) multimers has been used to measure antigen-specific T cell responses. Using pHLA multimers with previously known NY-ESO-1 60-72 The peptides stained T cells (Figure 1). TIL shows to B*07:02 / NY-ESO-1 60-72 Multimers were positive. According to ELISPOT analysis, multimer-positive T cells secreted detectable IFN-γ in an HLA-restricted peptide-specific manner ( figure 2 ).
[0209] Multimer-positive anti-tumor T cells were collected and their TCR genes were molecularly cloned (Figure 3). Antigen specificity and functional reactivity of cloned TCRs were verified by multimeric staining and ELISPOT assay of TCR-reconstituted T cells. When reconstituted on primary T cells, B*07:02 / NY-ESO-1 60-72 TCR-transduced T cells were s...
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